FastQCFastQC Report
Thu 26 May 2016
SRR1926708_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926708_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858733
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11210.13054115772888664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG602.191682E-517.8531971
CTGTAGG7100.016.595931
GCCTTAG604.428176E-415.6215481
TAGGACC4050.015.4343374
TGTAGGA7800.015.16430952
GTCTAAG550.004473160514.60716151
GTAGGAC8000.014.1826053
GTCCTAC7200.014.133781
TAGGTCC450.005968040313.9868895
TGTCGTT400.00652937913.79875910
TATAAGG700.00116222913.7536762
CTATACT952.2422864E-713.613594
TAAGGAG851.2930359E-613.5755115
GATCTAC700.001422826413.3898971
GTCTTAC700.001422826413.3898971
GTCCTAT802.553777E-413.3898961
TATACTG1202.2791937E-913.112715
CTACACT1900.013.0463574
TACTAGA853.399004E-412.9446362
GTGTAGA1152.4837227E-612.8077281
Produced by FastQC (version 0.11.5)