FastQCFastQC Report
Thu 26 May 2016
SRR1926708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926708_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858733
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT22660.26387713060986356No Hit
CTGTAGGACGTGGAATATGGCAAGA22150.2579381484116716No Hit
GTCCTACAGTGTGCATTTCTCATTT18010.20972758703811314No Hit
GTATCAACGCAGAGTACTTTTTTTT15810.1841084481439516No Hit
CTGTAGGACCTGGAATATGGCGAGA15780.1837590962499403No Hit
CTTTAGGACGTGAAATATGGCGAGG15780.1837590962499403No Hit
CCCATGTACTCTGCGTTGATACCAC15410.1794504228904677No Hit
GTCCTAAAGTGTGTATTTCTCATTT15160.17653915710704027No Hit
GTACATGGGAAGCAGTGGTATCAAC15040.17514174953099507No Hit
GGTATCAACGCAGAGTACTTTTTTT10180.11854674270116555No Hit
GATATACACTGTTCTACAAATCCCG9240.10760038335547836No Hit
CTGAAGGACCTGGAATATGGCGAGA8790.10236010494530896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5800.015.2948244
CCAACGA852.7946407E-713.37152119
CCCTTAG751.42311255E-512.7123131
GTGCACC604.160823E-412.64043711
TCGAACT604.193671E-412.62865919
ACTGTTC4300.012.5948438
AGGACCT8650.012.5712445
AGGACGT9350.012.344215
CTTAGCC701.04689694E-412.266933
GTCCTAC11450.012.2404581
GGACGTG9300.012.2054436
TGTAGGA13450.012.2010612
GGACCTG8150.012.1720936
CTGTAGG13250.012.0886891
GACGTGG5200.012.0594357
GTAGGAC13250.012.0251913
GTCCTAA4700.011.9685071
GTATAGG802.7593349E-511.9177931
TTTAGGA5900.011.8048982
GTCCTAT1052.59035E-711.804291