FastQCFastQC Report
Thu 26 May 2016
SRR1926707_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926707_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736062
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGCG507.463958E-414.5836385
GAGTAGG550.004652014514.5205791
GTCTTAC550.004652014514.5205791
TGCAGGG700.001224692213.6579462
TCTATAC806.781538E-613.6576133
CTATAAT904.8624846E-513.3105311
GTATAGG700.001487480713.3105311
ACGCACA450.00967988713.00031958
GTCCTAC4150.012.8294271
GTCTTAG854.4792867E-412.5275591
TAATACA600.0024034612.4887754
GTCCTAA2350.012.4609221
TAGGACC2000.012.3103654
GATATAC1204.2708034E-612.2013211
TCTTAGA905.8339996E-412.1403962
TGTAGGA4400.012.10590652
AGGACAC706.883772E-411.9050095
GAACAGC706.8945397E-411.9027956
GCTTAAA907.245106E-411.8315831
GTGTAGG907.245106E-411.8315831
Produced by FastQC (version 0.11.5)