##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926707_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 736062 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.535234803589915 27.0 10.0 31.0 2.0 31.0 2 16.565491765639308 20.0 2.0 30.0 2.0 31.0 3 20.227589523708602 25.0 15.0 31.0 2.0 31.0 4 22.28745812173431 28.0 7.0 35.0 2.0 37.0 5 23.139295331099827 30.0 7.0 35.0 2.0 37.0 6 23.52958854009581 30.0 2.0 35.0 2.0 37.0 7 23.49311471044559 30.0 7.0 35.0 2.0 37.0 8 23.60346818610389 31.0 10.0 35.0 2.0 37.0 9 26.48511402572066 32.0 17.0 37.0 2.0 39.0 10 27.263261790447 32.0 24.0 37.0 2.0 39.0 11 24.319358967043538 30.0 8.0 37.0 2.0 39.0 12 25.52554540242534 31.0 16.0 37.0 2.0 39.0 13 22.122859215663897 28.0 2.0 37.0 2.0 39.0 14 25.515993217962617 31.0 17.0 38.0 2.0 40.0 15 26.61927391986001 31.0 17.0 38.0 2.0 40.0 16 26.990973586464182 32.0 18.0 39.0 2.0 40.0 17 26.315159592534325 31.0 17.0 38.0 2.0 40.0 18 26.381389067768747 31.0 17.0 38.0 2.0 40.0 19 26.793575812907065 31.0 18.0 39.0 2.0 40.0 20 27.03450796264445 31.0 19.0 39.0 2.0 40.0 21 24.859475152908313 30.0 8.0 38.0 2.0 40.0 22 25.8044526140461 31.0 10.0 38.0 2.0 40.0 23 25.795464240784064 31.0 2.0 38.0 2.0 40.0 24 24.82947632128815 31.0 2.0 39.0 2.0 40.0 25 24.633793077213603 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.81158154842046 1101 2 10.669406458244877 1101 3 7.171850849578959 1101 4 8.540824940981235 1101 5 8.352048229966666 1101 6 7.553279689135891 1101 7 7.313315908532864 1101 8 5.74757012736481 1101 9 5.772054906659431 1101 10 5.548585666721511 1101 11 8.186967530108966 1101 12 5.993787923596095 1101 13 10.489626340342372 1101 14 7.688020818254465 1101 15 6.919806759527933 1101 16 6.580787214098397 1101 17 2.614936789318641 1101 18 4.979526069339126 1101 19 5.5005978415524375 1101 20 3.66069736802476 1101 21 1.1142567012866316 1101 22 4.825922527565588 1101 23 6.107590625374662 1101 24 7.269617122288871 1101 25 7.655559273032829 1102 1 6.018728689167659 1102 2 10.989883520782668 1102 3 7.312379357850919 1102 4 8.342422459889278 1102 5 8.55370571933554 1102 6 7.071790612680989 1102 7 7.15706312064987 1102 8 7.206454428981537 1102 9 6.423695297012614 1102 10 5.716607657542564 1102 11 8.634415935687702 1102 12 5.755511900876705 1102 13 10.479643241108604 1102 14 7.778107230236664 1102 15 5.889469636325806 1102 16 6.357015617298142 1102 17 7.208104741302623 1102 18 7.261497511504803 1102 19 5.788739178546777 1102 20 6.187809839898122 1102 21 4.865827828235673 1102 22 6.371321653285481 1102 23 6.8451002900357025 1102 24 8.220829169908388 1102 25 7.86319150542084 1103 1 5.07078280135703 1103 2 9.302922511494684 1103 3 6.32533557941451 1103 4 7.551788135969488 1103 5 7.538422544766981 1103 6 6.676224090075593 1103 7 6.993817083160899 1103 8 6.229167621491673 1103 9 5.299071351451911 1103 10 4.595570788099742 1103 11 5.377257271690798 1103 12 4.1934746894535735 1103 13 8.60160754629382 1103 14 6.655914318646008 1103 15 5.615969641282042 1103 16 5.352909375413976 1103 17 3.26802997647604 1103 18 1.9740444718449979 1103 19 4.290730966063009 1103 20 4.601965966301975 1103 21 6.753254352030563 1103 22 2.3122185338302685 1103 23 4.726228056854694 1103 24 6.350274913988166 1103 25 2.430030690417322 1104 1 5.73465777376763 1104 2 10.205165642868588 1104 3 6.923421706709721 1104 4 7.101386464581129 1104 5 7.700541773291846 1104 6 7.059002259228077 1104 7 7.1850749480287845 1104 8 6.826718590068481 1104 9 6.107182785405165 1104 10 4.69014572997304 1104 11 7.876522259914282 1104 12 5.238293778595292 1104 13 9.360082857940625 1104 14 7.071112826248591 1104 15 6.296238611449208 1104 16 5.731975296688201 1104 17 6.268530062645663 1104 18 4.081610164845305 1104 19 4.878097592114049 1104 20 4.020432267931941 1104 21 3.4051538631313285 1104 22 5.270774270459615 1104 23 5.620936637232813 1104 24 6.775588223718078 1104 25 5.803759537286343 1105 1 3.5715961900163933 1105 2 9.545720976527372 1105 3 6.49913732380465 1105 4 5.944058693460256 1105 5 2.4307360069343567 1105 6 4.851768424408036 1105 7 5.9047157711178855 1105 8 3.201632420154727 1105 9 4.16310373098128 1105 10 3.7751913667428134 1105 11 6.948706759684988 1105 12 4.42102429236029 1105 13 5.226397448393147 1105 14 3.8719678684829404 1105 15 4.528040857044559 1105 16 4.615017945248088 1105 17 5.463778513066529 1105 18 2.916246771895704 1105 19 2.650029129562828 1105 20 1.7059927052165165 1105 21 3.0990132397493255 1105 22 4.182258168150412 1105 23 1.8821298501501644 1105 24 4.823917341012709 1105 25 5.468319663915434 1106 1 6.303412665984936 1106 2 10.891220588149045 1106 3 7.451500322259864 1106 4 8.805192059958479 1106 5 8.465604525598334 1106 6 7.376718022501926 1106 7 7.296152247260981 1106 8 7.098531502883674 1106 9 6.028936038821286 1106 10 5.367568678466601 1106 11 7.661823473917092 1106 12 4.874299325306989 1106 13 4.447849896049352 1106 14 5.545110416419298 1106 15 5.544001897025709 1106 16 5.490832885158543 1106 17 6.176319205514535 1106 18 6.05309209259147 1106 19 5.968769911032382 1106 20 5.350913676773256 1106 21 5.735047766145957 1106 22 6.091480626017908 1106 23 6.487212806014675 1106 24 7.24419330781291 1106 25 6.43682872753093 1107 1 4.201666272666792 1107 2 -13.952363314660369 1107 3 -5.562167103708246 1107 4 1.9593302528210472 1107 5 4.720729810336376 1107 6 4.996307999722884 1107 7 4.90738990861254 1107 8 5.502479870385571 1107 9 3.728252667522881 1107 10 3.0790341302509603 1107 11 5.995156807250424 1107 12 3.471869560690436 1107 13 5.108442151174636 1107 14 4.289985131681259 1107 15 3.9517467717409716 1107 16 3.6890105616961293 1107 17 4.276546995947335 1107 18 -0.8709777631412585 1107 19 1.8153910196124876 1107 20 2.275742833948655 1107 21 -1.3909962733377164 1107 22 2.270675766488676 1107 23 -1.157434118814482 1107 24 2.6003056844264236 1107 25 3.441384536186966 1108 1 5.327712580414669 1108 2 10.684679254421036 1108 3 0.426751858583561 1108 4 6.1557732743077445 1108 5 7.484093147134484 1108 6 7.48907195337544 1108 7 7.692601166234617 1108 8 7.522726963746759 1108 9 5.998070066583708 1108 10 5.069781313492118 1108 11 7.445964677900392 1108 12 2.7754398652559438 1108 13 3.955436389216487 1108 14 5.238035643656843 1108 15 5.318351004435392 1108 16 5.273297841894241 1108 17 0.29849018386791926 1108 18 4.127826294556581 1108 19 5.008665843070652 1108 20 5.320273963890141 1108 21 4.948051951660485 1108 22 2.3198710147019916 1108 23 5.084544873656583 1108 24 5.979700091907613 1108 25 6.544240677767483 1109 1 3.7587661021548833 1109 2 -13.952363314660369 1109 3 -5.560904208519522 1109 4 2.3233611447174596 1109 5 2.1400176313097 1109 6 3.2094400640240437 1109 7 4.2378220125032655 1109 8 4.216998772900169 1109 9 3.6413162623278623 1109 10 2.9161766057661964 1109 11 1.197550351623832 1109 12 1.6614879485406533 1109 13 2.872457038777565 1109 14 3.7752473433783535 1109 15 3.6540386347008997 1109 16 3.566663754673012 1109 17 4.081257913883817 1109 18 4.034333977632507 1109 19 2.5535150516836502 1109 20 1.5676858153439674 1109 21 0.7718690727942388 1109 22 2.846998196445824 1109 23 3.5077602731848145 1109 24 4.4592416756758055 1109 25 4.7283806265667 1110 1 3.189840108857588 1110 2 9.14463437587081 1110 3 6.387412124048868 1110 4 7.475578076186135 1110 5 8.53623183484796 1110 6 4.12568735408869 1110 7 6.8687181425264825 1110 8 7.543350629564262 1110 9 5.773072495636676 1110 10 5.270895400791993 1110 11 3.2173077170969684 1110 12 6.01919087496583 1110 13 10.433998343865351 1110 14 8.083465660772326 1110 15 7.228186857947112 1110 16 6.743020918357377 1110 17 7.166081787911015 1110 18 7.472742042351317 1110 19 6.317934591766296 1110 20 6.56921021116716 1110 21 3.9363275310255474 1110 22 6.349907907560922 1110 23 6.947605503393596 1110 24 8.008865975240628 1110 25 8.29452195922659 1111 1 5.285650689246527 1111 2 9.804646031134025 1111 3 7.760072977473143 1111 4 8.318710464148118 1111 5 8.762228827502266 1111 6 8.003148808982104 1111 7 7.676395507573645 1111 8 8.105932917880942 1111 9 6.628230669069872 1111 10 5.8181963440049635 1111 11 7.495197610832623 1111 12 7.05113672835413 1111 13 8.483295790499355 1111 14 6.750920136897022 1111 15 6.115434776907062 1111 16 6.033994204781873 1111 17 7.271038512350376 1111 18 7.045612618567386 1111 19 5.832764475285607 1111 20 5.359901109269941 1111 21 8.16081185757437 1111 22 7.586245349014806 1111 23 7.571136904255511 1111 24 7.212444572723957 1111 25 8.017641109111839 1112 1 -2.182712500745499 1112 2 7.666945947506035 1112 3 5.803289329434488 1112 4 -0.2594010104920592 1112 5 3.900936359400717 1112 6 2.6639664070493687 1112 7 5.549850712804549 1112 8 5.766474201252663 1112 9 4.251647972727493 1112 10 2.966780292321886 1112 11 -0.22851825941194903 1112 12 3.6819570651157214 1112 13 7.729521527379298 1112 14 5.380184063891257 1112 15 3.1018476742011316 1112 16 3.6805009023566626 1112 17 4.591466971296857 1112 18 4.546742329977469 1112 19 4.074660874391618 1112 20 3.965661170303701 1112 21 -2.356401569852679 1112 22 2.1087478798617987 1112 23 3.475096762821508 1112 24 5.3272790039802835 1112 25 5.445339578866385 1113 1 -18.94487890576905 1113 2 -13.952363314660369 1113 3 -7.876394248831424 1113 4 -6.470430499602246 1113 5 -2.1419351638415804 1113 6 -1.4058783247364772 1113 7 -20.8017976388438 1113 8 -10.025058814380678 1113 9 -5.803530763476324 1113 10 -5.109193412877556 1113 11 -6.105436143418167 1113 12 -4.266519213240599 1113 13 -2.722589127021436 1113 14 -3.2955908457985927 1113 15 -3.6947897124035407 1113 16 -3.8813407456354767 1113 17 -3.790563272783551 1113 18 -4.508151461557542 1113 19 -4.142343132143466 1113 20 -5.189079097304617 1113 21 -2.2783433745643826 1113 22 -3.280577080891735 1113 23 -3.673332113524488 1113 24 -3.3625001372710486 1113 25 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2204 9 2.732592039786379 2204 10 2.2488817022604124 2204 11 3.877596364726706 2204 12 3.984158535207712 2204 13 2.206816039720536 2204 14 3.328022940635339 2204 15 3.339733842327618 2204 16 3.3439630521338977 2204 17 -1.7694599300172271 2204 18 1.704491123240281 2204 19 2.247873509553518 2204 20 2.6309291466531164 2204 21 4.252712169100846 2204 22 3.754469648080228 2204 23 2.559454661264855 2204 24 4.251385382692806 2204 25 4.269198741510593 2205 1 5.864791086556355 2205 2 10.459762548731804 2205 3 7.213097151258534 2205 4 7.563512179815056 2205 5 8.107389647369192 2205 6 7.5565063854351955 2205 7 7.718540043427879 2205 8 7.125882088618013 2205 9 6.029655131668761 2205 10 5.498117828812365 2205 11 7.88600853601606 2205 12 7.054694896301093 2205 13 10.082287334097522 2205 14 7.798735745736437 2205 15 6.46907172303904 2205 16 6.391757741121449 2205 17 6.83479205975954 2205 18 7.057174721837338 2205 19 5.5217633826716295 2205 20 5.591211313615055 2205 21 -0.5764354007296184 2205 22 3.943521153950936 2205 23 5.617625596776723 2205 24 7.167802804267268 2205 25 7.628779316412029 2206 1 4.25375642402625 2206 2 8.83914540406594 2206 3 6.211777516217918 2206 4 1.9303852673479405 2206 5 5.868372205261778 2206 6 6.181628210208842 2206 7 6.609119722793803 2206 8 6.2819347506675065 2206 9 5.07150757808477 2206 10 4.131664254888076 2206 11 3.9493667279065896 2206 12 4.880683444335983 2206 13 8.185782097741459 2206 14 0.6452432222137077 2206 15 2.6643998193374863 2206 16 3.592543587279234 2206 17 4.6442072019315965 2206 18 -0.656462728799994 2206 19 1.217816754577715 2206 20 2.9242800993006206 2206 21 3.602248304046846 2206 22 -1.7248530475331307 2206 23 -3.0007031331560086 2206 24 1.4368215788104166 2206 25 3.085793135923428 2207 1 1.5585799720172702 2207 2 -13.952363314660369 2207 3 -6.1891183946082435 2207 4 -4.461555614425514 2207 5 1.473287418904519 2207 6 2.1911589904624407 2207 7 3.4510847593114633 2207 8 3.5833346768921004 2207 9 0.8519837849332959 2207 10 0.7390240801580745 2207 11 -1.484338260208343 2207 12 -2.18439800289741 2207 13 0.5180569734858409 2207 14 -5.192477106057716 2207 15 -1.2049903214476707 2207 16 -0.19344945825231363 2207 17 1.287653457480051 2207 18 1.090157792301465 2207 19 0.5422193398637773 2207 20 0.35924381473733646 2207 21 -3.801232076997888 2207 22 -4.175273468286047 2207 23 -1.186508185431073 2207 24 0.5358047485771849 2207 25 1.1717461469332129 2208 1 5.004273636603834 2208 2 6.7145489918982175 2208 3 6.5746487206486 2208 4 8.386324566173617 2208 5 3.3919209511853943 2208 6 6.377846063554564 2208 7 6.817951808466443 2208 8 6.943909304311518 2208 9 5.261830074024449 2208 10 4.8950862215752835 2208 11 6.970144063631885 2208 12 6.533409907462342 2208 13 8.778469999688124 2208 14 3.0989080706982186 2208 15 4.644294594582572 2208 16 5.266903866694147 2208 17 6.190236188183771 2208 18 0.7641781734265756 2208 19 3.5736746514346613 2208 20 4.694617601807767 2208 21 7.440095805810927 2208 22 6.316327856470092 2208 23 2.1723841580244745 2208 24 3.5283007425322914 2208 25 5.236888486848599 2209 1 2.895371681544969 2209 2 4.8612623705393165 2209 3 5.320637067276689 2209 4 0.9738711430172557 2209 5 5.081883782041842 2209 6 5.673874834006682 2209 7 6.097967435549307 2209 8 4.504813666910621 2209 9 4.4572545934252865 2209 10 3.3387187912320826 2209 11 -0.3893832921306597 2209 12 3.3641872971278097 2209 13 2.3494540615639856 2209 14 3.8587334259287047 2209 15 4.143769171430829 2209 16 3.8736611373560272 2209 17 1.5992750821925625 2209 18 3.5566599769350518 2209 19 4.006470981724714 2209 20 4.0788649482910095 2209 21 6.28105388217935 2209 22 5.084340695145851 2209 23 0.3707073050927505 2209 24 -3.1508997140462895 2209 25 0.051800463322567936 2210 1 -2.8773891938348903 2210 2 -13.952363314660369 2210 3 -8.025937175982495 2210 4 -2.961574909491354 2210 5 -0.867546950352434 2210 6 -1.7142312917832534 2210 7 -1.6199046347285737 2210 8 -20.947026583676692 2210 9 -13.475380730491686 2210 10 -7.6924686304191 2210 11 -10.490231795728661 2210 12 -5.265168413755788 2210 13 -5.575163499527953 2210 14 -4.655575767106672 2210 15 -6.368794675806299 2210 16 -5.760177173242049 2210 17 -5.186115058666125 2210 18 -5.60119327351369 2210 19 -11.101297676170107 2210 20 -8.053848361904244 2210 21 -5.934674854475169 2210 22 -11.06247008500696 2210 23 -8.96166183425455 2210 24 -11.335980487850636 2210 25 -9.133695377946351 2211 1 0.09089345195452836 2211 2 5.068774896721743 2211 3 0.8014642091424662 2211 4 1.5814944471281365 2211 5 1.2526778815255142 2211 6 0.42474291277360976 2211 7 -20.8017976388438 2211 8 -12.875481868229537 2211 9 -8.798385849431764 2211 10 -6.367496232546973 2211 11 -9.503400520008498 2211 12 -4.364154662877539 2211 13 -6.21757040507849 2211 14 -4.196978958916368 2211 15 -4.6039748424227795 2211 16 -11.204942806083562 2211 17 -7.222278864748631 2211 18 -5.221076842719754 2211 19 -7.899470917161775 2211 20 -6.100109528219612 2211 21 -3.8266852930980733 2211 22 -5.460251877639703 2211 23 -7.524243385995991 2211 24 -22.217461969332117 2211 25 -22.071143937617133 2212 1 -1.2712622257369723 2212 2 -13.952363314660369 2212 3 -8.007358265609946 2212 4 -19.629887666693 2212 5 -20.409110736360663 2212 6 -20.785013184787367 2212 7 -10.112944391049094 2212 8 -9.723289614951753 2212 9 -5.716304626855305 2212 10 -6.053820483921001 2212 11 -6.089928186407857 2212 12 -3.3532250170935107 2212 13 -6.595165413586715 2212 14 -4.250904803708096 2212 15 -4.336647728895027 2212 16 -4.341945433527638 2212 17 -4.234305778121211 2212 18 -3.6657544755425917 2212 19 -4.0466651589518285 2212 20 -4.061645181266599 2212 21 -2.049485379158277 2212 22 -3.55323145138091 2212 23 -3.5908631107337357 2212 24 -2.5470529877763823 2212 25 -3.136099831763083 2213 1 -18.94487890576905 2213 2 -13.952363314660369 2213 3 -8.594134706846692 2213 4 -5.725887666693001 2213 5 -20.409110736360663 2213 6 -10.508568740342923 2213 7 -20.8017976388438 2213 8 -10.547026583676692 2213 9 -8.126244928022551 2213 10 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2214 22 -12.915963200148822 2214 23 -12.909184854179001 2214 24 -11.746298723975324 2214 25 -11.503206498711952 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 126520.0 3 5.0 4 578.0 5 2321.0 6 3255.0 7 3054.0 8 2726.0 9 2501.0 10 2403.0 11 2219.0 12 2242.0 13 2420.0 14 3438.0 15 7606.0 16 10946.0 17 11644.0 18 11941.0 19 13850.0 20 16664.0 21 19312.0 22 20052.0 23 21847.0 24 23073.0 25 23657.0 26 19551.0 27 15387.0 28 16525.0 29 19051.0 30 18534.0 31 21647.0 32 27883.0 33 35089.0 34 43010.0 35 49230.0 36 49010.0 37 50561.0 38 36310.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55099716344369 19.087933245750627 12.516577783741445 25.84449180706424 2 13.679811761505059 21.02187826138709 41.43797545842748 23.860334518680375 3 18.211631334446114 25.243121493232817 30.044358001246852 26.500889171074217 4 11.749941282392546 17.324356063571596 39.2351053002427 31.69059735379316 5 12.223612064668428 37.82764490674993 37.21930146309655 12.729441565485091 6 30.28391717522869 37.941529111739854 19.311189210449523 12.46336450258193 7 26.76427977035028 31.349641097481395 23.239461915623487 18.646617216544836 8 23.96059756632027 37.434812150162216 21.7061936178411 16.89839666567641 9 26.222908321829074 15.418335634184988 20.686039157548265 37.67271688643767 10 14.09135591026866 29.454557469855963 35.50484204497581 20.949244574899566 11 32.69077978520886 22.351208649114614 25.842175209223804 19.115836356452714 12 23.245923757032955 24.8933574463199 32.401682167872316 19.459036628774832 13 27.954348547263457 21.767697426268104 25.72733450784745 24.550619518620987 14 20.17679567152572 22.50952782787586 28.51026688948823 28.803409611110194 15 22.21383468442588 31.59571508652444 23.933140140379425 22.257310088670256 16 21.306286161500402 28.302820247645133 28.43991950135562 21.95097408949884 17 20.94108573057383 28.51216458660526 29.155465261204665 21.391284421616245 18 20.856597717300712 27.68224054443136 30.89716832324234 20.56399341502559 19 22.510443429244905 27.123864440570898 27.822892657794227 22.54279947238997 20 22.86610872959855 27.8127358991898 28.430536289981934 20.890619081229715 21 21.985076256894892 26.536236385736633 27.046695912777942 24.431991444590533 22 22.332647522808255 28.288718876521006 28.14737387889285 21.23125972177789 23 21.564781667228868 28.044444565347494 28.567289800981495 21.82348396644215 24 22.31353284048481 27.361151319618337 28.662563906655247 21.66275193324161 25 22.179708126298152 27.58912431088399 28.612001670336966 21.6191658924809 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 670.0 1 670.0 2 1451.5 3 2233.0 4 2233.0 5 2233.0 6 3205.5 7 4178.0 8 4178.0 9 4178.0 10 4979.5 11 5781.0 12 5781.0 13 5781.0 14 7445.0 15 9109.0 16 9109.0 17 9109.0 18 13190.0 19 17271.0 20 17271.0 21 17271.0 22 24158.0 23 31045.0 24 31045.0 25 31045.0 26 40415.5 27 49786.0 28 49786.0 29 49786.0 30 59714.0 31 69642.0 32 69642.0 33 69642.0 34 76264.0 35 82886.0 36 82886.0 37 82886.0 38 86360.0 39 89834.0 40 89834.0 41 89834.0 42 90424.5 43 91015.0 44 91015.0 45 91015.0 46 88855.5 47 86696.0 48 86696.0 49 86696.0 50 79567.5 51 72439.0 52 72439.0 53 72439.0 54 62119.5 55 51800.0 56 51800.0 57 51800.0 58 43069.5 59 34339.0 60 34339.0 61 34339.0 62 27197.5 63 20056.0 64 20056.0 65 20056.0 66 15182.5 67 10309.0 68 10309.0 69 10309.0 70 7381.5 71 4454.0 72 4454.0 73 4454.0 74 3073.5 75 1693.0 76 1693.0 77 1693.0 78 1129.0 79 565.0 80 565.0 81 565.0 82 376.5 83 188.0 84 188.0 85 188.0 86 120.5 87 53.0 88 53.0 89 53.0 90 35.5 91 18.0 92 18.0 93 18.0 94 10.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.926085030880552 2 36.08323755335828 3 5.421825878798253 4 13.234211248508956 5 13.327681635514399 6 14.33656403944233 7 9.22666840565061 8 7.62218400080428 9 0.1123546657754374 10 1.3199975002105802 11 5.439351576361775 12 5.344930182511799 13 15.636726254038383 14 1.4697131491640651 15 2.814300969211833 16 2.7880803519268755 17 2.7685167825536436 18 1.381269512622578 19 1.3270621224842472 20 0.2945404055636618 21 4.213096179397931 22 1.4789515013680912 23 0.11602283503291842 24 8.959027907975143 25 0.11969100429039944 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 736062.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.38889730088262 #Duplication Level Percentage of deduplicated Percentage of total 1 96.9659399899399 90.55542211404044 2 2.2569186303015116 4.215422843633531 3 0.36097563582460146 1.0113334974643353 4 0.14491885840025315 0.5413524953640957 5 0.07465592380749776 0.34860172006804635 6 0.04358966180701858 0.24424742699255178 7 0.027471603811855566 0.17958799509531403 8 0.01888068278243708 0.14105969162716475 9 0.01426902521062856 0.1199311676981186 >10 0.08091051038209161 1.3146050135761174 >50 0.007001056625986327 0.45822452688005794 >100 0.004170526373108324 0.7077423534494388 >500 2.978947330942482E-4 0.16246915411080953 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3585812064744547E-4 2 0.0 0.0 0.0 0.0 1.3585812064744547E-4 3 0.0 0.0 0.0 0.0 1.3585812064744547E-4 4 0.0 0.0 0.0 0.0 1.3585812064744547E-4 5 0.0 0.0 0.0 0.0 1.3585812064744547E-4 6 0.0 0.0 0.0 0.0 1.3585812064744547E-4 7 0.0 0.0 0.0 0.0 1.3585812064744547E-4 8 0.0 0.0 0.0 0.0 1.3585812064744547E-4 9 0.0 0.0 0.0 0.0 1.3585812064744547E-4 10 0.0 0.0 0.0 0.0 1.3585812064744547E-4 11 0.0 0.0 0.0 1.3585812064744547E-4 1.3585812064744547E-4 12 0.0 0.0 0.0 1.3585812064744547E-4 1.3585812064744547E-4 13 0.0 0.0 0.0 1.3585812064744547E-4 1.3585812064744547E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAGGCG 50 7.463958E-4 14.583638 5 GAGTAGG 55 0.0046520145 14.520579 1 GTCTTAC 55 0.0046520145 14.520579 1 TGCAGGG 70 0.0012246922 13.657946 2 TCTATAC 80 6.781538E-6 13.657613 3 CTATAAT 90 4.8624846E-5 13.310531 1 GTATAGG 70 0.0014874807 13.310531 1 ACGCACA 45 0.009679887 13.0003195 8 GTCCTAC 415 0.0 12.829427 1 GTCTTAG 85 4.4792867E-4 12.527559 1 TAATACA 60 0.00240346 12.488775 4 GTCCTAA 235 0.0 12.460922 1 TAGGACC 200 0.0 12.310365 4 GATATAC 120 4.2708034E-6 12.201321 1 TCTTAGA 90 5.8339996E-4 12.140396 2 TGTAGGA 440 0.0 12.1059065 2 AGGACAC 70 6.883772E-4 11.905009 5 GAACAGC 70 6.8945397E-4 11.902795 6 GCTTAAA 90 7.245106E-4 11.831583 1 GTGTAGG 90 7.245106E-4 11.831583 1 >>END_MODULE