FastQCFastQC Report
Thu 26 May 2016
SRR1926707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926707_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736062
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11910.16180702169110753No Hit
GTCCTACAGTGGACATTTCTAAATT11610.1577312780716842No Hit
CTTTAGGACGTGAAATATGGCGAGG11610.1577312780716842No Hit
CTGTAGGACGTGGAATATGGCAAGA11550.1569161293477995No Hit
GTCCTAAAGTGTGTATTTCTCATTT11340.15406310881420315No Hit
GTCCTACAGTGTGCATTTCTCATTT8180.1111319426896104No Hit
GGTATCAACGCAGAGTACTTTTTTT7740.10515418538112278No Hit
CTGTAGGACCTGGAATATGGCGAGA7390.1003991511584622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTAC508.3936524E-515.264811
TAGGACC3050.014.0771214
GGCGAGG3150.013.23046319
TCTAGGA1102.5538611E-913.0142342
CAAGAGT550.002968087312.1432934
TAGTCCA550.002968087312.1432935
GGACCTG4850.011.99946
AATACTG802.7376445E-511.9264495
TTAGGAC5000.011.8318463
AGGACCT5550.011.6900695
TGGCGAG7150.011.65761118
ACGTGAA4550.011.4845468
TAGAGTG1001.8272367E-611.449395
CCCCTAT751.990699E-411.4486071
GTATTGA751.990699E-411.4486071
GTCCTAA3250.011.4486071
GACGTGA4250.011.4010227
GTCCTAC6550.011.2155571
CGTGAAA4750.011.2010049
TCCAACG855.4770942E-511.14330518
Produced by FastQC (version 0.11.5)