FastQCFastQC Report
Thu 26 May 2016
SRR1926706_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926706_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50116
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT640.1277037273525421No Hit
CTGTAGGACGTGGAATATGGCAAGA580.11573150291324127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTACA459.605271E-418.2991452
TGTAGGA601.6750115E-518.2991452
CCTACAG400.001893349716.5541233
GTCCTAC550.004302005314.6238411
CTACAGT450.004758652314.4175084
CTGTAGG700.001353471313.4051891
GTAGGAC754.835187E-513.2432983
TGTGCAT550.003772749311.71643910
TTTCTAA500.004635140811.403151516
GTGTGCA600.007216727410.7400699
TGGAATA700.001869448310.520884511
ATATGGC750.0091218378.68811515
AATATGG1001.7177437E-48.68582614
Produced by FastQC (version 0.11.5)