FastQCFastQC Report
Thu 26 May 2016
SRR1926706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926706_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50116
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT1590.31726394764147176No Hit
GTCCTAAAGTGTGTATTTCTCATTT1410.2813472743235693No Hit
CTTTAGGACGTGAAATATGGCGAGG1400.27935190358368583No Hit
CTGTAGGACGTGGAATATGGCAAGA1180.2354537473062495No Hit
GTCCTACAGTGTGCATTTCTCATTT1180.2354537473062495No Hit
CTGAAGGACCTGGAATATGGCGAGA860.17160188362997847No Hit
GTATCAACGCAGAGTACTTTTTTTT860.17160188362997847No Hit
GTACATGGGAAGCAGTGGTATCAAC700.13967595179184292No Hit
CTGTAGGACCTGGAATATGGCGAGA680.13568521031207598No Hit
CCCATGTACTCTGCGTTGATACCAC680.13568521031207598No Hit
GGTATCAACGCAGAGTACTTTTTTT510.10176390773405698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCAAG307.77959E-418.92714518
GGCAAGA250.006086327618.92714519
GGCGAGG350.002186442516.22326719
ATGGCAA456.797394E-414.72111417
GTAGGAC551.8034126E-413.9039593
TATGGCA500.001507566213.249001516
TGTAGGA603.7352843E-412.7581572
CAGTGTG701.0491536E-412.2162187
GTCCTAC751.9039364E-411.4592151
ATTTCTC752.0711956E-411.35628815
CTACAGT854.7751753E-511.245854
TACAGTG854.7751753E-511.245855
ACAGTGT700.001374906410.9245396
AGGACGT700.001374906410.9245395
GGACGTG700.001374906410.9245396
CCTACAG803.4318405E-410.7538433
ATCAACG908.542107E-510.6210793
GTATCAA908.630325E-510.6103841
ATATGGC1409.305222E-810.13954215
TCCTACA855.949058E-410.1314772
Produced by FastQC (version 0.11.5)