FastQCFastQC Report
Thu 26 May 2016
SRR1926705_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926705_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767663
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12850.16739115992303913No Hit
GTCCTAAAGTGTGTATTTCTCATTT11590.15097770766599405No Hit
CTGTAGGACGTGGAATATGGCAAGA10020.13052602509173947No Hit
CTTTAGGACGTGAAATATGGCGAGG9380.12218903346911339No Hit
GTCCTACAGTGTGCATTTCTCATTT9060.11802053765780038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAC851.5033766E-615.7897371
CTAAGAC701.5536316E-615.7415353
CTATAGG1159.637006E-915.1718771
TGTAAGA1106.7093424E-815.0227792
TACTATA656.5690366E-414.830182
GTCCTAA4450.014.4768831
ATGTAAG657.9829106E-414.4536821
GTGCTAT657.9829106E-414.4536821
TATAGGA1252.0603693E-814.3217162
GTAAGAC702.7061942E-514.1673813
TAGGACC3050.014.13235954
ATACAGC450.00600546313.9733256
GTCCTAC9150.013.49461651
GTCTAGA600.00783825313.4212761
TAGGCAG550.001459385713.3454575
TGTCGAG701.6159047E-613.30967718
TGTAGGA7450.013.308772
TAAGACC652.769564E-413.2626754
TCCTACA10000.013.2200452
TTAGGAC4850.013.1774493
Produced by FastQC (version 0.11.5)