##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926705_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 767663 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.527635173246594 27.0 7.0 31.0 2.0 31.0 2 16.50028984072438 18.0 2.0 31.0 2.0 31.0 3 20.21602057152683 25.0 15.0 31.0 2.0 31.0 4 22.217613718519715 28.0 7.0 35.0 2.0 37.0 5 23.062616017705686 30.0 2.0 35.0 2.0 37.0 6 23.514923866331973 30.0 2.0 35.0 2.0 37.0 7 23.46740171142806 30.0 7.0 35.0 2.0 37.0 8 23.671711415034984 31.0 10.0 35.0 2.0 37.0 9 26.5408284624894 32.0 17.0 37.0 2.0 39.0 10 27.300895054209985 32.0 25.0 38.0 2.0 39.0 11 24.319438347295623 30.0 8.0 37.0 2.0 39.0 12 25.51669677970672 31.0 16.0 37.0 2.0 39.0 13 22.034720964798357 28.0 2.0 37.0 2.0 39.0 14 25.49647566705703 31.0 17.0 38.0 2.0 40.0 15 26.646854153450146 31.0 17.0 38.0 2.0 40.0 16 27.029914168065936 32.0 18.0 39.0 2.0 40.0 17 26.37292014855477 31.0 17.0 38.0 2.0 40.0 18 26.48259718131524 31.0 17.0 38.0 2.0 40.0 19 26.88370417748413 31.0 18.0 39.0 2.0 40.0 20 27.133763122620213 31.0 21.0 39.0 2.0 40.0 21 24.976428458841966 30.0 8.0 38.0 2.0 40.0 22 25.914375188070807 31.0 10.0 38.0 2.0 40.0 23 25.9202762149537 31.0 2.0 38.0 2.0 40.0 24 24.915391258924814 31.0 2.0 39.0 2.0 40.0 25 24.720201442560082 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.202045549369533 1101 2 10.913319741685655 1101 3 7.4014054161949865 1101 4 8.744297277433073 1101 5 8.584992280270168 1101 6 8.230604197244563 1101 7 7.686840547180417 1101 8 6.215023385072563 1101 9 6.18621836485395 1101 10 5.5968158312947764 1101 11 8.608933671463287 1101 12 5.930052593004017 1101 13 10.690166888038373 1101 14 8.029577191508157 1101 15 7.149345363539318 1101 16 6.617839523873787 1101 17 2.8373390622914307 1101 18 5.392826043580147 1101 19 5.883767828888352 1101 20 3.704002974323302 1101 21 0.5720296298257423 1101 22 4.739576950325752 1101 23 5.979153310238679 1101 24 7.307572147011854 1101 25 7.7972705803180204 1102 1 5.661981966602934 1102 2 10.742690820566214 1102 3 6.843218204472407 1102 4 7.841288469075266 1102 5 7.970950662566722 1102 6 6.646814316960008 1102 7 6.629116233596026 1102 8 6.833452934457323 1102 9 5.666082738948905 1102 10 5.147460786945764 1102 11 7.7643746890238745 1102 12 5.174401956733245 1102 13 9.72756622172501 1102 14 7.162444022773958 1102 15 5.273630116495873 1102 16 5.553780565952909 1102 17 6.459900132646631 1102 18 6.226614003738412 1102 19 5.1415952975651855 1102 20 5.430495458948997 1102 21 4.570378321215365 1102 22 5.531899044716795 1102 23 5.812131272506129 1102 24 7.413654102728422 1102 25 7.018369282489893 1103 1 4.969151574000261 1103 2 9.350855010213238 1103 3 6.438041416144504 1103 4 7.6200132398109055 1103 5 7.287516109625482 1103 6 6.63618730657204 1103 7 6.56620550635456 1103 8 6.173523257970647 1103 9 5.126745486238981 1103 10 4.440916770046076 1103 11 5.352158959155311 1103 12 4.424789131469023 1103 13 8.483070094071476 1103 14 6.655600091487063 1103 15 5.72331597324991 1103 16 5.406762006129096 1103 17 3.219941063831076 1103 18 1.8491760011227 1103 19 3.7606051726927205 1103 20 4.331412276741123 1103 21 6.590126778956851 1103 22 2.489943382439808 1103 23 4.305331076237742 1103 24 5.965642757984444 1103 25 2.0003055216257835 1104 1 5.842493158966853 1104 2 10.555663515925563 1104 3 7.1281949618238265 1104 4 7.566744494801217 1104 5 7.73146763276419 1104 6 7.152495561470335 1104 7 7.251206233566844 1104 8 7.130046055333686 1104 9 5.906410849859967 1104 10 4.766368309509957 1104 11 7.831867955513314 1104 12 4.785222846811422 1104 13 9.344862954330956 1104 14 6.820833407901578 1104 15 6.025217707576999 1104 16 5.794360216890059 1104 17 5.945162863577593 1104 18 3.1811932286332905 1104 19 4.340647499415841 1104 20 3.5644431491287243 1104 21 2.884115849995105 1104 22 5.102757753341816 1104 23 5.4365534161990965 1104 24 6.640575305334867 1104 25 5.694127185428126 1105 1 3.34624227773903 1105 2 9.338547471222753 1105 3 6.030127884495361 1105 4 4.978995584056591 1105 5 2.148321331609637 1105 6 4.330807533455957 1105 7 5.422659074876492 1105 8 2.5939810120523994 1105 9 3.5841050955867324 1105 10 3.313635058617173 1105 11 6.313753956414768 1105 12 3.743423177344088 1105 13 4.914033271110689 1105 14 3.3831359261906684 1105 15 3.8007571345766635 1105 16 3.9208467471060615 1105 17 4.488431438704431 1105 18 1.9711385636154333 1105 19 1.8003970573572943 1105 20 0.5982915432715927 1105 21 2.257520105314253 1105 22 3.437283392737612 1105 23 0.9417853091342501 1105 24 4.013465270316896 1105 25 4.735826308245279 1106 1 6.37363885237092 1106 2 11.29427204032164 1106 3 7.820424191577246 1106 4 9.047261787212591 1106 5 8.621564055887525 1106 6 7.539344107723835 1106 7 7.621169349160752 1106 8 7.212610773805487 1106 9 6.250541220297798 1106 10 5.2209071405930345 1106 11 7.788006818471807 1106 12 5.4939236960634865 1106 13 4.643957920292571 1106 14 5.919480410474382 1106 15 5.97118148344731 1106 16 5.8030291907294504 1106 17 6.166547850258226 1106 18 6.3465594543567505 1106 19 6.055197486893356 1106 20 5.473450799227763 1106 21 5.974984113468157 1106 22 6.255415365873407 1106 23 6.515586794309794 1106 24 7.345056646834379 1106 25 6.545472214002157 1107 1 4.351665811746113 1107 2 -14.169051312971774 1107 3 -5.682140840839455 1107 4 2.0507732281416615 1107 5 5.048749198158372 1107 6 5.218731639897303 1107 7 5.143854407404678 1107 8 5.620443188739284 1107 9 4.022348418309452 1107 10 3.48787394553559 1107 11 6.050713679491274 1107 12 3.470358566352452 1107 13 4.871522731585788 1107 14 4.576087801305718 1107 15 4.304970699697233 1107 16 3.9182853843982457 1107 17 4.44126061085225 1107 18 -0.4051033942444242 1107 19 2.27579248596464 1107 20 2.726359678662398 1107 21 -0.9019993641851016 1107 22 2.3769039249443367 1107 23 -0.6952621871954356 1107 24 2.8689390887452397 1107 25 3.7178833002491594 1108 1 5.073392286678775 1108 2 10.469183981145871 1108 3 -0.06704939067002513 1108 4 5.678883837230202 1108 5 7.066082372196082 1108 6 7.084580106314888 1108 7 7.21623363235906 1108 8 6.997125567747016 1108 9 5.470420579226996 1108 10 4.674557193428544 1108 11 6.990168671236866 1108 12 2.308475474858838 1108 13 3.3046174835190563 1108 14 4.641094535166175 1108 15 5.068927168547699 1108 16 4.902839863501555 1108 17 0.13293125990950827 1108 18 3.5838128886646032 1108 19 4.774742220954646 1108 20 4.8846651352615815 1108 21 4.8172040852892195 1108 22 1.9223227276205002 1108 23 4.559959812978342 1108 24 5.364504515802324 1108 25 5.91502575398102 1109 1 3.2308789176948167 1109 2 -14.169051312971774 1109 3 -6.071376183020245 1109 4 1.7563582322270292 1109 5 1.6660971007377299 1109 6 2.566828814735082 1109 7 3.4940853046150195 1109 8 3.8379406225825115 1109 9 3.1149041738975285 1109 10 2.209009518272193 1109 11 1.0547080077727138 1109 12 1.1443462715596446 1109 13 2.386867324903996 1109 14 3.069807260626156 1109 15 3.030327059783083 1109 16 2.8245939195153333 1109 17 3.3319081927473597 1109 18 3.525063001961076 1109 19 1.9049640554511598 1109 20 0.932504571498324 1109 21 0.22787548019541148 1109 22 2.558838181259585 1109 23 3.0150989410486773 1109 24 4.075633628464338 1109 25 4.3791096386905295 1110 1 3.1849437350526877 1110 2 9.107383732345447 1110 3 6.216181016296389 1110 4 7.38902689747545 1110 5 8.221811446303068 1110 6 4.206065800290364 1110 7 6.485864430297571 1110 8 7.015125834318901 1110 9 5.087385658309987 1110 10 4.807023427656372 1110 11 2.5731502777767616 1110 12 5.473987737165572 1110 13 9.861670531324283 1110 14 7.366083428004277 1110 15 6.457611192006027 1110 16 6.118458754562514 1110 17 6.536154558292303 1110 18 6.679767660301351 1110 19 5.315174511695716 1110 20 5.756210807911302 1110 21 3.4646294451612683 1110 22 5.619465965580339 1110 23 6.207676180492115 1110 24 7.297303964887096 1110 25 7.712166770508475 1111 1 5.283313624795213 1111 2 9.855195564324333 1111 3 7.801531778020816 1111 4 8.47124996075529 1111 5 8.463029361866308 1111 6 7.859544449142394 1111 7 7.789096903310352 1111 8 7.783805452779649 1111 9 6.203177094764875 1111 10 5.370585740776946 1111 11 7.323014869966173 1111 12 6.728364073207803 1111 13 8.561096823969418 1111 14 6.475164963484453 1111 15 6.16339166264806 1111 16 5.677525972331569 1111 17 6.853543806912143 1111 18 6.913838929205724 1111 19 5.583409615171703 1111 20 5.356541891107199 1111 21 8.25546044955425 1111 22 7.711019403939815 1111 23 7.654568016289058 1111 24 7.373321021831629 1111 25 8.017637911996324 1112 1 -2.014589155898257 1112 2 7.653121306075846 1112 3 5.839034292803365 1112 4 -0.5983220451227389 1112 5 3.8912924562296958 1112 6 3.1094137897882774 1112 7 5.579573968493516 1112 8 5.7049074304921135 1112 9 4.275103702476294 1112 10 3.0237256107954877 1112 11 -0.2375536676987089 1112 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5.747465464696585 2204 5 0.767337512901225 2204 6 3.818120608140127 2204 7 5.038683496043735 2204 8 4.953602278619897 2204 9 3.3608363409755277 2204 10 3.2184282021619666 2204 11 4.752682533812532 2204 12 4.8687667044693015 2204 13 3.0493549032316416 2204 14 4.144018961024496 2204 15 4.301246608499643 2204 16 4.215284413198887 2204 17 -1.1039657180489968 2204 18 2.7256876865020416 2204 19 3.587090887836954 2204 20 3.999738953477003 2204 21 5.372882542846636 2204 22 4.842819700958149 2204 23 3.751418155938463 2204 24 5.575081083428589 2204 25 5.56153180730573 2205 1 6.118559072905462 2205 2 10.537503565077007 2205 3 7.2800004658579525 2205 4 7.961129174389455 2205 5 8.059410923128652 2205 6 7.520654353086769 2205 7 7.68192588486982 2205 8 7.374241780085612 2205 9 6.1460931043345965 2205 10 5.468647939483063 2205 11 7.772808556459246 2205 12 6.88204075462928 2205 13 10.12071325109438 2205 14 7.626496256830446 2205 15 6.300499980599348 2205 16 5.892402273831539 2205 17 6.612217486595306 2205 18 6.36968089440348 2205 19 5.16222428695178 2205 20 5.697344475290272 2205 21 -0.7951524426878223 2205 22 3.9833162713794685 2205 23 5.4185232993485 2205 24 7.1595009290017515 2205 25 7.622764276219186 2206 1 4.2477514289994005 2206 2 8.925232280198234 2206 3 6.286472051314341 2206 4 1.89838359704504 2206 5 6.025857362370328 2206 6 6.286923003384629 2206 7 6.624051113471772 2206 8 6.5615448655329445 2206 9 4.940605412625835 2206 10 4.233701151679995 2206 11 3.8973343771628883 2206 12 5.153249809982643 2206 13 8.586065909214504 2206 14 0.6327737569662517 2206 15 3.231243208549447 2206 16 4.032371174912534 2206 17 4.9222424743730215 2206 18 -0.6427281174928723 2206 19 1.5611177177012294 2206 20 3.1453069755166148 2206 21 3.7733511877827794 2206 22 -1.7260887750652039 2206 23 -2.9121284878208193 2206 24 1.7126927554634435 2206 25 3.140328714809005 2207 1 1.764184640066599 2207 2 -14.169051312971774 2207 3 -6.325193456952572 2207 4 -4.794648924393808 2207 5 1.4832967408592665 2207 6 2.1238828889313517 2207 7 3.32809604247117 2207 8 3.3578948071056303 2207 9 0.7368521974323627 2207 10 0.7203592614083547 2207 11 -1.3121533395720135 2207 12 -2.3248885655181333 2207 13 0.8004865486849866 2207 14 -5.67196824119722 2207 15 -1.4833473859210144 2207 16 -0.14572137192655887 2207 17 0.9101460752205988 2207 18 0.9960270340038306 2207 19 0.36271343449701376 2207 20 0.29040288594271857 2207 21 -3.6938644081673857 2207 22 -3.9857225178125226 2207 23 -1.1087993991347496 2207 24 0.6978618564606833 2207 25 1.2436731924552724 2208 1 5.527587943436103 2208 2 7.089013203157258 2208 3 6.806688222654877 2208 4 8.81749336706364 2208 5 4.238533879680798 2208 6 6.8045990816469555 2208 7 7.076693784161719 2208 8 7.0942555487716845 2208 9 5.35777299331091 2208 10 4.937152070088885 2208 11 6.912124711717574 2208 12 6.55893668084661 2208 13 9.21529374327027 2208 14 4.021700164514687 2208 15 5.369870664225541 2208 16 5.766763013406678 2208 17 6.5640270853364555 2208 18 1.2116196565330384 2208 19 3.902248546065337 2208 20 5.177030199566872 2208 21 7.817449183328435 2208 22 6.765739341577913 2208 23 2.7661689633051374 2208 24 4.340830153584317 2208 25 5.449742705277668 2209 1 3.4537286727302003 2209 2 5.148313956489304 2209 3 5.436442155649104 2209 4 0.7583396315239668 2209 5 5.19267184031866 2209 6 5.876170455029225 2209 7 6.3621873132326066 2209 8 4.711862270811476 2209 9 4.784223855028333 2209 10 3.821955044784655 2209 11 -0.1558554569772923 2209 12 3.5276063307940255 2209 13 2.4920118125081387 2209 14 4.201666039216896 2209 15 4.555762321770665 2209 16 4.179526196717475 2209 17 1.4711885688210984 2209 18 3.894463645973513 2209 19 4.433191498869405 2209 20 4.384378942940341 2209 21 6.62393620927654 2209 22 5.212002192220712 2209 23 1.2289101476032087 2209 24 -2.9401229938841844 2209 25 0.5843943696907772 2210 1 -2.1757482336299 2210 2 -14.169051312971774 2210 3 -7.758400876276049 2210 4 -2.1904764049027854 2210 5 -0.2790096984106363 2210 6 -1.137103272456887 2210 7 -1.1883392847889453 2210 8 -21.33155090284122 2210 9 -13.510334122496584 2210 10 -7.7140148457563384 2210 11 -10.500263263516612 2210 12 -5.1225922900318785 2210 13 -5.655279817899546 2210 14 -4.367371455314856 2210 15 -6.053034616952047 2210 16 -5.499562405339997 2210 17 -5.056832960782696 2210 18 -5.174547977284291 2210 19 -10.564519481369643 2210 20 -7.27127440080422 2210 21 -5.578413607978188 2210 22 -10.327643771154971 2210 23 -8.240726384517153 2210 24 -10.904887494733233 2210 25 -8.493456409504955 2211 1 -0.20258925347827272 2211 2 4.873883893897858 2211 3 0.801039074224132 2211 4 1.5599039501934193 2211 5 0.8971960471742726 2211 6 0.0405588912714947 2211 7 -21.116854602935057 2211 8 -13.314376820093365 2211 9 -8.966150688624406 2211 10 -7.107873237760327 2211 11 -9.902329721562877 2211 12 -4.558036785783123 2211 13 -6.968920538247026 2211 14 -4.815125903829095 2211 15 -5.275903386625188 2211 16 -11.917203645705406 2211 17 -7.8503165474101415 2211 18 -5.861987152663403 2211 19 -8.737443226995023 2211 20 -6.706839089379095 2211 21 -4.64640717888858 2211 22 -6.152523440354884 2211 23 -8.28639413843829 2211 24 -22.636230778315323 2211 25 -22.42982718719545 2212 1 -1.3037874741144293 2212 2 -14.169051312971774 2212 3 -8.042969203039416 2212 4 -19.91082204512274 2212 5 -20.689064686627447 2212 6 -21.188949305449817 2212 7 -10.080573423796734 2212 8 -9.696630267920584 2212 9 -5.465648848544113 2212 10 -5.718420553225673 2212 11 -5.440017292037332 2212 12 -2.902658423144807 2212 13 -6.523447631415804 2212 14 -4.158062349810038 2212 15 -4.06557663297291 2212 16 -4.267128679471188 2212 17 -4.531381687491674 2212 18 -3.497911467744629 2212 19 -4.033318225446134 2212 20 -4.266731534517444 2212 21 -2.152770015462192 2212 22 -3.5987645961979133 2212 23 -3.8090388976170075 2212 24 -2.7639707631981665 2212 25 -3.32627465507149 2213 1 -19.223666536850637 2213 2 -14.169051312971774 2213 3 -8.620386494742421 2213 4 -5.712786038576095 2213 5 -20.689064686627447 2213 6 -10.716772546039016 2213 7 -21.116854602935057 2213 8 -10.671976434756113 2213 9 -8.280694997936767 2213 10 -7.016678245100387 2213 11 -6.4816049400079585 2213 12 -5.043109438975762 2213 13 -19.627000163539766 2213 14 -12.477923227421677 2213 15 -6.848529748752771 2213 16 -6.102884840455314 2213 17 -4.634668865246937 2213 18 -4.702464429136494 2213 19 -5.667395788095128 2213 20 -5.609564430541827 2213 21 -3.781350598450068 2213 22 -4.598489345162786 2213 23 -4.650452240550109 2213 24 -3.614954182570642 2213 25 -4.3889106569172185 2214 1 -19.223666536850637 2214 2 -14.169051312971774 2214 3 -12.476521709009123 2214 4 -19.91082204512274 2214 5 -20.689064686627447 2214 6 -21.188949305449817 2214 7 -16.78467467213921 2214 8 -15.093661629484817 2214 9 -14.028238590323177 2214 10 -13.940624467471112 2214 11 -14.76653548101227 2214 12 -12.514664663549466 2214 13 -19.627000163539766 2214 14 -16.98749543023175 2214 15 -24.364896360864066 2214 16 -18.597981935806402 2214 17 -24.113392269502256 2214 18 -18.0673549314046 2214 19 -15.095197225412134 2214 20 -13.798509605222849 2214 21 -11.256541589451263 2214 22 -12.30769457341756 2214 23 -12.267375827160066 2214 24 -11.12152489596238 2214 25 -10.835536529756004 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 131756.0 3 5.0 4 557.0 5 2346.0 6 3218.0 7 3194.0 8 2789.0 9 2586.0 10 2386.0 11 2211.0 12 2172.0 13 2292.0 14 3511.0 15 7864.0 16 12150.0 17 12635.0 18 13178.0 19 15156.0 20 17600.0 21 20605.0 22 21052.0 23 22668.0 24 23591.0 25 24566.0 26 20128.0 27 15735.0 28 16401.0 29 19338.0 30 18969.0 31 21614.0 32 28248.0 33 35610.0 34 44194.0 35 51027.0 36 50991.0 37 53681.0 38 41639.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.020456838128425 19.358375794203543 12.830064262239544 25.791103105428487 2 12.609478901184657 20.84229227027072 43.79425853209029 22.753970296454337 3 18.14923445884255 25.734679824932076 30.209746223556582 25.90633949266879 4 11.283291654709684 17.595667245156854 39.94284942097839 31.17819167915507 5 11.251777100015795 38.76827223349576 37.89931589243836 12.080634774050086 6 29.434839974598166 38.94311098506101 19.722953518339263 11.899095522001565 7 25.97070736752344 31.544892514937285 23.796374719835907 18.688025397703374 8 22.77503937920834 39.89248132845795 21.74411008645044 15.588369205883268 9 26.27878772071673 15.154403901828331 20.819488276257793 37.747320101197154 10 13.851319173254595 29.924675365907866 36.21773502238309 20.006270438454457 11 32.38327754007139 21.927721861351895 26.998026913782784 18.690973684793935 12 23.569177552530704 24.55673537718722 33.8126993893938 18.061387680888274 13 28.557658398965557 22.37984404999804 25.81560283687943 23.24689471415697 14 19.05693723867111 23.74151426345662 28.623495620008587 28.578052877863687 15 21.205348738388384 34.307848294632436 23.620725823368996 20.866077143610184 16 19.86298422193651 28.27987906891673 31.144192026510158 20.7129446826366 17 19.847256409463597 29.44165643850128 30.51080779876886 20.200279353266268 18 19.75185421004629 27.560252725884016 33.55911696845427 19.12877609561542 19 22.645500825536597 26.737463485881207 27.911868676177974 22.705167012404218 20 22.700011626494298 28.647420681835108 29.405363602279838 19.24720408939076 21 21.080744442426173 26.76553778412699 27.318529525443218 24.835188248003618 22 21.819133430502184 30.18788922750762 28.34888034980787 19.644096992182327 23 20.364033291688294 29.346190261320455 29.70938891337745 20.580387533613806 24 22.19104031284145 27.721691874173327 30.077779055724886 20.00948875726034 25 21.38148242336509 28.337669620558565 29.764536343303533 20.51631161277281 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 829.0 1 829.0 2 1795.5 3 2762.0 4 2762.0 5 2762.0 6 3951.5 7 5141.0 8 5141.0 9 5141.0 10 6021.5 11 6902.0 12 6902.0 13 6902.0 14 8857.5 15 10813.0 16 10813.0 17 10813.0 18 15637.5 19 20462.0 20 20462.0 21 20462.0 22 28777.0 23 37092.0 24 37092.0 25 37092.0 26 48168.5 27 59245.0 28 59245.0 29 59245.0 30 71479.5 31 83714.0 32 83714.0 33 83714.0 34 89409.5 35 95105.0 36 95105.0 37 95105.0 38 96145.0 39 97185.0 40 97185.0 41 97185.0 42 95841.0 43 94497.0 44 94497.0 45 94497.0 46 90425.0 47 86353.0 48 86353.0 49 86353.0 50 76808.5 51 67264.0 52 67264.0 53 67264.0 54 55712.0 55 44160.0 56 44160.0 57 44160.0 58 35937.5 59 27715.0 60 27715.0 61 27715.0 62 21686.5 63 15658.0 64 15658.0 65 15658.0 66 11665.0 67 7672.0 68 7672.0 69 7672.0 70 5463.0 71 3254.0 72 3254.0 73 3254.0 74 2256.0 75 1258.0 76 1258.0 77 1258.0 78 834.5 79 411.0 80 411.0 81 411.0 82 270.0 83 129.0 84 129.0 85 129.0 86 80.5 87 32.0 88 32.0 89 32.0 90 20.5 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.868846095226681 2 37.234437507083186 3 5.977362462434688 4 14.205712662978417 5 14.236325054092747 6 15.487394859463071 7 9.565004435540073 8 7.872855667135188 9 0.10981381152927781 10 1.5286655733049528 11 5.985829719551418 12 6.003285295761291 13 16.887358124593735 14 1.6761261126301514 15 3.127804778919917 16 3.139268142401028 17 3.1005792906522784 18 1.591844337945166 19 1.5359604409747505 20 0.2826761222046653 21 4.619735482887673 22 1.6886316000640906 23 0.11267965239955553 24 9.392532921346998 25 0.11437310382290146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 767663.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.77485072527458 #Duplication Level Percentage of deduplicated Percentage of total 1 96.46617352909816 86.60236328613222 2 2.479575235384212 4.452069932374105 3 0.44838872948665387 1.2076209376967963 4 0.18830174945278852 0.6761904579372857 5 0.09843979940456463 0.4418709148485381 6 0.06502761116896986 0.35027064514292816 7 0.03919325700730609 0.24629981580877583 8 0.031214422136150402 0.22418160701988873 9 0.026885993633060525 0.21723174585078228 >10 0.13473267087816307 2.3010285799665837 >50 0.011336243701075763 0.707093370260365 >100 0.009823933984376348 1.7699901575800276 >500 4.5341233225723663E-4 0.33622350191893813 >1k 4.5341233225723663E-4 0.46756504746273636 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1285 0.16739115992303913 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1159 0.15097770766599405 No Hit CTGTAGGACGTGGAATATGGCAAGA 1002 0.13052602509173947 No Hit CTTTAGGACGTGAAATATGGCGAGG 938 0.12218903346911339 No Hit GTCCTACAGTGTGCATTTCTCATTT 906 0.11802053765780038 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.302654941035324E-4 0.0 3 0.0 0.0 0.0 1.302654941035324E-4 0.0 4 0.0 0.0 0.0 1.302654941035324E-4 0.0 5 0.0 1.302654941035324E-4 0.0 1.302654941035324E-4 0.0 6 0.0 1.302654941035324E-4 0.0 1.302654941035324E-4 0.0 7 0.0 1.302654941035324E-4 0.0 1.302654941035324E-4 0.0 8 0.0 1.302654941035324E-4 0.0 1.302654941035324E-4 0.0 9 0.0 1.302654941035324E-4 0.0 1.302654941035324E-4 0.0 10 0.0 1.302654941035324E-4 0.0 1.302654941035324E-4 0.0 11 0.0 1.302654941035324E-4 0.0 1.302654941035324E-4 0.0 12 0.0 1.302654941035324E-4 0.0 1.302654941035324E-4 0.0 13 0.0 1.302654941035324E-4 0.0 1.302654941035324E-4 1.302654941035324E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCTAC 85 1.5033766E-6 15.789737 1 CTAAGAC 70 1.5536316E-6 15.741535 3 CTATAGG 115 9.637006E-9 15.171877 1 TGTAAGA 110 6.7093424E-8 15.022779 2 TACTATA 65 6.5690366E-4 14.83018 2 GTCCTAA 445 0.0 14.476883 1 ATGTAAG 65 7.9829106E-4 14.453682 1 GTGCTAT 65 7.9829106E-4 14.453682 1 TATAGGA 125 2.0603693E-8 14.321716 2 GTAAGAC 70 2.7061942E-5 14.167381 3 TAGGACC 305 0.0 14.1323595 4 ATACAGC 45 0.006005463 13.973325 6 GTCCTAC 915 0.0 13.4946165 1 GTCTAGA 60 0.007838253 13.421276 1 TAGGCAG 55 0.0014593857 13.345457 5 TGTCGAG 70 1.6159047E-6 13.309677 18 TGTAGGA 745 0.0 13.30877 2 TAAGACC 65 2.769564E-4 13.262675 4 TCCTACA 1000 0.0 13.220045 2 TTAGGAC 485 0.0 13.177449 3 >>END_MODULE