FastQCFastQC Report
Thu 26 May 2016
SRR1926705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926705_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767663
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGGACGTGAAATATGGCGAGG27170.35393134747929755No Hit
CTGTAGGACGTGGAATATGGCAAGA27090.3528892235264693No Hit
GTCCTACAGTGGACATTTCTAAATT26820.34937205518567394No Hit
GTCCTAAAGTGTGTATTTCTCATTT25970.33829948818687366No Hit
GTCCTACAGTGTGCATTTCTCATTT20300.2644389530301708No Hit
CTGTAGGACCTGGAATATGGCGAGA17070.22236319843472985No Hit
CTGAAGGACCTGGAATATGGCGAGA14190.1848467361329125No Hit
GTATCAACGCAGAGTACTTTTTTTT14020.18263222273315244No Hit
CCCATGTACTCTGCGTTGATACCAC12530.16322266411172612No Hit
GTACATGGGAAGCAGTGGTATCAAC12050.15696992039475655No Hit
GTCCTTCAGTGTGCATTTCTCATTT8810.11476390030521207No Hit
GAATATGGCAAGAAAACTGAAAATC8580.11176779394083082No Hit
GGTATCAACGCAGAGTACTTTTTTT7920.10317027132999766No Hit
TATCAACGCAGAGTACTTTTTTTTT7680.10004389947151288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTC250.00610700118.95794913
TAGGACC6100.014.2316924
GTCGAGG400.00538416614.20642319
GGCGAGG6950.013.08217319
AGGACCT11500.012.2774695
TAAGACC701.0476615E-412.2656174
GGACCTG10900.012.2531166
CCTATAG1458.913048E-1111.8442193
TTAGTAC657.7295594E-411.7429873
GTCCTAA7800.011.7406761
CTAGGAC1552.3646862E-1111.6956373
TGTAGGA16450.011.54676152
TGGCGAG17450.011.45197818
GAATAAG751.9932546E-411.4471591
GTCCAAG751.9932546E-411.4471591
CTGTAGG16250.011.3297531
GTCCTAT1354.4128683E-911.3058361
TCTAATC855.0853094E-511.2249153
CTAATAC855.0853094E-511.2249153
GGCAAGA11250.011.19676619
Produced by FastQC (version 0.11.5)