FastQCFastQC Report
Thu 26 May 2016
SRR1926704_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926704_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750122
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGG300.00955469117.5408136
GGACCAT300.00955469117.5408136
GTCGACA350.002658581415.7956769
CTCCTAT751.3638877E-414.4162861
CCTATAC559.589614E-414.1084123
GTCCTAA2500.014.055881
CCTAATA801.8656788E-413.8941072
GAATAAG600.0074840813.5152681
TCCTAAA3200.013.4599162
GTCCTAC5400.013.2649861
TGTAGGA4600.012.6859252
CTGTAGG4700.012.6525921
ATAGACA600.00221179812.6277174
GTAGGAC4750.012.6020993
TCTGTAC808.960474E-512.4708283
CTATACT902.5100426E-512.0263984
GACGTGG2050.012.0218547
TTTAGAA1401.1326047E-611.9092352
CAGGACT1005.6204863E-611.9061344
TAGGACC2000.011.9061344
Produced by FastQC (version 0.11.5)