##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926704_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 750122 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.188412018311688 27.0 2.0 31.0 2.0 31.0 2 16.190948938972593 16.0 2.0 30.0 2.0 31.0 3 19.899249722045212 23.0 15.0 31.0 2.0 31.0 4 21.867036295429276 28.0 2.0 35.0 2.0 37.0 5 22.672886543788877 30.0 2.0 35.0 2.0 37.0 6 23.12605149562338 30.0 2.0 35.0 2.0 37.0 7 23.100201033965142 30.0 2.0 35.0 2.0 37.0 8 23.281931472480476 30.0 7.0 35.0 2.0 37.0 9 26.114446716667423 31.0 17.0 37.0 2.0 39.0 10 26.880402121254942 31.0 23.0 37.0 2.0 39.0 11 23.874356438019415 30.0 7.0 37.0 2.0 39.0 12 25.081616057121376 31.0 10.0 37.0 2.0 39.0 13 21.60158614198757 27.0 2.0 35.0 2.0 39.0 14 25.080914837853044 31.0 17.0 38.0 2.0 40.0 15 26.2093339483444 31.0 17.0 38.0 2.0 40.0 16 26.58239859649497 31.0 17.0 39.0 2.0 40.0 17 25.924656255915703 31.0 11.0 38.0 2.0 40.0 18 26.0549004028678 31.0 16.0 38.0 2.0 40.0 19 26.452699694183078 31.0 18.0 39.0 2.0 40.0 20 26.701849832427257 31.0 18.0 39.0 2.0 40.0 21 24.552067530348396 30.0 2.0 38.0 2.0 40.0 22 25.49443557181365 31.0 2.0 38.0 2.0 40.0 23 25.49534075790338 31.0 2.0 38.0 2.0 40.0 24 24.45830944832974 31.0 2.0 39.0 2.0 40.0 25 24.286884000202633 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.092020659484689 1101 2 10.895061873297493 1101 3 7.376218872144957 1101 4 8.902192560279559 1101 5 8.69140108338372 1101 6 7.913055853150514 1101 7 7.688799070295907 1101 8 6.156443299766362 1101 9 6.000553708600751 1101 10 5.704541507794293 1101 11 8.448984198447572 1101 12 5.954721872821658 1101 13 10.572056022374852 1101 14 7.867793018547484 1101 15 7.062129600692664 1101 16 6.523062505865841 1101 17 2.964852059590285 1101 18 5.094312601019681 1101 19 5.805526462456758 1101 20 3.9466212886367735 1101 21 1.1202959179732268 1101 22 4.771017469503548 1101 23 6.010335388700575 1101 24 7.25205978658542 1101 25 7.912927460476251 1102 1 5.690481991131712 1102 2 10.6791393924434 1102 3 6.884938953022097 1102 4 8.00956601485544 1102 5 8.141110756953385 1102 6 6.759283667149557 1102 7 6.808546205782946 1102 8 6.851786417184989 1102 9 5.99760654897128 1102 10 5.287326721105 1102 11 8.069926795706582 1102 12 5.780615177001181 1102 13 9.889528282586344 1102 14 7.223693579173105 1102 15 5.4804863517252365 1102 16 5.936532472546542 1102 17 6.835005569776058 1102 18 6.595772806477239 1102 19 5.520209586212772 1102 20 5.714120091803274 1102 21 4.985910377093649 1102 22 5.841522279471079 1102 23 6.4147917245107955 1102 24 7.871660832940254 1102 25 7.582017057695481 1103 1 5.0173404185642205 1103 2 9.203214358764246 1103 3 6.350440850929552 1103 4 7.893914454558491 1103 5 7.352205701462964 1103 6 6.583817652727916 1103 7 6.6449037248678025 1103 8 6.421746930336582 1103 9 5.403366332987911 1103 10 4.58727731136036 1103 11 5.52685978817637 1103 12 4.105305444782886 1103 13 8.461194250874026 1103 14 6.589759730904699 1103 15 5.514893004842897 1103 16 5.472059716834053 1103 17 2.9338277888741544 1103 18 1.6791469733702549 1103 19 3.906077853281996 1103 20 4.301312594625227 1103 21 6.6486329086196605 1103 22 2.081995548389756 1103 23 4.532039547138002 1103 24 5.711378760624591 1103 25 2.0858067305128856 1104 1 6.24989873069028 1104 2 10.81015163291692 1104 3 7.470670564138871 1104 4 8.179096036481528 1104 5 8.330375255287155 1104 6 7.843628096151054 1104 7 7.949452515985847 1104 8 7.7215871673902505 1104 9 6.792798634788753 1104 10 5.67651179856718 1104 11 8.659473828424762 1104 12 5.545434145710431 1104 13 9.906627918216266 1104 14 7.829814352560671 1104 15 6.976367735580261 1104 16 6.598777775714257 1104 17 6.741718243307979 1104 18 4.200605673839018 1104 19 5.5369148006222915 1104 20 4.824476197819404 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2208 21 6.880468990206072 2208 22 6.030926274498654 2208 23 1.602383408095175 2208 24 3.179118987920564 2208 25 4.494101901789765 2209 1 2.8385773284163918 2209 2 4.668978245669177 2209 3 5.05031038592179 2209 4 -0.09718599080016332 2209 5 4.408320891589526 2209 6 4.8100401669886 2209 7 5.717905721522058 2209 8 4.3006366762948645 2209 9 3.9441377589291804 2209 10 2.985008934364103 2209 11 -0.7973035375068243 2209 12 2.750182233121663 2209 13 1.5400947895580117 2209 14 3.0451924398471064 2209 15 3.4966742489902245 2209 16 3.0921202195846114 2209 17 0.9433431637467855 2209 18 3.0990493944325763 2209 19 3.6154900623942297 2209 20 3.7779993548076867 2209 21 5.592209808377458 2209 22 4.225992729583748 2209 23 0.05484108478398042 2209 24 -3.4509552615385886 2209 25 -0.33443026504622253 2210 1 -1.7189843054139757 2210 2 -14.114929800307834 2210 3 -7.739185092510237 2210 4 -2.007835970064704 2210 5 -0.3511576889909449 2210 6 -1.1622603548022035 2210 7 -0.8520359097086398 2210 8 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-6.5432717743336255 2211 21 -4.395520640123781 2211 22 -5.884676641613005 2211 23 -7.539001601715906 2211 24 -22.348273269201425 2211 25 -22.14285938382017 2212 1 -1.7937052853580226 2212 2 -14.114929800307834 2212 3 -8.270587777801225 2212 4 -19.763086373941924 2212 5 -20.54340324634151 2212 6 -20.985362131861976 2212 7 -10.435942633771841 2212 8 -9.872445862720182 2212 9 -5.8206318887672985 2212 10 -6.04542104881504 2212 11 -6.118528773466078 2212 12 -3.3974459091080327 2212 13 -6.80331587299689 2212 14 -4.492606597626029 2212 15 -4.528205435151445 2212 16 -4.578970099077196 2212 17 -4.818117797845591 2212 18 -3.7509167302286706 2212 19 -4.24686509132972 2212 20 -4.4294613965239655 2212 21 -2.3736058864174225 2212 22 -3.8273171246354813 2212 23 -3.9100967714911334 2212 24 -2.8017793667624 2212 25 -3.278530115527488 2213 1 -19.09782113901656 2213 2 -14.114929800307834 2213 3 -9.117909277831238 2213 4 -6.262676201669569 2213 5 -20.54340324634151 2213 6 -11.065755897243436 2213 7 -20.974814584991353 2213 8 -11.048918106062386 2213 9 -8.972715763994891 2213 10 -7.5420652643855774 2213 11 -6.862756974310436 2213 12 -5.578286237045766 2213 13 -19.39630367787494 2213 14 -12.809613490521043 2213 15 -7.504354167619084 2213 16 -6.858956443341786 2213 17 -5.450520281888888 2213 18 -5.474444361833946 2213 19 -6.29775771623218 2213 20 -6.2696730354123 2213 21 -4.520304374782533 2213 22 -5.421721149325855 2213 23 -5.220106975776936 2213 24 -4.256394680194042 2213 25 -4.919725667659382 2214 1 -19.09782113901656 2214 2 -14.114929800307834 2214 3 -12.52635868372457 2214 4 -19.763086373941924 2214 5 -20.54340324634151 2214 6 -20.985362131861976 2214 7 -16.73962294736069 2214 8 -15.133007709410078 2214 9 -14.107217254620956 2214 10 -14.0346414320903 2214 11 -14.781094104476534 2214 12 -12.585925874264829 2214 13 -19.39630367787494 2214 14 -16.94796374048317 2214 15 -24.02363226441974 2214 16 -18.47596487259636 2214 17 -23.776197066138273 2214 18 -18.010280841726928 2214 19 -15.212239655789652 2214 20 -13.843877772830586 2214 21 -11.479768778403484 2214 22 -12.449594999199944 2214 23 -12.417065412606117 2214 24 -11.293395220420937 2214 25 -11.026134645144213 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 136605.0 3 6.0 4 621.0 5 2335.0 6 3297.0 7 3273.0 8 2811.0 9 2566.0 10 2425.0 11 2206.0 12 2194.0 13 2249.0 14 3506.0 15 7826.0 16 12132.0 17 12569.0 18 13059.0 19 14972.0 20 17610.0 21 20229.0 22 20661.0 23 22099.0 24 23211.0 25 23591.0 26 19489.0 27 15129.0 28 15896.0 29 18616.0 30 18145.0 31 20693.0 32 27025.0 33 34487.0 34 42401.0 35 48738.0 36 48028.0 37 50215.0 38 39207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.455109044269136 19.16947446179165 12.895026384442998 25.48039010949622 2 13.454221855372273 21.02351135586881 42.031116327258815 23.4911504615001 3 18.126416749251046 25.83082732460787 30.173626536354213 25.869129389786877 4 11.519808892868548 17.67365263836795 39.58146786807848 31.22507060068503 5 12.059934694566582 38.07628762526123 37.09936335799351 12.764414322178682 6 29.58520652147967 38.23117992950182 19.711005195859226 12.47260835315929 7 26.22007672899048 31.66638566226821 23.7152922593641 18.398245349377206 8 23.756606842074692 37.58450949642795 21.999767671487483 16.659115990009873 9 26.338131733362513 15.502430783068458 20.776355947244735 37.38308153632429 10 14.227746437437041 29.750637539808107 35.32695861312553 20.694657409629325 11 32.288428484562814 22.624257190989315 26.014300096976363 19.073014227471507 12 23.059822409569534 25.294167104871267 32.586034169983485 19.059976315575717 13 27.864383071520738 22.186695073994507 26.08660457420578 23.86231728027898 14 20.042716300491385 22.995382131660165 28.68889742792473 28.273004139923717 15 22.247602715176644 31.611626073195076 24.241939663966562 21.898831547661718 16 20.937609908487175 28.609377478328636 28.93322391332832 21.51978869985587 17 20.661022625319905 28.554319648623284 29.458808742521736 21.325848983535074 18 20.338934764825346 28.02357240823569 31.376562981171645 20.26092984576732 19 22.278521861042787 27.27200002169427 28.2097347604546 22.23974335680834 20 22.719143475820033 27.978854225145028 28.80744245729409 20.494559841740852 21 21.959633398193365 26.66210261539778 27.39005944022098 23.988204546187873 22 21.933602926598752 28.572223927171798 28.4983528376547 20.99582030857475 23 21.59219356999777 28.137231744049025 28.814102179143795 21.45647250680941 24 22.018580519278952 27.63279454261916 29.003939306593352 21.34468563150854 25 21.99766980340134 27.63044630071281 28.924999966635216 21.44688392925064 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 826.0 1 826.0 2 1793.5 3 2761.0 4 2761.0 5 2761.0 6 3986.0 7 5211.0 8 5211.0 9 5211.0 10 6182.0 11 7153.0 12 7153.0 13 7153.0 14 8898.5 15 10644.0 16 10644.0 17 10644.0 18 15083.0 19 19522.0 20 19522.0 21 19522.0 22 27129.5 23 34737.0 24 34737.0 25 34737.0 26 44534.0 27 54331.0 28 54331.0 29 54331.0 30 64452.0 31 74573.0 32 74573.0 33 74573.0 34 80851.0 35 87129.0 36 87129.0 37 87129.0 38 89617.0 39 92105.0 40 92105.0 41 92105.0 42 92032.0 43 91959.0 44 91959.0 45 91959.0 46 88682.0 47 85405.0 48 85405.0 49 85405.0 50 77504.5 51 69604.0 52 69604.0 53 69604.0 54 59310.5 55 49017.0 56 49017.0 57 49017.0 58 40337.0 59 31657.0 60 31657.0 61 31657.0 62 24912.5 63 18168.0 64 18168.0 65 18168.0 66 13635.5 67 9103.0 68 9103.0 69 9103.0 70 6529.0 71 3955.0 72 3955.0 73 3955.0 74 2732.5 75 1510.0 76 1510.0 77 1510.0 78 1016.5 79 523.0 80 523.0 81 523.0 82 339.5 83 156.0 84 156.0 85 156.0 86 107.5 87 59.0 88 59.0 89 59.0 90 36.0 91 13.0 92 13.0 93 13.0 94 7.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.458448092443628 2 37.98048850720283 3 6.373629889537968 4 14.839986028939292 5 14.712140158534211 6 16.228960089158832 7 9.861862470371486 8 8.190934274691317 9 0.10331652717824567 10 1.6691951442565343 11 6.384161509727751 12 6.451617203601548 13 17.655927969050367 14 1.8177043200972642 15 3.295197314570163 16 3.3443893126718054 17 3.2686682966237495 18 1.7315849955073976 19 1.6797267644463167 20 0.2642236862803651 21 4.973191027592845 22 1.8269028238073273 23 0.10544951354579656 24 9.812670472269843 25 0.11051535616872989 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 750122.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.49705925480772 #Duplication Level Percentage of deduplicated Percentage of total 1 96.54344849719381 89.29985076308414 2 2.5306734451378525 4.6815970321896865 3 0.44572047911261664 1.2368350070268297 4 0.17482871665291633 0.6468456865474717 5 0.08453216758210885 0.39094884568898297 6 0.04958257560064847 0.2751745460003499 7 0.0320078039439842 0.2072439416616082 8 0.024135471063700658 0.17859680776974537 9 0.016446756493345524 0.13691489489229372 >10 0.0867774535127814 1.4589109279483832 >50 0.006663895623709726 0.4683135086003615 >100 0.00488658162782483 0.8671444563106971 >500 2.9615645465053163E-4 0.15162358227947964 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.333116479719299E-4 0.0 6 0.0 0.0 0.0 1.333116479719299E-4 0.0 7 0.0 0.0 0.0 1.333116479719299E-4 0.0 8 0.0 0.0 0.0 2.666232959438598E-4 0.0 9 0.0 0.0 0.0 2.666232959438598E-4 0.0 10 0.0 0.0 0.0 2.666232959438598E-4 0.0 11 0.0 0.0 0.0 2.666232959438598E-4 0.0 12 0.0 0.0 0.0 2.666232959438598E-4 0.0 13 0.0 0.0 0.0 2.666232959438598E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCGG 30 0.009554691 17.540813 6 GGACCAT 30 0.009554691 17.540813 6 GTCGACA 35 0.0026585814 15.795676 9 CTCCTAT 75 1.3638877E-4 14.416286 1 CCTATAC 55 9.589614E-4 14.108412 3 GTCCTAA 250 0.0 14.05588 1 CCTAATA 80 1.8656788E-4 13.894107 2 GAATAAG 60 0.00748408 13.515268 1 TCCTAAA 320 0.0 13.459916 2 GTCCTAC 540 0.0 13.264986 1 TGTAGGA 460 0.0 12.685925 2 CTGTAGG 470 0.0 12.652592 1 ATAGACA 60 0.002211798 12.627717 4 GTAGGAC 475 0.0 12.602099 3 TCTGTAC 80 8.960474E-5 12.470828 3 CTATACT 90 2.5100426E-5 12.026398 4 GACGTGG 205 0.0 12.021854 7 TTTAGAA 140 1.1326047E-6 11.909235 2 CAGGACT 100 5.6204863E-6 11.906134 4 TAGGACC 200 0.0 11.906134 4 >>END_MODULE