FastQCFastQC Report
Thu 26 May 2016
SRR1926704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926704_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750122
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14300.19063565659985976No Hit
CTGTAGGACGTGGAATATGGCAAGA12370.16490650854127728No Hit
GTCCTACAGTGGACATTTCTAAATT12020.16024060086225975No Hit
CTTTAGGACGTGAAATATGGCGAGG11560.15410826505555097No Hit
GTCCTAAAGTGTGTATTTCTCATTT11030.14704274771303866No Hit
GTACATGGGAAGCAGTGGTATCAAC9620.12824580534899657No Hit
CCCATGTACTCTGCGTTGATACCAC9500.1266460655733334No Hit
GTCCTACAGTGTGCATTTCTCATTT9310.12411314426186673No Hit
GGTATCAACGCAGAGTACTTTTTTT9110.12144691130242814No Hit
TATCAACGCAGAGTACTTTTTTTTT7950.10598276013768428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2900.013.1556124
GGCGAGG3250.013.11642119
GTCTAAG1102.581146E-913.0052441
TAAGTCT550.002975976612.1390424
TACTATT550.003056204212.0960167
CAGGACT1459.094947E-1111.8400514
GTCCTAG1001.8365918E-611.4446141
TGTAGGA8850.011.3221352
GTAGGAC8200.011.2848153
GTGTTGG1104.7103458E-711.2712121
AGGACCT5600.011.2410015
GGACGTG8150.011.2339786
TATACCT855.103228E-511.22096355
AATCCCG1105.1403185E-711.19532919
ACTGATC855.294865E-511.1804448
TGGCGAG7800.011.17324618
GTCCTAC7550.011.1161811
TTAGGAC5400.010.9530343
GCCTTAC700.001442056710.8996321
CCCTTAT1053.2996159E-610.8996321
Produced by FastQC (version 0.11.5)