FastQCFastQC Report
Thu 26 May 2016
SRR1926703_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926703_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767929
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13270.1728024335583107No Hit
GTCCTAAAGTGTGTATTTCTCATTT10800.14063800169026044No Hit
CTGTAGGACGTGGAATATGGCAAGA9110.11863075883317338No Hit
CTTTAGGACGTGAAATATGGCGAGG8870.11550546990672314No Hit
GTCCTACAGTGTGCATTTCTCATTT7940.1033949753167285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACA258.19145E-426.7862451
CCTACCG300.00748905518.2982123
CTCCTAG450.00116942317.8574961
GTCTAAG552.2767698E-417.0457921
GTATTGG500.002366846416.0717471
GTCCTAC8300.015.9749291
GTCCTAA4250.015.7566141
ACAGTCG400.002782153415.6880826
GGACGTT507.241152E-414.642216
GTCCTAG550.004465012314.6106781
GATATAG550.004465012314.6106781
GTCTTAC550.004465012314.6106781
GTTCTGG751.4735761E-414.2859971
TAGGACC3450.014.0078734
AGGACCG450.006075990413.9487465
CTTAGGA600.006716026513.7383312
TCCTACA9500.013.738332
TCCTAAA5050.013.0582142
TTTAGAA2450.012.8972072
TTAAACT1004.3657383E-712.8872424
Produced by FastQC (version 0.11.5)