##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926703_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 767929 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.606545657215705 27.0 10.0 31.0 2.0 31.0 2 16.587486603579237 20.0 2.0 31.0 2.0 31.0 3 20.28143357003056 25.0 15.0 31.0 2.0 31.0 4 22.346160908104785 28.0 7.0 35.0 2.0 37.0 5 23.17490028375019 30.0 7.0 35.0 2.0 37.0 6 23.598039662520883 30.0 2.0 35.0 2.0 37.0 7 23.564946759400936 31.0 7.0 35.0 2.0 37.0 8 23.733208408589856 31.0 11.0 35.0 2.0 37.0 9 26.622054903513217 32.0 17.0 37.0 2.0 39.0 10 27.394377605221315 32.0 25.0 38.0 2.0 39.0 11 24.425570593114728 30.0 8.0 37.0 2.0 39.0 12 25.607294424354336 31.0 17.0 37.0 2.0 39.0 13 22.166964654284445 28.0 2.0 37.0 2.0 39.0 14 25.619962262136212 31.0 17.0 38.0 2.0 40.0 15 26.7607903855695 31.0 17.0 38.0 2.0 40.0 16 27.128653820861043 32.0 18.0 39.0 2.0 40.0 17 26.46868265165139 31.0 17.0 38.0 2.0 40.0 18 26.569906853367954 31.0 17.0 38.0 2.0 40.0 19 26.9632166515394 31.0 18.0 39.0 2.0 40.0 20 27.23672631193769 31.0 22.0 39.0 2.0 40.0 21 25.053795337850243 30.0 8.0 38.0 2.0 40.0 22 26.003228163020278 31.0 11.0 38.0 2.0 40.0 23 26.0080202727075 31.0 2.0 39.0 2.0 40.0 24 24.991613808047358 31.0 2.0 39.0 2.0 40.0 25 24.808835191795076 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.8375799969437345 1101 2 10.719016952956164 1101 3 7.414356067255831 1101 4 8.905434182939171 1101 5 8.621873111037516 1101 6 7.946607017315717 1101 7 7.601537690651085 1101 8 6.318297261878726 1101 9 6.0728025639563725 1101 10 5.722452888209212 1101 11 8.660601036056981 1101 12 6.119843980904239 1101 13 10.675122319215962 1101 14 7.811527798949527 1101 15 6.999848218689433 1101 16 6.577483278556649 1101 17 2.6550340930094194 1101 18 5.256662993923463 1101 19 5.670031735050305 1101 20 3.628277053622199 1101 21 1.1420822540049258 1101 22 4.8977507993140215 1101 23 5.9246178090493835 1101 24 7.386975436072849 1101 25 7.905125588670593 1102 1 5.962678374543035 1102 2 10.949771686933651 1102 3 7.0148126341383765 1102 4 8.084484969485857 1102 5 8.313800695642254 1102 6 6.665261306579048 1102 7 6.548055834799161 1102 8 6.9241562998169215 1102 9 5.988519037344645 1102 10 5.307009027445517 1102 11 8.107803082311051 1102 12 5.536511042661665 1102 13 10.143252718131201 1102 14 7.538499143806867 1102 15 5.484651842777961 1102 16 5.939352832230519 1102 17 6.708630920272583 1102 18 6.651568456606348 1102 19 5.279850767678479 1102 20 5.714182032713996 1102 21 4.767813567002147 1102 22 5.5609710892790325 1102 23 5.941963083605856 1102 24 7.5731840819229745 1102 25 7.407774672218064 1103 1 5.132608556283696 1103 2 9.626404184698163 1103 3 6.385774396945791 1103 4 8.045074664334464 1103 5 7.413263586556404 1103 6 6.733584898518263 1103 7 6.902166466242839 1103 8 6.2557699365843185 1103 9 5.438861022457186 1103 10 4.5992792756290015 1103 11 5.51985931591501 1103 12 4.488183660757564 1103 13 8.534680883492108 1103 14 6.868255697781294 1103 15 5.814918265465639 1103 16 5.670720121280482 1103 17 3.3456582850034025 1103 18 2.3995742836773566 1103 19 4.0920159627728445 1103 20 4.466962031174322 1103 21 6.709231961359837 1103 22 2.6820261207535054 1103 23 4.819986024737041 1103 24 6.416864019388555 1103 25 2.709460614409256 1104 1 5.757368732586521 1104 2 10.43754986499085 1104 3 7.066404132203477 1104 4 7.7039315767303975 1104 5 7.86539103160581 1104 6 7.00537356558824 1104 7 7.1453981085670435 1104 8 7.137430985758979 1104 9 6.103151057890599 1104 10 5.014723057497463 1104 11 7.869347980104219 1104 12 4.745983332350338 1104 13 9.274236669508873 1104 14 6.977525999973558 1104 15 6.448214148852262 1104 16 5.863593108345846 1104 17 6.179755021096511 1104 18 3.8160464986976663 1104 19 4.554185755069678 1104 20 3.945255465910588 1104 21 3.1446308032922516 1104 22 5.199601668819071 1104 23 5.55758289046458 1104 24 6.693832302164459 1104 25 5.537522147552458 1105 1 3.8085896205152707 1105 2 9.953127819629888 1105 3 6.7611877905240725 1105 4 5.900703514075637 1105 5 2.462962792967204 1105 6 4.985750238703886 1105 7 6.441219620335961 1105 8 3.267024474356692 1105 9 4.327478004288849 1105 10 4.023649161203842 1105 11 7.2949818321762585 1105 12 4.522556046957561 1105 13 5.4718168916320735 1105 14 4.196274484087066 1105 15 4.883063462684483 1105 16 5.019004893622995 1105 17 5.743310571638279 1105 18 3.1707498361861752 1105 19 3.2236649669779176 1105 20 2.369487087725819 1105 21 3.0661487767705857 1105 22 4.588598166063683 1105 23 2.146080766813366 1105 24 5.082499393749387 1105 25 5.896167635992239 1106 1 6.604707932604505 1106 2 11.356364839647224 1106 3 8.050253891129941 1106 4 9.235664496473703 1106 5 9.03367834180957 1106 6 7.770380252709629 1106 7 7.682926908144463 1106 8 7.463784987354899 1106 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2.1613446175334836 1107 23 -0.8949891118081617 1107 24 2.5610557533088283 1107 25 3.422202115998889 1108 1 5.608321456862967 1108 2 11.012202249376472 1108 3 0.3784342073914466 1108 4 6.236478697958123 1108 5 7.614273511778844 1108 6 7.354328143814627 1108 7 7.769241964782925 1108 8 7.2933172768291215 1108 9 5.882912104342346 1108 10 4.788386668579967 1108 11 7.1822741310981435 1108 12 2.8341283524347958 1108 13 3.7537711220786534 1108 14 5.318003907070064 1108 15 5.28341859833213 1108 16 5.236422441953842 1108 17 0.4488043567644482 1108 18 3.680193166737631 1108 19 4.998958646201615 1108 20 5.201574345300845 1108 21 5.236401325488153 1108 22 1.8781267486419146 1108 23 4.818495445825587 1108 24 5.905635783470466 1108 25 6.414533168254902 1109 1 4.0742926719506904 1109 2 -14.140645623730954 1109 3 -5.711011158521414 1109 4 2.49367977505095 1109 5 2.208887936820421 1109 6 3.12568587595678 1109 7 4.424011436660528 1109 8 4.45880031993223 1109 9 3.8490371027490937 1109 10 3.092341199701554 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2204 7 4.841875934147033 2204 8 4.774377630445564 2204 9 3.2680137493786745 2204 10 2.830391908770032 2204 11 4.664161216403524 2204 12 4.6151964721517125 2204 13 3.059094536100485 2204 14 4.11416304713692 2204 15 4.389712279204886 2204 16 4.290524072118917 2204 17 -0.7274541783727528 2204 18 2.603871676615725 2204 19 3.363268622666478 2204 20 3.6446402916112035 2204 21 5.1143143704990415 2204 22 4.556571633838573 2204 23 3.153248525139503 2204 24 4.931683614938372 2204 25 5.045145483968366 2205 1 5.820307838105119 2205 2 10.199088497391646 2205 3 6.726797730675283 2205 4 7.270678947372662 2205 5 7.856163013258612 2205 6 6.949049224454754 2205 7 7.195354239662876 2205 8 6.853077191376421 2205 9 5.599622665269457 2205 10 4.9616861076635 2205 11 7.6802485698355625 2205 12 6.667232541044154 2205 13 9.77497189013059 2205 14 7.249116719756763 2205 15 5.892256307424471 2205 16 5.451710743645322 2205 17 6.144124586072465 2205 18 6.071258013452059 2205 19 4.509375057720693 2205 20 5.079676451905858 2205 21 -1.2962350244973102 2205 22 3.247060048890372 2205 23 4.504954643332717 2205 24 6.580646086995813 2205 25 6.885081592694117 2206 1 4.364262352445483 2206 2 9.0759101781907 2206 3 6.6104721737041245 2206 4 1.9137938154758203 2206 5 6.11297729215741 2206 6 6.5440206266999965 2206 7 6.673013710765442 2206 8 6.505060147354765 2206 9 5.162682925253016 2206 10 4.047407318037951 2206 11 3.9956126439812074 2206 12 5.019681991414849 2206 13 8.496609675193817 2206 14 0.5332317832788256 2206 15 2.8502947383526234 2206 16 3.734194223106144 2206 17 4.873041452252004 2206 18 -0.5478732785725597 2206 19 1.755605601804028 2206 20 3.1646487384489177 2206 21 4.180063064655194 2206 22 -1.33692368002977 2206 23 -2.5555591664515482 2206 24 1.7426483556018226 2206 25 3.3923415686106466 2207 1 1.5397898755339803 2207 2 -14.140645623730954 2207 3 -6.151240203861912 2207 4 -4.2488727313370696 2207 5 1.5343415610826554 2207 6 2.284573818472701 2207 7 3.4889066547071685 2207 8 3.4498674824060025 2207 9 0.9525143309380795 2207 10 0.5146722548150819 2207 11 -1.1289653708407101 2207 12 -2.158585522218516 2207 13 0.6002499007852613 2207 14 -5.495651312677442 2207 15 -1.5229299275549693 2207 16 -0.2529414713466238 2207 17 0.9045896301416434 2207 18 0.78892915579085 2207 19 0.2678573213728228 2207 20 0.01457516154607319 2207 21 -3.772594692880613 2207 22 -4.134215983419633 2207 23 -1.2549958536931136 2207 24 0.4306157021057544 2207 25 1.0901119554054262 2208 1 5.099313838381747 2208 2 6.744986970979554 2208 3 6.487685176128515 2208 4 8.625897857693708 2208 5 4.174863963399197 2208 6 6.633228048113349 2208 7 7.003576614159115 2208 8 6.989615951389638 2208 9 5.48888361659257 2208 10 4.887964954233588 2208 11 6.645265257214078 2208 12 6.339938198070751 2208 13 9.039506169205218 2208 14 3.4304279116505327 2208 15 5.180197789153876 2208 16 5.376652382693258 2208 17 6.285580342083037 2208 18 0.8330941213282266 2208 19 3.6775332589258163 2208 20 4.945625225190849 2208 21 7.4668749411565685 2208 22 6.703707179199743 2208 23 2.457337280012432 2208 24 3.9262442827950927 2208 25 5.48442138277418 2209 1 3.3491003104066337 2209 2 5.4530575246948345 2209 3 5.639264318651605 2209 4 0.7344633033482921 2209 5 5.137568100965865 2209 6 5.88002834346246 2209 7 6.456572596511041 2209 8 5.130932355374924 2209 9 4.554952976480173 2209 10 3.4846365969683326 2209 11 -0.3866540940466763 2209 12 3.2311621715021914 2209 13 2.620188129868918 2209 14 3.8879125360288356 2209 15 4.271206787871101 2209 16 3.81603079927482 2209 17 1.4330978985299723 2209 18 3.447050545546425 2209 19 4.2447562592912504 2209 20 4.312322684181154 2209 21 6.410381386647192 2209 22 5.418274663148974 2209 23 0.6042772124765072 2209 24 -2.926264416393554 2209 25 0.2669818537895665 2210 1 -2.3672347039043906 2210 2 -14.140645623730954 2210 3 -7.782059110542889 2210 4 -2.1497877529417444 2210 5 -0.5022710703575619 2210 6 -1.238480583892045 2210 7 -1.2989132969058872 2210 8 -21.279862247323727 2210 9 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-5.179064799350719 2211 22 -6.550985025188677 2211 23 -8.409254657951916 2211 24 -22.608423336933285 2211 25 -22.437665822372352 2212 1 -1.5351809286034914 2212 2 -14.140645623730954 2212 3 -8.225001465123174 2212 4 -19.863737596201933 2212 5 -20.613556336815243 2212 6 -21.04725801335913 2212 7 -10.229916334949156 2212 8 -9.983565951027431 2212 9 -5.748686870263121 2212 10 -6.127907825264998 2212 11 -6.280491897367238 2212 12 -3.446873810506805 2212 13 -6.249099448564088 2212 14 -4.354551879911341 2212 15 -4.438679009970716 2212 16 -4.658930794002615 2212 17 -4.761722515337166 2212 18 -3.9794433834150205 2212 19 -4.589625161109659 2212 20 -4.6401420556711415 2212 21 -2.3872718700577877 2212 22 -3.744868302405287 2212 23 -3.9177002247308153 2212 24 -3.0166023492789655 2212 25 -3.5001658223723524 2213 1 -19.15015006440596 2213 2 -14.140645623730954 2213 3 -8.5304479456351 2213 4 -5.321852779448005 2213 5 -20.613556336815243 2213 6 -10.533296407774488 2213 7 -21.07791016210965 2213 8 -10.601851776119538 2213 9 -8.127540855138047 2213 10 -6.884731075216518 2213 11 -6.27174165497825 2213 12 -4.599142571414308 2213 13 -19.60095130041594 2213 14 -12.44826754147168 2213 15 -6.873007754072585 2213 16 -6.056464890053292 2213 17 -4.632751375785748 2213 18 -4.741486076195063 2213 19 -5.585577265046059 2213 20 -5.526570696353712 2213 21 -3.6062581993189866 2213 22 -4.47548526317059 2213 23 -4.303979392205445 2213 24 -3.2270969844027455 2213 25 -4.240458143489281 2214 1 -19.15015006440596 2214 2 -14.140645623730954 2214 3 -12.04984428103742 2214 4 -19.863737596201933 2214 5 -20.613556336815243 2214 6 -21.04725801335913 2214 7 -16.427631868974213 2214 8 -14.622162803909998 2214 9 -13.361910947772913 2214 10 -13.096105963105991 2214 11 -14.306972691705175 2214 12 -11.684814441304578 2214 13 -19.60095130041594 2214 14 -16.550029902770316 2214 15 -24.33374073836578 2214 16 -18.15103436943952 2214 17 -24.08039535484334 2214 18 -17.64762892362437 2214 19 -14.393797840282339 2214 20 -13.051092546978778 2214 21 -10.554080260080465 2214 22 -11.55709201343852 2214 23 -11.446391330802875 2214 24 -10.331985489066867 2214 25 -10.127832798253614 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 130171.0 3 1.0 4 546.0 5 2341.0 6 3275.0 7 3177.0 8 2672.0 9 2559.0 10 2378.0 11 2186.0 12 2101.0 13 2286.0 14 3597.0 15 7944.0 16 11918.0 17 12501.0 18 12853.0 19 14638.0 20 17633.0 21 20298.0 22 20729.0 23 22734.0 24 23875.0 25 24821.0 26 20562.0 27 15870.0 28 16767.0 29 19553.0 30 18988.0 31 21813.0 32 28214.0 33 35652.0 34 44518.0 35 51480.0 36 51593.0 37 54002.0 38 41683.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.008577512340715 19.404362845637905 12.725468405058562 25.861591236962816 2 12.96216086890162 20.920217307906494 43.119201719322994 22.998420103868888 3 18.17580242128069 25.513431947338184 30.083270459406513 26.227495171974613 4 11.46737536546061 17.56871566484352 39.60020801398048 31.363700955715395 5 11.59260619414861 38.449331936906916 37.67177631787743 12.286285551067042 6 29.772915707047442 38.51512359895594 19.611546138492248 12.100414555504376 7 26.294693468840457 31.43922609010917 23.64070853499226 18.625371906058113 8 23.196534633881697 39.24349113596419 21.683969008369548 15.876005221784562 9 26.36840890810179 15.17357449121796 20.83102478629916 37.626991814381086 10 14.01597042337886 29.765970753947318 36.006177663357015 20.211881159316807 11 32.478019566773305 22.015596382348097 26.68634326715554 18.82004078372306 12 23.75032525591412 24.67211435721127 33.20752157761575 18.37003880925886 13 28.353152040133466 22.363550426558472 25.81096026743165 23.472337265876416 14 19.263453839725926 23.650312070018103 28.40320532260478 28.68302876765119 15 21.30755482386938 33.9367506240743 23.59813240983318 21.157562142223142 16 20.20627479652069 28.200968744288062 30.556571010784133 21.03618544840711 17 20.088031453995576 29.38744444340039 30.0618421635495 20.462681939054526 18 19.973723659159077 27.536043968547773 33.01808780743385 19.4721445648593 19 22.643031413024072 26.866433487830843 27.809683753958858 22.680851345186223 20 22.82603018067664 28.35249442404425 29.182097395101515 19.639378000177597 21 21.143787638063102 26.77537003368658 27.26128664166107 24.819555686589247 22 22.05908259456911 29.740773559989243 28.239005956472845 19.961137888968803 23 20.63870949238539 29.213326415305872 29.408873673863674 20.73909041844506 24 22.38955029781364 27.58508704492711 29.772023480216408 20.253339177042843 25 21.506085668693483 28.267592119398007 29.53700420312679 20.68931800878172 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 877.0 1 877.0 2 1789.0 3 2701.0 4 2701.0 5 2701.0 6 3856.5 7 5012.0 8 5012.0 9 5012.0 10 6020.0 11 7028.0 12 7028.0 13 7028.0 14 8699.0 15 10370.0 16 10370.0 17 10370.0 18 15135.0 19 19900.0 20 19900.0 21 19900.0 22 27813.5 23 35727.0 24 35727.0 25 35727.0 26 46652.5 27 57578.0 28 57578.0 29 57578.0 30 69246.5 31 80915.0 32 80915.0 33 80915.0 34 86880.5 35 92846.0 36 92846.0 37 92846.0 38 94648.5 39 96451.0 40 96451.0 41 96451.0 42 95037.5 43 93624.0 44 93624.0 45 93624.0 46 90419.0 47 87214.0 48 87214.0 49 87214.0 50 78214.5 51 69215.0 52 69215.0 53 69215.0 54 58085.5 55 46956.0 56 46956.0 57 46956.0 58 38527.5 59 30099.0 60 30099.0 61 30099.0 62 23562.5 63 17026.0 64 17026.0 65 17026.0 66 12841.0 67 8656.0 68 8656.0 69 8656.0 70 6143.0 71 3630.0 72 3630.0 73 3630.0 74 2509.0 75 1388.0 76 1388.0 77 1388.0 78 943.5 79 499.0 80 499.0 81 499.0 82 334.0 83 169.0 84 169.0 85 169.0 86 105.5 87 42.0 88 42.0 89 42.0 90 23.5 91 5.0 92 5.0 93 5.0 94 3.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.525599371816927 2 36.86369443008403 3 5.935965434304474 4 13.8600052869471 5 13.834612314419692 6 15.187471758456836 7 9.487986519587096 8 7.829499862617507 9 0.11094775688898324 10 1.517848655279329 11 5.935704993560602 12 5.914739513678999 13 16.520798146703665 14 1.6681229644928113 15 3.024498358572212 16 3.1087509392144326 17 2.990901502612872 18 1.5777500263696251 19 1.5254014368515838 20 0.2788018163137478 21 4.595867586717001 22 1.6866142573076417 23 0.11081753651704779 24 9.378992068277144 25 0.11472414767511058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 767929.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.27648466057076 #Duplication Level Percentage of deduplicated Percentage of total 1 96.45207256430668 87.07354049331889 2 2.498843632937629 4.511736377961175 3 0.45254367229710796 1.2256215567110467 4 0.17616381840493336 0.6361380099992215 5 0.1154537097828607 0.5211377530109206 6 0.06456024614555318 0.3496963242505022 7 0.04244535087944364 0.2682271947306446 8 0.02938630670699963 0.21223139733322235 9 0.025358228066241786 0.20603265183371983 >10 0.12191243927217865 2.0472206501890278 >50 0.011528901873403266 0.6983964928984787 >100 0.008832871249275807 1.4999239682087817 >500 5.98838718472091E-4 0.42478473480893464 >1k 2.994193592360455E-4 0.3253123947454997 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1327 0.1728024335583107 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1080 0.14063800169026044 No Hit CTGTAGGACGTGGAATATGGCAAGA 911 0.11863075883317338 No Hit CTTTAGGACGTGAAATATGGCGAGG 887 0.11550546990672314 No Hit GTCCTACAGTGTGCATTTCTCATTT 794 0.1033949753167285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.3022037193542632E-4 0.0 6 0.0 0.0 0.0 1.3022037193542632E-4 0.0 7 0.0 0.0 0.0 1.3022037193542632E-4 0.0 8 0.0 0.0 0.0 1.3022037193542632E-4 0.0 9 0.0 0.0 0.0 1.3022037193542632E-4 0.0 10 0.0 0.0 0.0 1.3022037193542632E-4 0.0 11 0.0 0.0 0.0 1.3022037193542632E-4 0.0 12 0.0 0.0 0.0 1.3022037193542632E-4 0.0 13 0.0 0.0 0.0 1.3022037193542632E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTACA 25 8.19145E-4 26.786245 1 CCTACCG 30 0.007489055 18.298212 3 CTCCTAG 45 0.001169423 17.857496 1 GTCTAAG 55 2.2767698E-4 17.045792 1 GTATTGG 50 0.0023668464 16.071747 1 GTCCTAC 830 0.0 15.974929 1 GTCCTAA 425 0.0 15.756614 1 ACAGTCG 40 0.0027821534 15.688082 6 GGACGTT 50 7.241152E-4 14.64221 6 GTCCTAG 55 0.0044650123 14.610678 1 GATATAG 55 0.0044650123 14.610678 1 GTCTTAC 55 0.0044650123 14.610678 1 GTTCTGG 75 1.4735761E-4 14.285997 1 TAGGACC 345 0.0 14.007873 4 AGGACCG 45 0.0060759904 13.948746 5 CTTAGGA 60 0.0067160265 13.738331 2 TCCTACA 950 0.0 13.73833 2 TCCTAAA 505 0.0 13.058214 2 TTTAGAA 245 0.0 12.897207 2 TTAAACT 100 4.3657383E-7 12.887242 4 >>END_MODULE