FastQCFastQC Report
Thu 26 May 2016
SRR1926703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926703_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767929
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA25080.32659269281404923No Hit
CTTTAGGACGTGAAATATGGCGAGG24430.3181283686382465No Hit
GTCCTACAGTGGACATTTCTAAATT24100.31383109636437745No Hit
GTCCTAAAGTGTGTATTTCTCATTT23890.31109646855373346No Hit
GTCCTACAGTGTGCATTTCTCATTT17720.23075049906957543No Hit
GTATCAACGCAGAGTACTTTTTTTT14390.18738711521507848No Hit
CTGTAGGACCTGGAATATGGCGAGA14120.18387116517282195No Hit
CTGAAGGACCTGGAATATGGCGAGA12550.16342656677896003No Hit
GTACATGGGAAGCAGTGGTATCAAC10670.13894513685509988No Hit
CCCATGTACTCTGCGTTGATACCAC9720.1265742015212344No Hit
GAATATGGCAAGAAAACTGAAAATC8320.10834334945027471No Hit
GGTATCAACGCAGAGTACTTTTTTT8270.10769224759059758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC1102.562956E-913.011353
CCTACAC1001.358967E-712.4041533
GGGTGTC701.0843075E-412.2205457
CTAGCAC550.00296713212.1439273
TAGGACA4250.011.8982254
TAGGACC5700.011.7170544
TAGAACT1156.642222E-811.6151674
GGCGAGG6550.011.27897519
GACGTGG6250.011.2537647
ATAGGAC1701.0913936E-1111.2254773
CTAGAGT1201.1976954E-711.1312024
TTAATCC1303.0540832E-811.0088814
TGTAGGA15000.011.0061542
AGGACGT14650.010.9412495
TTAGGAC9550.010.8904763
ACTGACC700.001486788910.8598688
TTTAGAA3700.010.832492
TATGTCG700.001527796210.8245116
GGACGTG14200.010.8162286
TAGAAAT3450.010.7855124
Produced by FastQC (version 0.11.5)