FastQCFastQC Report
Thu 26 May 2016
SRR1926702_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926702_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748460
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14030.187451567218021No Hit
GTCCTAAAGTGTGTATTTCTCATTT12070.1612644630307565No Hit
CTGTAGGACGTGGAATATGGCAAGA10040.13414210512251823No Hit
CTTTAGGACGTGAAATATGGCGAGG9970.1332068514015445No Hit
GTCCTACAGTGTGCATTTCTCATTT8810.11770836116826551No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGA450.001154620417.8912541
GTCCTAC10850.014.7169981
TAGGACC3450.014.702874
CATCTAA1303.1122909E-914.4506271
CCAACGA855.0495146E-913.9662319
AACAGTC602.1738434E-413.6475847
TTTAGGA4850.013.6370012
TCCTACA11850.013.4883552
GTATTAA802.5069088E-413.418441
GACCTAA600.00784877513.418441
CTATATC652.733486E-413.2831154
CTTGCAC500.008606452513.2334123
TGTAGAA2200.013.1527332
CTTTAGG4300.013.1063821
CTGTAGG7600.013.0653231
TTAGGAC4600.012.9457293
TCCAACG1055.329639E-1012.92797418
TAGAACA2600.012.8680184
CCAGAAC600.001922052212.8658183
GACCTGT654.2768015E-412.5977717
Produced by FastQC (version 0.11.5)