##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926702_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 748460 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.456333003767735 27.0 7.0 31.0 2.0 31.0 2 16.451540496486118 16.0 2.0 31.0 2.0 31.0 3 20.157722523581754 25.0 15.0 31.0 2.0 31.0 4 22.144013040109023 28.0 2.0 35.0 2.0 37.0 5 22.971132725863775 30.0 2.0 35.0 2.0 37.0 6 23.415486465542582 30.0 2.0 35.0 2.0 37.0 7 23.373148865670846 30.0 7.0 35.0 2.0 37.0 8 23.557631670363147 31.0 10.0 35.0 2.0 37.0 9 26.429541992892073 32.0 17.0 37.0 2.0 39.0 10 27.19189803062288 32.0 24.0 37.0 2.0 39.0 11 24.247116746385913 30.0 7.0 37.0 2.0 39.0 12 25.4172594393822 31.0 15.0 37.0 2.0 39.0 13 21.948551692809236 28.0 2.0 37.0 2.0 39.0 14 25.42535606445234 31.0 17.0 38.0 2.0 40.0 15 26.562803623440132 31.0 17.0 38.0 2.0 40.0 16 26.947448093418487 31.0 17.0 39.0 2.0 40.0 17 26.275684739331428 31.0 16.0 38.0 2.0 40.0 18 26.398056008337118 31.0 17.0 38.0 2.0 40.0 19 26.7814632712503 31.0 18.0 39.0 2.0 40.0 20 27.03412473612484 31.0 19.0 39.0 2.0 40.0 21 24.880795232878178 30.0 8.0 38.0 2.0 40.0 22 25.82601875851749 31.0 10.0 38.0 2.0 40.0 23 25.827068914838467 31.0 2.0 38.0 2.0 40.0 24 24.80930577452369 31.0 2.0 39.0 2.0 40.0 25 24.615127060898377 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.703446764512481 1101 2 10.493905959057866 1101 3 7.104601947472627 1101 4 8.469672630792079 1101 5 8.223874973922271 1101 6 7.7097263504441 1101 7 7.292979677455264 1101 8 5.957392473953032 1101 9 5.789296206968533 1101 10 5.372029830917448 1101 11 8.101111415478893 1101 12 5.654022341316313 1101 13 10.172482043917167 1101 14 7.812564100199168 1101 15 7.022285143858529 1101 16 6.367981705637259 1101 17 2.2885741797116452 1101 18 4.56669649512034 1101 19 5.354178283968562 1101 20 3.3279872585568597 1101 21 0.497051695283222 1101 22 4.244953976319284 1101 23 5.42236384699844 1101 24 6.872799654121319 1101 25 7.410227273080196 1102 1 5.705505124331296 1102 2 10.518442984508955 1102 3 6.942943747817981 1102 4 7.93081279946497 1102 5 8.173869968792012 1102 6 6.798192653404634 1102 7 6.488134711365024 1102 8 6.624224727012418 1102 9 5.861671641789226 1102 10 5.116286443945796 1102 11 7.6527480930734235 1102 12 5.355075921235731 1102 13 9.518359693508 1102 14 6.9293982301824 1102 15 4.932885384104779 1102 16 5.389850371019275 1102 17 6.266517821944937 1102 18 6.325986642521425 1102 19 5.152798744941059 1102 20 5.431321926591593 1102 21 4.408634817983991 1102 22 5.452716307708961 1102 23 5.897866236948136 1102 24 7.370367160815682 1102 25 6.842312660602406 1103 1 5.0413967663239205 1103 2 9.329737202991112 1103 3 6.3715604430545945 1103 4 7.885267113526577 1103 5 7.145162369186796 1103 6 6.563284077226772 1103 7 6.776311948911125 1103 8 6.5616020061148 1103 9 5.483979573844703 1103 10 4.736916541083609 1103 11 6.043836867682536 1103 12 4.644925799164149 1103 13 8.694463532269623 1103 14 6.981516301970878 1103 15 5.814108437452656 1103 16 5.433996129681159 1103 17 3.6959578510343327 1103 18 2.4370574959835842 1103 19 4.253018214059544 1103 20 4.729599938123464 1103 21 6.787729410472693 1103 22 2.840707840616343 1103 23 5.010540903199242 1103 24 6.172419002278463 1103 25 2.6253992535066715 1104 1 5.6739665472898295 1104 2 10.275131715458176 1104 3 7.0301256092259266 1104 4 7.279602809224972 1104 5 7.6738361841627665 1104 6 7.2170964047497606 1104 7 7.244104580480869 1104 8 7.0233350651089665 1104 9 5.842050279893282 1104 10 4.8832788611734586 1104 11 7.814842990343127 1104 12 4.811508764900488 1104 13 9.215150779371008 1104 14 6.8862646432557995 1104 15 6.261230062400028 1104 16 5.8753517599429195 1104 17 6.328139734405205 1104 18 4.30447772087674 1104 19 4.794054156738149 1104 20 4.112316195717451 1104 21 3.585492346951181 1104 22 5.257366699360404 1104 23 5.611657873375474 1104 24 6.619114626968489 1104 25 5.807434410395945 1105 1 3.5800971546918277 1105 2 9.68156442663419 1105 3 6.3562644309299365 1105 4 5.651255557213229 1105 5 2.2444898296395763 1105 6 4.7144766508745874 1105 7 5.76188550200386 1105 8 3.046103284887181 1105 9 3.7819562717702517 1105 10 3.3781650018205625 1105 11 6.563975164547177 1105 12 4.131850114784118 1105 13 5.284725917416917 1105 14 3.8731521833765825 1105 15 4.191617199411546 1105 16 4.65652336279663 1105 17 4.969952357049298 1105 18 2.303731740560643 1105 19 2.346736126345231 1105 20 1.9133396369015259 1105 21 2.549906165232567 1105 22 3.900556282408516 1105 23 1.3429516259424439 1105 24 4.4155411013596435 1105 25 5.049282932582166 1106 1 6.206248312794063 1106 2 11.066729965812542 1106 3 7.550316668732904 1106 4 8.702158891890587 1106 5 8.239824566733194 1106 6 7.176630974904278 1106 7 6.976663812414085 1106 8 6.732468591101057 1106 9 5.759299580761784 1106 10 4.981118274523396 1106 11 7.488793347722957 1106 12 4.857181417145121 1106 13 4.595142117407956 1106 14 5.186952341672956 1106 15 5.17659039356354 1106 16 5.231251496243459 1106 17 5.836818536879953 1106 18 5.8992349288885215 1106 19 5.588632098747901 1106 20 4.979941192984736 1106 21 5.165676068014701 1106 22 5.448009652179884 1106 23 5.973555989546227 1106 24 6.60373816540994 1106 25 5.97495890289413 1107 1 4.323626566456536 1107 2 -14.167847338071692 1107 3 -5.579747792141109 1107 4 2.15228942530592 1107 5 4.8758623786591855 1107 6 5.111716273996564 1107 7 4.89959876912889 1107 8 5.4811223834356895 1107 9 3.8187171579429027 1107 10 3.171197851323999 1107 11 5.989083389655402 1107 12 3.410291673225675 1107 13 5.398706054926468 1107 14 4.423445027787075 1107 15 4.013883560501437 1107 16 3.7833580966901863 1107 17 4.308592540395367 1107 18 -0.9866374980438906 1107 19 1.8917628643131472 1107 20 2.260515598195134 1107 21 -0.942530809557347 1107 22 2.3351068299002407 1107 23 -1.0184845849055293 1107 24 2.721871633572526 1107 25 3.52296767920809 1108 1 4.9432460983229305 1108 2 10.337368757308337 1108 3 0.4380545597981751 1108 4 5.77287327732661 1108 5 6.96716596417685 1108 6 7.006404998964943 1108 7 7.079582237926246 1108 8 6.80406944601631 1108 9 5.209085227674521 1108 10 4.266771997524952 1108 11 6.680576082112413 1108 12 2.143206299582925 1108 13 2.95857530814191 1108 14 4.525719320514597 1108 15 4.506231617458944 1108 16 4.474262178143931 1108 17 0.1700873618709018 1108 18 3.400092454741994 1108 19 4.124558516105903 1108 20 4.465245776406508 1108 21 4.64977466702971 1108 22 1.9349515367844852 1108 23 4.431427123443978 1108 24 5.145007803092497 1108 25 5.744899074717111 1109 1 4.017744213515357 1109 2 -14.167847338071692 1109 3 -5.6978532161288875 1109 4 2.3689026160367597 1109 5 1.6421368884631065 1109 6 3.057221748913804 1109 7 3.9940423647489567 1109 8 4.2123777946911005 1109 9 3.676073918829072 1109 10 2.7491453939774253 1109 11 1.4384849684687424 1109 12 1.911457957921371 1109 13 3.13649062329927 1109 14 3.559426693180505 1109 15 3.801028964117428 1109 16 3.785935127502512 1109 17 4.099364121755183 1109 18 4.3586337013449565 1109 19 2.876147891051115 1109 20 1.5454964996466245 1109 21 1.0957885181737446 1109 22 3.2927131451536162 1109 23 3.661382998491465 1109 24 4.750443062143955 1109 25 5.0500672463076555 1110 1 3.138350604219788 1110 2 9.187090933533245 1110 3 6.339220131728776 1110 4 7.533515905797106 1110 5 8.262345675681697 1110 6 3.7486705506567226 1110 7 6.281847375642204 1110 8 7.1583545557659 1110 9 5.415977695154417 1110 10 5.076187877637558 1110 11 3.4231981129858085 1110 12 5.784720485154487 1110 13 10.269275663241174 1110 14 7.670492415765835 1110 15 6.994160772396295 1110 16 6.487236870421906 1110 17 7.120072182757362 1110 18 7.115029852397971 1110 19 6.135231042830341 1110 20 6.141905359486856 1110 21 4.249987864578973 1110 22 6.207186638254559 1110 23 6.952167312216957 1110 24 7.906752067227469 1110 25 8.115938706002648 1111 1 5.167699650235502 1111 2 9.739728419504065 1111 3 7.474517550359526 1111 4 8.175729710510911 1111 5 8.274480916983606 1111 6 7.4904213924075655 1111 7 7.316653772948602 1111 8 7.462030201108213 1111 9 5.899745933089324 1111 10 5.073788887738566 1111 11 7.113574095028458 1111 12 6.370789508723512 1111 13 8.076570837203015 1111 14 6.003811719918474 1111 15 5.454219694955217 1111 16 5.06042710611214 1111 17 6.198775886461064 1111 18 6.035077551612336 1111 19 5.120907249339883 1111 20 4.637724663639496 1111 21 7.447588874679987 1111 22 6.781821879556464 1111 23 6.730197615247256 1111 24 6.936227375869446 1111 25 7.553480793544733 1112 1 -2.232731306033781 1112 2 8.04847002719777 1112 3 5.914809693346282 1112 4 -0.04766894672007993 1112 5 4.361420673833543 1112 6 3.555445888869187 1112 7 5.818205215518006 1112 8 6.032670738215817 1112 9 4.790196849438445 1112 10 3.472936047963568 1112 11 -0.6689971261655181 1112 12 3.9214608932272306 1112 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3.627903354889529 2204 7 4.943024624319452 2204 8 4.899800763878776 2204 9 3.6053736387170296 2204 10 3.1013862903359666 2204 11 4.56014696660133 2204 12 4.86454852748253 2204 13 2.6137641993963747 2204 14 4.127260493367245 2204 15 3.986687227422749 2204 16 4.075001420686455 2204 17 -1.1996368119516347 2204 18 2.3954310869658713 2204 19 3.0867081151407483 2204 20 3.538951214866046 2204 21 5.108122785213894 2204 22 4.34625189398648 2204 23 3.0434371534867957 2204 24 4.9469416615837325 2204 25 5.160515425579366 2205 1 6.041693467382924 2205 2 10.439600295752978 2205 3 7.462243530094817 2205 4 7.77456014048952 2205 5 7.9833784650006265 2205 6 7.466126660762175 2205 7 7.672343525399409 2205 8 7.314258261385149 2205 9 6.09806269261632 2205 10 5.346428589645143 2205 11 7.656580771568883 2205 12 6.802199222619649 2205 13 10.004134487672015 2205 14 7.36570607071895 2205 15 6.345791738117644 2205 16 5.798548036126011 2205 17 6.698194040068515 2205 18 6.635742267036552 2205 19 5.254876499639622 2205 20 5.486249477331107 2205 21 -0.6984486926143738 2205 22 4.081261191214555 2205 23 5.4316733892792755 2205 24 7.39646140741845 2205 25 7.732958072149241 2206 1 4.127026078179782 2206 2 8.834501917997219 2206 3 6.043433807686121 2206 4 1.588601972678397 2206 5 5.4960199170073345 2206 6 5.7931070192317975 2206 7 6.123959603375027 2206 8 6.10669750848108 2206 9 4.7475521075987075 2206 10 3.881139804897625 2206 11 3.9115944315178623 2206 12 4.87299341940432 2206 13 7.999830252024683 2206 14 0.05783749054565135 2206 15 2.0153928991981154 2206 16 3.239370415478316 2206 17 4.3144767478549575 2206 18 -1.4441190740016765 2206 19 0.8863482687746398 2206 20 2.392977267991551 2206 21 3.1540979750810187 2206 22 -2.4972590629935425 2206 23 -3.1738441730349365 2206 24 0.9590477882943986 2206 25 2.744803377251813 2207 1 1.9262306120273323 2207 2 -14.167847338071692 2207 3 -6.124033174278155 2207 4 -4.1321095516761766 2207 5 1.7282687957714842 2207 6 2.664630878573572 2207 7 3.937503575320143 2207 8 3.981364851964603 2207 9 1.4931583178060563 2207 10 1.1888415886608321 2207 11 -0.9581979734827399 2207 12 -2.1291775532000727 2207 13 1.1480135249500485 2207 14 -4.837690256366113 2207 15 -0.8200886054400378 2207 16 0.44744950400410133 2207 17 1.5440328034571102 2207 18 1.4025676701894092 2207 19 0.9068756287865227 2207 20 0.6615718309649239 2207 21 -3.2831094125400426 2207 22 -3.905727527558156 2207 23 -0.6936166140017548 2207 24 1.1030587329181927 2207 25 1.772683692095459 2208 1 5.337773997580882 2208 2 6.955756533856828 2208 3 6.685020946194271 2208 4 8.64582492926585 2208 5 4.286882482903412 2208 6 6.571493280311174 2208 7 7.1100115878812495 2208 8 7.095902242444886 2208 9 5.2638922360293705 2208 10 4.969497838752801 2208 11 6.721036470082048 2208 12 6.507099556332889 2208 13 9.034837607662634 2208 14 3.5795309374098423 2208 15 5.107993471439343 2208 16 5.576146097966646 2208 17 6.2503395499011205 2208 18 0.890338839096259 2208 19 3.3771754413117243 2208 20 4.4717213693413385 2208 21 7.318186135448503 2208 22 6.367907982582256 2208 23 2.2407624971263616 2208 24 3.589758028636883 2208 25 4.887783801284076 2209 1 3.054719003431323 2209 2 4.993344411355352 2209 3 5.291146019929172 2209 4 0.5235652083464117 2209 5 5.069903631523967 2209 6 5.554802599435831 2209 7 5.978284788991381 2209 8 4.705721313916975 2209 9 4.332850083840533 2209 10 3.5050404792842755 2209 11 -0.4249731420481915 2209 12 3.126869213332629 2209 13 2.05187641397918 2209 14 3.5082425831728656 2209 15 3.959643682732146 2209 16 3.522089953069102 2209 17 1.2215031591883374 2209 18 3.424363265898048 2209 19 3.9100974708830485 2209 20 3.9832046738253872 2209 21 6.201090275240208 2209 22 4.734648464735994 2209 23 0.39557194679806074 2209 24 -3.1314515138682673 2209 25 0.23572550961297978 2210 1 -1.9876479433473868 2210 2 -14.167847338071692 2210 3 -7.664683281488363 2210 4 -2.08590130553187 2210 5 -0.004039582125130892 2210 6 -0.8300331530469798 2210 7 -0.8372975045320885 2210 8 -21.26448495040694 2210 9 -13.136899937380077 2210 10 -7.022259834780744 2210 11 -10.027691502119492 2210 12 -4.454522434235489 2210 13 -5.331123755785697 2210 14 -3.8335471630286477 2210 15 -5.6339710358825705 2210 16 -4.968509316941933 2210 17 -4.488315616806911 2210 18 -4.799503553557006 2210 19 -10.014505703720129 2210 20 -7.1680982715952055 2210 21 -5.489799717120373 2210 22 -10.434607116284294 2210 23 -8.213682360985658 2210 24 -10.870798767921404 2210 25 -8.549638636045286 2211 1 -0.03558911981797408 2211 2 4.886270308987132 2211 3 0.6252840387730743 2211 4 1.5085104591740155 2211 5 0.9527251237572223 2211 6 -0.09258217265482216 2211 7 -21.053800772505944 2211 8 -13.478602597465763 2211 9 -9.201573139994455 2211 10 -7.202227155042184 2211 11 -10.007005227609687 2211 12 -4.940306747960982 2211 13 -7.267430945328181 2211 14 -4.983318404858711 2211 15 -5.502500447647279 2211 16 -11.70504526465435 2211 17 -7.9900476429506995 2211 18 -6.064111396694258 2211 19 -8.69561681483124 2211 20 -6.87254271603965 2211 21 -4.536368344571354 2211 22 -6.202188815630699 2211 23 -8.286067981900693 2211 24 -22.55543929079722 2211 25 -22.360913145849207 2212 1 -1.3135743406569027 2212 2 -14.167847338071692 2212 3 -8.214963411898776 2212 4 -19.853058168276966 2212 5 -20.6535493860467 2212 6 -21.08003315304698 2212 7 -10.337829096660153 2212 8 -9.878174958274759 2212 9 -5.612325211082528 2212 10 -5.858339834575052 2212 11 -6.088125235014694 2212 12 -3.359007477662775 2212 13 -7.020054940638342 2212 14 -4.236750471627197 2212 15 -4.363790461685937 2212 16 -4.4855626895629435 2212 17 -4.763261879077881 2212 18 -3.6647883378090214 2212 19 -4.326095826262719 2212 20 -4.4422815352494744 2212 21 -2.2117821765085353 2212 22 -3.5982475003162158 2212 23 -3.7688528818559526 2212 24 -2.6797508564935235 2212 25 -3.2602050419939737 2213 1 -19.205785198249348 2213 2 -14.167847338071692 2213 3 -8.880977777708203 2213 4 -6.2959883215989425 2213 5 -20.6535493860467 2213 6 -10.881566372808479 2213 7 -21.053800772505944 2213 8 -10.93058375790268 2213 9 -8.948305277483183 2213 10 -7.618079110815977 2213 11 -6.726438036508373 2213 12 -5.559563854551488 2213 13 -19.62115643552426 2213 14 -12.826233192579592 2213 15 -7.468143631667054 2213 16 -6.731408627716782 2213 17 -5.420900433811507 2213 18 -5.342965657308216 2213 19 -6.282972261268757 2213 20 -6.173637682135116 2213 21 -4.350795307860928 2213 22 -5.30300853704567 2213 23 -5.145646429974981 2213 24 -3.9847408240169813 2213 25 -4.9094651049633455 2214 1 -19.205785198249348 2214 2 -14.167847338071692 2214 3 -12.376009632069497 2214 4 -19.853058168276966 2214 5 -20.6535493860467 2214 6 -21.08003315304698 2214 7 -16.744423033680352 2214 8 -15.024344722536648 2214 9 -13.754778274809118 2214 10 -13.788374153718955 2214 11 -14.333445398770177 2214 12 -12.209499578467417 2214 13 -19.62115643552426 2214 14 -16.980038172520377 2214 15 -24.327598486862964 2214 16 -18.43270624315618 2214 17 -24.051224113538936 2214 18 -18.047391972728356 2214 19 -14.925547216439735 2214 20 -13.667130195203093 2214 21 -11.107453220239412 2214 22 -12.225050082836967 2214 23 -12.196459623219797 2214 24 -10.973493629096957 2214 25 -10.738651971440794 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 130963.0 3 3.0 4 566.0 5 2340.0 6 3279.0 7 3131.0 8 2608.0 9 2527.0 10 2254.0 11 2161.0 12 2121.0 13 2303.0 14 3412.0 15 7710.0 16 11557.0 17 12397.0 18 12755.0 19 14726.0 20 17215.0 21 19962.0 22 20074.0 23 21993.0 24 23158.0 25 23684.0 26 19616.0 27 15294.0 28 15826.0 29 18718.0 30 18338.0 31 21068.0 32 27388.0 33 34420.0 34 42668.0 35 49541.0 36 49456.0 37 52177.0 38 41051.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.51742527595188 19.68219025176733 13.081824382984001 25.718560089296787 2 12.738181353984936 21.023998978766862 43.50282966682269 22.734990000425515 3 18.009389911166164 25.650291785317513 30.343544755313218 25.996773548203105 4 11.436596610084903 17.645306583322416 39.69566248073912 31.22243432585356 5 11.497652063335922 38.28016168621854 37.806216234304934 12.415970016140605 6 29.47307323075366 38.50992188698522 19.866548366332484 12.150456515928635 7 26.10427339054842 31.36362628345863 23.84863873919825 18.683461586794696 8 22.74599236724331 39.552428386654725 21.987507131212865 15.714072114889102 9 26.29182461950866 15.195709925011972 21.151011388656286 37.36145406682308 10 14.219907137294063 29.757178406571487 35.99393217386113 20.02898228227332 11 32.341867095838424 21.84717000679806 27.110200918802235 18.70076197856128 12 23.968676505529558 24.550585537070734 33.276233213204925 18.204504744194782 13 28.391785211063713 22.42676626326185 25.94658585702613 23.234862668648304 14 19.09538588643269 23.897564395936495 28.495371754385012 28.51167796324581 15 21.054766118697398 34.54206607910677 23.5129717940901 20.890196008105733 16 19.784728182478204 28.2228433899594 31.112054037000963 20.880374390561435 17 19.803741733499756 29.632929461496555 30.40058477515809 20.1627440298456 18 19.754006658303055 27.243511413070042 33.793603467096794 19.20887846153011 19 22.705844996458318 26.706724310593295 27.96436063820837 22.62307005474002 20 22.84799228135532 28.485684996214378 29.390749686765073 19.275573035665232 21 21.064191910348452 26.68884608649974 27.229697075818592 25.017264927333215 22 22.02565224599846 30.15334813723598 28.276178853612592 19.54482076315296 23 20.225500648090307 29.443899724578337 29.786605063531578 20.543994563799775 24 22.41814122400433 27.45841947522434 30.19090666277091 19.932532638000417 25 21.39126921914169 28.291566452013345 29.86160345228891 20.45556087655606 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 810.0 1 810.0 2 1740.5 3 2671.0 4 2671.0 5 2671.0 6 3837.0 7 5003.0 8 5003.0 9 5003.0 10 5851.5 11 6700.0 12 6700.0 13 6700.0 14 8521.5 15 10343.0 16 10343.0 17 10343.0 18 15212.5 19 20082.0 20 20082.0 21 20082.0 22 27917.5 23 35753.0 24 35753.0 25 35753.0 26 47235.0 27 58717.0 28 58717.0 29 58717.0 30 70193.0 31 81669.0 32 81669.0 33 81669.0 34 87119.0 35 92569.0 36 92569.0 37 92569.0 38 93452.0 39 94335.0 40 94335.0 41 94335.0 42 92516.5 43 90698.0 44 90698.0 45 90698.0 46 87190.5 47 83683.0 48 83683.0 49 83683.0 50 74547.5 51 65412.0 52 65412.0 53 65412.0 54 54643.0 55 43874.0 56 43874.0 57 43874.0 58 35700.0 59 27526.0 60 27526.0 61 27526.0 62 21542.0 63 15558.0 64 15558.0 65 15558.0 66 11648.0 67 7738.0 68 7738.0 69 7738.0 70 5565.0 71 3392.0 72 3392.0 73 3392.0 74 2343.0 75 1294.0 76 1294.0 77 1294.0 78 858.5 79 423.0 80 423.0 81 423.0 82 287.0 83 151.0 84 151.0 85 151.0 86 97.5 87 44.0 88 44.0 89 44.0 90 28.5 91 13.0 92 13.0 93 13.0 94 7.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.817705689014778 2 37.20172086684659 3 5.83344467306202 4 14.331293589503783 5 14.159874943216739 6 15.521337145605644 7 9.628437057424579 8 7.961280495951688 9 0.11677310744729179 10 1.5308767335595757 11 5.858295700505037 12 6.092643561446169 13 16.93503994869465 14 1.675974667984929 15 3.1452582636346635 16 3.017796542233386 17 3.1257515431686396 18 1.594741201934639 19 1.539427624722764 20 0.29594099885097397 21 4.620821419982364 22 1.6858616358923657 23 0.11744114581941587 24 9.459289741602758 25 0.11997969163348743 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 748460.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.34704666542196 #Duplication Level Percentage of deduplicated Percentage of total 1 96.32813581721788 86.0663444605407 2 2.530492918008756 4.521841376636962 3 0.4811671833372202 1.2897260035050075 4 0.21072225350467705 0.7530964406930102 5 0.11705486302006576 0.5229253154334196 6 0.07092104110143815 0.3801951341310302 7 0.045325616140911296 0.2834796958336703 8 0.03259548972960819 0.23298485935628685 9 0.023084836304188807 0.18563057518805862 >10 0.13705381313975507 2.336808122391778 >50 0.012817522869464611 0.8322215761678569 >100 0.00969082395714489 1.7502668008708016 >500 4.6891083447687926E-4 0.3397749234825135 >1k 4.6891083447687926E-4 0.5047047157689287 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1403 0.187451567218021 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1207 0.1612644630307565 No Hit CTGTAGGACGTGGAATATGGCAAGA 1004 0.13414210512251823 No Hit CTTTAGGACGTGAAATATGGCGAGG 997 0.1332068514015445 No Hit GTCCTACAGTGTGCATTTCTCATTT 881 0.11770836116826551 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTAGA 45 0.0011546204 17.891254 1 GTCCTAC 1085 0.0 14.716998 1 TAGGACC 345 0.0 14.70287 4 CATCTAA 130 3.1122909E-9 14.450627 1 CCAACGA 85 5.0495146E-9 13.96623 19 AACAGTC 60 2.1738434E-4 13.647584 7 TTTAGGA 485 0.0 13.637001 2 TCCTACA 1185 0.0 13.488355 2 GTATTAA 80 2.5069088E-4 13.41844 1 GACCTAA 60 0.007848775 13.41844 1 CTATATC 65 2.733486E-4 13.283115 4 CTTGCAC 50 0.0086064525 13.233412 3 TGTAGAA 220 0.0 13.152733 2 CTTTAGG 430 0.0 13.106382 1 CTGTAGG 760 0.0 13.065323 1 TTAGGAC 460 0.0 12.945729 3 TCCAACG 105 5.329639E-10 12.927974 18 TAGAACA 260 0.0 12.868018 4 CCAGAAC 60 0.0019220522 12.865818 3 GACCTGT 65 4.2768015E-4 12.597771 7 >>END_MODULE