FastQCFastQC Report
Thu 26 May 2016
SRR1926702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926702_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748460
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA29460.3936082088555166No Hit
GTCCTACAGTGGACATTTCTAAATT28430.3798466183897603No Hit
CTTTAGGACGTGAAATATGGCGAGG27660.3695588274590492No Hit
GTCCTAAAGTGTGTATTTCTCATTT26540.3545947679234695No Hit
GTCCTACAGTGTGCATTTCTCATTT18920.2527857200117575No Hit
CTGTAGGACCTGGAATATGGCGAGA15890.21230259466103732No Hit
CTGAAGGACCTGGAATATGGCGAGA15600.2084279721027176No Hit
GTATCAACGCAGAGTACTTTTTTTT11700.15632097907703818No Hit
CCCATGTACTCTGCGTTGATACCAC10620.14189135023915772No Hit
GAATATGGCAAGAAAACTGAAAATC9640.12879779814552547No Hit
GTCCTTCAGTGTGCATTTCTCATTT9570.12786254442455175No Hit
GTACATGGGAAGCAGTGGTATCAAC9170.12251823744755899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGT350.002170618616.2836468
GGCGAAA350.002216700516.23251719
TTAGGGT551.879146E-413.8852864
AATGGCG500.001535673613.256556516
TAGGACC5750.012.9495384
CTAGGAC1650.012.7281793
CAGTACT853.7279842E-612.3538214
GGTCTAC701.044526E-412.2694691
CGCCACC701.114152E-412.18497114
CCCTATA550.002949143812.1537182
TAGACAG959.783744E-712.0582745
TAAGACC802.7232127E-511.9326684
TGTAGGA16800.011.8798462
GTCTTAT657.7173114E-411.7451321
CTGTAGG16900.011.5192651
TATTAAT1001.8085921E-611.4592212
TAGGACA4750.011.4553624
TGGCGAA752.1349663E-411.36276318
GTCTTAG1104.673766E-711.2779971
GTAGGAC17550.011.2595433
Produced by FastQC (version 0.11.5)