FastQCFastQC Report
Thu 26 May 2016
SRR1926701_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926701_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631393
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12020.1903727155670082No Hit
GTCCTAAAGTGTGTATTTCTCATTT10360.16408164170334483No Hit
CTGTAGGACGTGGAATATGGCAAGA8580.13589000828327205No Hit
CTTTAGGACGTGAAATATGGCGAGG7750.12274447135144038No Hit
GTCCTACAGTGTGCATTTCTCATTT6910.10944055445657459No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACA404.3538076E-420.6805632
GAGTAAC450.001148764517.9036981
TAAACTA300.00976916517.4726335
GTCTAAG552.2308281E-417.0898931
GTCCTAC8500.015.007511
GATATAC1750.014.5787251
TCCTACA9800.013.9277282
TGTTAGA600.006557237413.7870442
TACTATG600.006557237413.7870442
ACTGACC500.001200913913.6943058
AACTGCC450.00703852913.641747
TTACTAA600.007809601713.4277731
AAATGTC1850.013.2730447
CTACATC500.008645697513.22363953
TTTAGGA5150.013.1177692
TAGGACC3050.013.07041554
TGTAGGA7000.012.9992132
GCAGGAC600.001931912712.8563173
TCCTATA1101.6391785E-512.5336762
AATGTCC1800.012.4987718
Produced by FastQC (version 0.11.5)