##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926701_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 631393 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.09489493865152 27.0 2.0 31.0 2.0 31.0 2 16.199368697467346 16.0 2.0 30.0 2.0 31.0 3 19.819801613258303 23.0 15.0 31.0 2.0 31.0 4 21.778057406401402 28.0 2.0 35.0 2.0 37.0 5 22.589141786494306 30.0 2.0 35.0 2.0 37.0 6 23.024434860696903 30.0 2.0 35.0 2.0 37.0 7 22.98343028826737 30.0 2.0 35.0 2.0 37.0 8 23.16149212930774 30.0 7.0 35.0 2.0 37.0 9 25.948526512013913 31.0 17.0 37.0 2.0 39.0 10 26.682471931111053 31.0 22.0 37.0 2.0 39.0 11 23.76910260329145 30.0 7.0 37.0 2.0 39.0 12 24.937298956434425 31.0 10.0 37.0 2.0 39.0 13 21.557261483735168 27.0 2.0 35.0 2.0 39.0 14 24.919655428552424 30.0 13.0 38.0 2.0 40.0 15 26.032149548696296 31.0 17.0 38.0 2.0 40.0 16 26.410821786114195 31.0 16.0 39.0 2.0 40.0 17 25.758679617924177 31.0 10.0 38.0 2.0 40.0 18 25.84605942732973 31.0 11.0 38.0 2.0 40.0 19 26.231241081228333 31.0 13.0 39.0 2.0 40.0 20 26.480041748958257 31.0 11.0 39.0 2.0 40.0 21 24.366939449756334 30.0 2.0 38.0 2.0 40.0 22 25.28935068966555 31.0 2.0 38.0 2.0 40.0 23 25.28173261344361 31.0 2.0 38.0 2.0 40.0 24 24.31038513255611 31.0 2.0 39.0 2.0 40.0 25 24.125097997602126 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.06020829260552 1101 2 10.71844899890064 1101 3 7.341050188667239 1101 4 8.993181124950738 1101 5 8.626898688148806 1101 6 8.18786181807495 1101 7 7.825204036725719 1101 8 6.525315266027363 1101 9 6.602298528993714 1101 10 6.092754913491511 1101 11 9.073608194905685 1101 12 6.477753843011396 1101 13 10.781065809306966 1101 14 8.264059442802491 1101 15 7.607195273801665 1101 16 6.990806263085997 1101 17 2.951002228213479 1101 18 5.346985545356933 1101 19 6.264859817117149 1101 20 4.278437255165976 1101 21 1.3157048534564382 1101 22 5.405066355430044 1101 23 6.496495711613946 1101 24 7.970363069385897 1101 25 8.374936570604397 1102 1 6.082034749888599 1102 2 10.965309095606706 1102 3 7.275831337698612 1102 4 8.584103359915506 1102 5 8.79733216445528 1102 6 7.282336000097576 1102 7 7.044540236683996 1102 8 7.335417925578497 1102 9 6.452821316513422 1102 10 5.7995221000361 1102 11 8.418783142286735 1102 12 5.908506766261588 1102 13 10.386211051936062 1102 14 7.953625984401228 1102 15 6.164513000949462 1102 16 6.341039433750449 1102 17 7.1929921063953515 1102 18 7.172268246322826 1102 19 5.979993215446413 1102 20 6.320128653411082 1102 21 5.143396701122452 1102 22 6.380005321027859 1102 23 7.086984640371778 1102 24 8.243206319075014 1102 25 8.112668140990245 1103 1 4.984734558236994 1103 2 9.10777043769587 1103 3 6.3284087360068355 1103 4 7.60806405295336 1103 5 7.42175723320193 1103 6 6.547183814769625 1103 7 6.7946521827161135 1103 8 6.275840011611496 1103 9 5.285176595039864 1103 10 4.407714758450343 1103 11 5.409466404304553 1103 12 4.2426687359084205 1103 13 8.458434389690673 1103 14 6.558137385927843 1103 15 5.49191861512546 1103 16 5.411562134520359 1103 17 3.030651977112999 1103 18 1.594484372396213 1103 19 3.766286119057817 1103 20 4.604297174846746 1103 21 6.470141480195188 1103 22 2.055864288824168 1103 23 4.503909372101951 1103 24 6.231452692730603 1103 25 2.2304579916565928 1104 1 5.839749687448638 1104 2 10.381910265810184 1104 3 7.130314570604035 1104 4 7.559514896025888 1104 5 7.877939207508291 1104 6 7.2906349477877335 1104 7 6.943711160620772 1104 8 7.135678274019682 1104 9 6.0909260671902175 1104 10 5.05054548227346 1104 11 7.878726597006846 1104 12 5.046262202468032 1104 13 9.119555847150451 1104 14 7.1791357652064 1104 15 6.334907507979416 1104 16 6.039518278392844 1104 17 6.40736748845805 1104 18 4.0440152553176745 1104 19 4.716585543627357 1104 20 4.242239713580503 1104 21 3.2582256461927486 1104 22 5.230749797492798 1104 23 5.3524936570515464 1104 24 6.569809890815478 1104 25 5.636023088156431 1105 1 3.9174037970376787 1105 2 9.731474400305824 1105 3 6.693551390861405 1105 4 5.423851757794257 1105 5 2.451976671186163 1105 6 4.868408401502968 1105 7 6.017406158545221 1105 8 3.755024994650647 1105 9 4.194573846351695 1105 10 3.624992867080433 1105 11 7.007934774673927 1105 12 4.29142098204462 1105 13 5.681264543705041 1105 14 4.00394377682948 1105 15 4.363939263894732 1105 16 4.6792469002276285 1105 17 5.537073798130113 1105 18 3.1405179535327044 1105 19 2.6114355851383593 1105 20 1.8778431797490356 1105 21 3.033747128135463 1105 22 4.399357103428869 1105 23 2.1861253507003617 1105 24 4.997741605219794 1105 25 5.691102996832228 1106 1 6.287049762187056 1106 2 11.021385738021081 1106 3 7.443110127065509 1106 4 8.918845307766613 1106 5 8.529766052188272 1106 6 7.411275489980966 1106 7 7.196397497079531 1106 8 7.046242718917085 1106 9 6.009177527986523 1106 10 5.328369405360835 1106 11 7.9581446565258 1106 12 5.421437158304421 1106 13 4.855837101396954 1106 14 5.801412908840046 1106 15 5.856549375080924 1106 16 5.6167901563653935 1106 17 6.1639427704257095 1106 18 6.215903419470415 1106 19 5.9030587397137815 1106 20 5.21283099636349 1106 21 5.545740084295751 1106 22 5.8664373909158165 1106 23 6.322915079799202 1106 24 7.140731551367182 1106 25 6.171206197274518 1107 1 4.401742930691881 1107 2 -13.905968522068605 1107 3 -5.502783299993835 1107 4 2.2305529795639707 1107 5 4.9126085359276175 1107 6 5.226942277845065 1107 7 5.097524633184246 1107 8 5.697169879261285 1107 9 4.167287656051808 1107 10 3.6085051464831253 1107 11 6.299703255483504 1107 12 3.5227056914115202 1107 13 5.174945896290502 1107 14 4.406091129661764 1107 15 4.184610620182529 1107 16 4.07431045693502 1107 17 4.735469454060016 1107 18 -0.17115673485431415 1107 19 2.1919489752407877 1107 20 2.498008550599341 1107 21 -0.7762491695320648 1107 22 2.3219660137090123 1107 23 -0.6581175223096345 1107 24 2.8793410128465986 1107 25 3.5676672323005754 1108 1 5.242025271595342 1108 2 10.496870874559612 1108 3 0.33473398659177533 1108 4 5.885314484636037 1108 5 7.319036347945492 1108 6 7.270249471462815 1108 7 7.626563698869273 1108 8 6.979809750721035 1108 9 5.727540100155181 1108 10 4.750302311882773 1108 11 7.012171180373787 1108 12 2.7550845654053013 1108 13 3.5696739872918855 1108 14 4.928952589169185 1108 15 5.045941427476066 1108 16 4.8162275292868735 1108 17 -0.003002291865147555 1108 18 3.370764334395947 1108 19 4.622487677208063 1108 20 5.060113157424443 1108 21 5.036759935402504 1108 22 2.0934753224854035 1108 23 4.810999243080424 1108 24 5.807940419706874 1108 25 6.066350653965362 1109 1 3.6947071226251467 1109 2 -13.905968522068605 1109 3 -5.689448784597465 1109 4 2.0708199569443515 1109 5 1.6393907070396878 1109 6 2.7362476469442463 1109 7 3.729490995047513 1109 8 4.152596815100594 1109 9 3.4174451237350496 1109 10 2.585095232351563 1109 11 0.775809539837212 1109 12 1.6968987223055834 1109 13 2.954265703445362 1109 14 3.492182469361353 1109 15 3.612145089985038 1109 16 3.4836470662716295 1109 17 4.0229758920819165 1109 18 4.0667815783417325 1109 19 2.3208791237585658 1109 20 1.154210582059104 1109 21 0.6267921097978721 1109 22 2.833178725952653 1109 23 3.149266150022491 1109 24 4.247781618400605 1109 25 4.558918277159865 1110 1 3.3819576029758345 1110 2 9.154195561796481 1110 3 6.458798245088165 1110 4 7.646451375681146 1110 5 8.30026611146122 1110 6 4.187396259629949 1110 7 6.782818678368901 1110 8 7.369218956597511 1110 9 5.6095495168186815 1110 10 5.088827401281222 1110 11 3.042618823740913 1110 12 5.6233232643171505 1110 13 10.197458563926233 1110 14 7.628116801641859 1110 15 6.7082656334455635 1110 16 6.63488598232367 1110 17 7.175994391029803 1110 18 7.008708910189089 1110 19 6.149918704313528 1110 20 6.268591713525254 1110 21 4.4545194295136525 1110 22 6.316476020525311 1110 23 6.714340532828459 1110 24 7.551017246965859 1110 25 7.804987688581832 1111 1 5.557613162625945 1111 2 9.631265520484586 1111 3 7.811770083176569 1111 4 8.556976211306353 1111 5 8.629692108862255 1111 6 7.94938333592442 1111 7 7.9295066151662255 1111 8 8.166122342362687 1111 9 6.49046668614573 1111 10 5.846745514510729 1111 11 7.736036397135795 1111 12 7.184680964557007 1111 13 8.807478961270373 1111 14 6.895649304858239 1111 15 6.377662182383027 1111 16 6.278134493737777 1111 17 7.391933897226586 1111 18 7.176591012893436 1111 19 6.095312977540964 1111 20 5.657314665224604 1111 21 8.244021100738202 1111 22 7.721884549265848 1111 23 7.511443528102479 1111 24 7.211452379532432 1111 25 7.993735854113876 1112 1 -2.4799734114392464 1112 2 7.463733912557185 1112 3 5.639746696188098 1112 4 -0.5614120242134746 1112 5 3.818987141765195 1112 6 3.0969997112929377 1112 7 5.680708899506474 1112 8 5.790548379492808 1112 9 4.29610511192066 1112 10 3.0531131449234046 1112 11 -0.5318859532021882 1112 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5.931396304091507 2204 5 1.4013286470161503 2204 6 4.414075651820543 2204 7 5.461774785592294 2204 8 5.279510866865433 2204 9 3.8491426313278367 2204 10 3.6162423950098486 2204 11 4.960707116487363 2204 12 5.180048372438414 2204 13 2.9452635823976614 2204 14 4.3459305931854395 2204 15 4.601013112900809 2204 16 4.586005234419272 2204 17 -0.7095298685708862 2204 18 2.9363027923420546 2204 19 3.6414189339212726 2204 20 3.97972974547843 2204 21 5.457053682191837 2204 22 4.960699672179516 2204 23 4.164032447208758 2204 24 5.54367922323744 2204 25 5.806292511715327 2205 1 5.8827503686475495 2205 2 10.18122493842186 2205 3 7.034539035966102 2205 4 7.8112953022681495 2205 5 7.56979733235347 2205 6 7.335973379791696 2205 7 7.16452023914443 2205 8 6.836144276001352 2205 9 5.776485682416045 2205 10 5.301177655504407 2205 11 7.422235597089415 2205 12 6.691321446130818 2205 13 9.751922600515162 2205 14 7.040413735646496 2205 15 6.0834470588009815 2205 16 5.444013675139601 2205 17 6.290650247932909 2205 18 6.353248596907964 2205 19 5.140180312845423 2205 20 5.467265000509741 2205 21 -0.9564784451291892 2205 22 3.751980496857012 2205 23 5.177366190285795 2205 24 7.03300966787765 2205 25 7.346683851099208 2206 1 4.398840942780755 2206 2 8.823464750326277 2206 3 6.206588045757766 2206 4 2.0590474703985002 2206 5 6.016614003918463 2206 6 6.279318053986838 2206 7 6.266648601759766 2206 8 6.227250416837254 2206 9 5.050045368059976 2206 10 4.0921654063773865 2206 11 3.8164135672578006 2206 12 4.76121220523796 2206 13 8.176573117661782 2206 14 0.6215316158517936 2206 15 2.640115668192145 2206 16 3.5995686218641154 2206 17 4.588309680026384 2206 18 -1.123835556271711 2206 19 1.162296653815158 2206 20 2.865205546498345 2206 21 3.901394653449966 2206 22 -1.5481197942195735 2206 23 -2.417828970503713 2206 24 1.7034347592438195 2206 25 2.9822677560418676 2207 1 1.2462286251775758 2207 2 -13.905968522068605 2207 3 -6.2755087727193075 2207 4 -4.656082007071014 2207 5 0.8248152481343318 2207 6 1.6729921738949614 2207 7 3.1740121096717218 2207 8 3.2118416939331027 2207 9 0.5843701231342742 2207 10 0.3724839604619383 2207 11 -1.3359961802159326 2207 12 -2.4495544808486507 2207 13 0.038959360303966406 2207 14 -5.840448816878183 2207 15 -1.6503364147645065 2207 16 -0.3532660206414562 2207 17 0.7313559999465618 2207 18 0.645455481757903 2207 19 0.3577048409966537 2207 20 0.25744326003404794 2207 21 -3.4519495952324917 2207 22 -4.161649469906472 2207 23 -1.228872893065006 2207 24 0.653497037002623 2207 25 1.1055743702077123 2208 1 5.2789391208108825 2208 2 6.729764348703295 2208 3 6.569352061733895 2208 4 8.565709211250994 2208 5 3.9269393454882646 2208 6 6.6316007090429565 2208 7 7.066901816091349 2208 8 7.090109566563505 2208 9 5.443161014232192 2208 10 4.952805561381979 2208 11 7.012621377421574 2208 12 6.514150648944941 2208 13 9.310649075619008 2208 14 3.725380410269345 2208 15 5.066501965126779 2208 16 5.399755242570613 2208 17 6.433848144979905 2208 18 1.218366092396181 2208 19 3.696226105797191 2208 20 4.815296002839091 2208 21 7.258403870324791 2208 22 6.514972116081985 2208 23 2.11249966903609 2208 24 3.678017399554207 2208 25 5.082268390266865 2209 1 3.2447559063541114 2209 2 4.959703119722441 2209 3 5.47902556110607 2209 4 0.7352272433800273 2209 5 5.498995856083596 2209 6 5.717576942360324 2209 7 6.080315072877671 2209 8 4.822316107579155 2209 9 4.63522163480668 2209 10 3.624055771735243 2209 11 -0.12644640163630427 2209 12 3.4750840025584893 2209 13 2.676557769357597 2209 14 4.063407587911058 2209 15 4.282923111778601 2209 16 3.6336640269229186 2209 17 1.7676682573409082 2209 18 3.4906457392615913 2209 19 4.171851825852595 2209 20 4.207145299288328 2209 21 6.544705180340195 2209 22 5.207739847244042 2209 23 0.8711079718412336 2209 24 -2.9102330761677884 2209 25 0.7006997050718518 2210 1 -2.192290951387541 2210 2 -13.905968522068605 2210 3 -7.797201817182751 2210 4 -2.1851914175845977 2210 5 -0.6559522449671285 2210 6 -1.569977222300242 2210 7 -1.2781151432145457 2210 8 -20.85958687749547 2210 9 -13.043263312373924 2210 10 -7.354554497601189 2210 11 -9.940350080585013 2210 12 -4.567198064336637 2210 13 -5.074360905767723 2210 14 -3.9492963261051983 2210 15 -5.526367720587086 2210 16 -4.922166285462026 2210 17 -4.673425019160835 2210 18 -4.900575337517697 2210 19 -10.043856279729551 2210 20 -6.9664152660066385 2210 21 -4.98763607826243 2210 22 -9.800954819838006 2210 23 -7.709289377276555 2210 24 -10.633035690232191 2210 25 -7.99399746845331 2211 1 0.41532718666024593 2211 2 5.402451534695821 2211 3 1.2450743968704856 2211 4 2.0769796332753927 2211 5 1.6176372164529873 2211 6 0.3710915359016056 2211 7 -20.653826718167107 2211 8 -12.733759062925934 2211 9 -8.400826247823687 2211 10 -6.60178404526302 2211 11 -9.54733451075956 2211 12 -4.194708884943452 2211 13 -6.553391996948054 2211 14 -4.271967629191696 2211 15 -4.8231889485441926 2211 16 -11.65224353319513 2211 17 -7.356986672833198 2211 18 -5.417385650619654 2211 19 -8.255739110195641 2211 20 -6.373218021289485 2211 21 -4.177375934897242 2211 22 -5.870756036628929 2211 23 -8.189077147971247 2211 24 -22.03056889706331 2211 25 -21.843143578510237 2212 1 -1.391698907112925 2212 2 -13.905968522068605 2212 3 -7.968182429645944 2212 4 -19.48025783124684 2212 5 -20.261265337946256 2212 6 -20.66675141584863 2212 7 -10.113955040806887 2212 8 -9.631355896743866 2212 9 -5.505203074825726 2212 10 -5.641416819479172 2212 11 -6.103977969279299 2212 12 -3.1798838825804374 2212 13 -6.31830436588735 2212 14 -3.964374845020526 2212 15 -4.2469242143770565 2212 16 -4.110553417021428 2212 17 -4.362827009222698 2212 18 -3.572416014298746 2212 19 -4.033323318134162 2212 20 -4.226008592116244 2212 21 -2.1020681140494517 2212 22 -3.387223010328775 2212 23 -3.4361515600136983 2212 24 -2.554858539593102 2212 25 -2.8898896830015275 2213 1 -18.774833645884694 2213 2 -13.905968522068605 2213 3 -8.869668089157363 2213 4 -6.023492826160368 2213 5 -20.261265337946256 2213 6 -10.8000169295821 2213 7 -20.653826718167107 2213 8 -10.842292879530058 2213 9 -8.723824310441646 2213 10 -7.6448780434209205 2213 11 -6.891058615237675 2213 12 -5.349902522091913 2213 13 -19.18631536497076 2213 14 -12.570010709715762 2213 15 -7.479049072063127 2213 16 -6.683744037029953 2213 17 -5.318643254783112 2213 18 -5.411574466930237 2213 19 -6.214703760913473 2213 20 -6.248773226090879 2213 21 -4.565321049143112 2213 22 -5.28675613672219 2213 23 -5.105141250004774 2213 24 -4.053966659016517 2213 25 -4.921475216353574 2214 1 -18.774833645884694 2214 2 -13.905968522068605 2214 3 -12.183522327791687 2214 4 -19.48025783124684 2214 5 -20.261265337946256 2214 6 -20.66675141584863 2214 7 -16.36077858982486 2214 8 -14.757982599420604 2214 9 -13.6174741110629 2214 10 -13.500267797583938 2214 11 -14.46465575593433 2214 12 -11.93504350163179 2214 13 -19.18631536497076 2214 14 -16.543034466522656 2214 15 -23.77304703747513 2214 16 -18.120631008594682 2214 17 -23.514980996390435 2214 18 -17.60968349691049 2214 19 -14.649824884180129 2214 20 -13.393912247203811 2214 21 -10.90847444293381 2214 22 -11.955034516232693 2214 23 -11.927419444552733 2214 24 -10.724686544122134 2214 25 -10.47629865872414 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 118621.0 3 0.0 4 523.0 5 2148.0 6 2836.0 7 2805.0 8 2403.0 9 2243.0 10 2062.0 11 1923.0 12 1794.0 13 2024.0 14 2929.0 15 6455.0 16 9657.0 17 10189.0 18 10453.0 19 12221.0 20 14584.0 21 16539.0 22 17040.0 23 18399.0 24 19439.0 25 20013.0 26 16413.0 27 12842.0 28 13480.0 29 15857.0 30 15424.0 31 17542.0 32 22886.0 33 28857.0 34 35070.0 35 40702.0 36 40498.0 37 42221.0 38 32301.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.37522413945148 19.825869367118376 13.178113701166993 25.620792792263146 2 12.692561925089437 20.817375859685242 43.71515215734952 22.774910057875804 3 17.86766129779155 25.710821665221307 30.31834370342929 26.103173333557855 4 11.512032564130005 17.76671476493968 39.482900510382954 31.23835216054736 5 11.500700745002582 38.34458213469056 37.8086966142952 12.346020506011653 6 29.25276968749122 38.519024920163695 19.921896217491874 12.306309174853205 7 25.945922273121425 31.16835568324151 24.01685221031979 18.868869833317277 8 22.734084265690353 39.569845483652855 21.952297959682262 15.743772290974531 9 26.308445594540427 15.202672704670572 21.377197655833776 37.111684044955226 10 14.357613337515382 29.683049034509935 35.89451588776027 20.064821740214413 11 32.20108352433383 21.811947733507576 27.09551821058499 18.891450531573604 12 24.15904820485166 24.472662009925767 33.17329648385167 18.1949933013709 13 28.338885610401476 22.518893605680457 25.767070872437227 23.37514991148084 14 18.97912864467472 24.11248012654578 28.503083909066163 28.40530731971334 15 21.13118103800572 34.51295463833265 23.6024519820188 20.753412341642825 16 19.624645336750305 28.283822125756725 31.372661183823435 20.718871353669538 17 19.78857715267122 29.591191422814646 30.48048121657197 20.139750207942164 18 19.7193027635158 27.282194073811784 33.85266614089585 19.14583702177657 19 22.67187200905948 26.618988561437035 27.962790488185835 22.746348941317645 20 22.822407847734137 28.457884027999487 29.566554370114822 19.153153754151557 21 21.044860402929793 26.660798219535288 27.405371290006475 24.888970087528442 22 21.99417741953674 30.321240818641282 28.218409600974404 19.466172160847577 23 20.204768632375767 29.409050475452027 29.873799477692526 20.512381414479684 24 22.27104306487696 27.462772651006713 30.393246644295303 19.87293763982103 25 21.223927362909542 28.299732653391306 30.042876941275715 20.433463042423437 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 654.0 1 654.0 2 1476.0 3 2298.0 4 2298.0 5 2298.0 6 3242.5 7 4187.0 8 4187.0 9 4187.0 10 4941.0 11 5695.0 12 5695.0 13 5695.0 14 7168.0 15 8641.0 16 8641.0 17 8641.0 18 12828.5 19 17016.0 20 17016.0 21 17016.0 22 23978.5 23 30941.0 24 30941.0 25 30941.0 26 40208.5 27 49476.0 28 49476.0 29 49476.0 30 59224.5 31 68973.0 32 68973.0 33 68973.0 34 73611.5 35 78250.0 36 78250.0 37 78250.0 38 78966.0 39 79682.0 40 79682.0 41 79682.0 42 78033.5 43 76385.0 44 76385.0 45 76385.0 46 73556.0 47 70727.0 48 70727.0 49 70727.0 50 62900.0 51 55073.0 52 55073.0 53 55073.0 54 45697.5 55 36322.0 56 36322.0 57 36322.0 58 29705.5 59 23089.0 60 23089.0 61 23089.0 62 18075.5 63 13062.0 64 13062.0 65 13062.0 66 9808.5 67 6555.0 68 6555.0 69 6555.0 70 4689.5 71 2824.0 72 2824.0 73 2824.0 74 1929.0 75 1034.0 76 1034.0 77 1034.0 78 693.5 79 353.0 80 353.0 81 353.0 82 235.0 83 117.0 84 117.0 85 117.0 86 71.5 87 26.0 88 26.0 89 26.0 90 18.5 91 11.0 92 11.0 93 11.0 94 6.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.793152600678182 2 37.22356757201933 3 5.945267052374669 4 14.16661255351263 5 14.113713645859235 6 15.440145836269961 7 9.589590001789695 8 7.944338945791291 9 0.1141919533475981 10 1.534385081874522 11 5.953502810452444 12 6.016538035740022 13 16.801421618548193 14 1.677243808531295 15 3.1110576138791526 16 3.0955363775018094 17 3.0795400012353635 18 1.596945167272998 19 1.54008676054375 20 0.2866677330917511 21 4.64100805678872 22 1.6956158842432525 23 0.11561737301490514 24 9.38132035039983 25 0.11894335223862158 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 631393.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.74369778126446 #Duplication Level Percentage of deduplicated Percentage of total 1 96.59546143339601 86.68833897920479 2 2.3106645392036778 4.147351601603591 3 0.45420902842447386 1.2228719332934321 4 0.19506247394608295 0.7002251084113218 5 0.11285881161913539 0.5064183540950172 6 0.06730767176451519 0.3624263611917119 7 0.04419835649379096 0.27765667633251545 8 0.0358079182404813 0.2570827994199985 9 0.025475201724247568 0.20576149240118588 >10 0.13440380979724995 2.2825668587798815 >50 0.013844462695620695 0.8756794004486675 >100 0.009598745174619821 1.6322382899092043 >500 7.383650133270956E-4 0.47063726593535254 >1k 3.691825066635478E-4 0.3707448789733611 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1202 0.1903727155670082 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1036 0.16408164170334483 No Hit CTGTAGGACGTGGAATATGGCAAGA 858 0.13589000828327205 No Hit CTTTAGGACGTGAAATATGGCGAGG 775 0.12274447135144038 No Hit GTCCTACAGTGTGCATTTCTCATTT 691 0.10944055445657459 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTACA 40 4.3538076E-4 20.680563 2 GAGTAAC 45 0.0011487645 17.903698 1 TAAACTA 30 0.009769165 17.472633 5 GTCTAAG 55 2.2308281E-4 17.089893 1 GTCCTAC 850 0.0 15.00751 1 GATATAC 175 0.0 14.578725 1 TCCTACA 980 0.0 13.927728 2 TGTTAGA 60 0.0065572374 13.787044 2 TACTATG 60 0.0065572374 13.787044 2 ACTGACC 50 0.0012009139 13.694305 8 AACTGCC 45 0.007038529 13.64174 7 TTACTAA 60 0.0078096017 13.427773 1 AAATGTC 185 0.0 13.273044 7 CTACATC 50 0.0086456975 13.2236395 3 TTTAGGA 515 0.0 13.117769 2 TAGGACC 305 0.0 13.0704155 4 TGTAGGA 700 0.0 12.999213 2 GCAGGAC 60 0.0019319127 12.856317 3 TCCTATA 110 1.6391785E-5 12.533676 2 AATGTCC 180 0.0 12.498771 8 >>END_MODULE