FastQCFastQC Report
Thu 26 May 2016
SRR1926701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926701_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631393
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA25620.40576946529340674No Hit
CTTTAGGACGTGAAATATGGCGAGG25490.40371052577396327No Hit
GTCCTACAGTGGACATTTCTAAATT24210.38343789050559635No Hit
GTCCTAAAGTGTGTATTTCTCATTT22580.35762195653103535No Hit
GTCCTACAGTGTGCATTTCTCATTT15830.25071548148300665No Hit
CTGTAGGACCTGGAATATGGCGAGA13910.22030652858045624No Hit
CTGAAGGACCTGGAATATGGCGAGA12160.1925900350494858No Hit
GTATCAACGCAGAGTACTTTTTTTT11300.17896935822855178No Hit
CCCATGTACTCTGCGTTGATACCAC9500.1504609648824108No Hit
GTACATGGGAAGCAGTGGTATCAAC9070.14365062647194377No Hit
GAATATGGCAAGAAAACTGAAAATC7680.12163581161020157No Hit
GTCCTTCAGTGTGCATTTCTCATTT7020.11118273404994987No Hit
GGTATCAACGCAGAGTACTTTTTTT6770.10722323497409694No Hit
GGAATATGGCGAGAAAACTGAAAAT6630.10500591549161932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAG250.00614235518.93838118
TATAGCG250.00614235518.93838116
AATACGG350.002204139716.24576614
TAGGACC3950.015.7066114
TCCAACG707.4934305E-613.52741518
AGGACCT7450.013.32426555
GGACCTG7500.012.852626
GTCTTAC550.002958834612.1479111
ATACTCC550.00296060512.1469416
ATAGGAC1353.4015102E-1012.02323
ACTGTTC2300.011.9764088
GTCCTAA7250.011.8487011
GCCATAT4500.011.665851
GATATAC2400.011.5332841
TAGAGCT751.9815569E-411.4537454
GTCGTCA751.9815569E-411.4537451
GGCGAGG6750.011.36302819
GTAGGAC12600.011.1391413
CTAGTAC600.005653622611.1391413
TGTAGGA12950.011.1365562
Produced by FastQC (version 0.11.5)