FastQCFastQC Report
Thu 26 May 2016
SRR1926700_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926700_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623372
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12560.20148482767913864No Hit
GTCCTAAAGTGTGTATTTCTCATTT9680.15528448502659728No Hit
CTGTAGGACGTGGAATATGGCAAGA9190.14742401006140796No Hit
CTTTAGGACGTGAAATATGGCGAGG7870.12624885301232652No Hit
GTCCTACAGTGTGCATTTCTCATTT6740.10812163523546132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCATA350.00592075919.056441
GTCTTAG450.001200726817.786011
GTCTAGG552.346073E-416.9775561
CTATACA400.002321342216.1186354
GTCCTAT851.6002796E-615.6935381
TATAGGA706.759641E-515.6523972
TGTAGGA6450.015.2883882
TAGGACC2800.014.9673034
TCTTACA656.842517E-414.7493752
CTGTAGG6650.014.0415881
CCAATAC806.5352815E-613.7053993
CTATAGG600.00815763913.3395091
ATTAGAC500.00893824513.1571833
GTAGGAC7150.013.0345053
GTAAGAC851.2213537E-512.8991993
GTTGGAC600.002006496312.7917063
GTCCTAA3550.012.7758671
TGTGCTA500.002158599712.66875712
GATATAC1902.910383E-1112.6374291
GTATCAA3800.012.6374291
Produced by FastQC (version 0.11.5)