##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926700_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 623372 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.71149329774196 27.0 10.0 31.0 2.0 31.0 2 16.6879295188106 22.0 2.0 31.0 2.0 31.0 3 20.405282880848034 25.0 15.0 31.0 2.0 31.0 4 22.445352053027726 28.0 7.0 35.0 2.0 37.0 5 23.29079586506933 30.0 7.0 35.0 2.0 37.0 6 23.726564555353786 31.0 2.0 35.0 2.0 37.0 7 23.688702732878603 31.0 8.0 35.0 2.0 37.0 8 23.830088614823893 31.0 11.0 35.0 2.0 37.0 9 26.726628722496358 32.0 17.0 37.0 2.0 39.0 10 27.498769595041164 32.0 25.0 38.0 2.0 39.0 11 24.55148931937912 30.0 8.0 37.0 2.0 39.0 12 25.751017049209782 31.0 17.0 37.0 2.0 39.0 13 22.27940940561976 28.0 2.0 37.0 2.0 39.0 14 25.747818317152518 31.0 17.0 38.0 2.0 40.0 15 26.900427032333823 31.0 17.0 38.0 2.0 40.0 16 27.282892077282906 32.0 20.0 39.0 2.0 40.0 17 26.614392369243404 31.0 17.0 39.0 2.0 40.0 18 26.707896729400744 31.0 17.0 38.0 2.0 40.0 19 27.106231912886688 31.0 19.0 39.0 2.0 40.0 20 27.38107261795525 31.0 23.0 39.0 2.0 40.0 21 25.217669385214606 30.0 10.0 38.0 2.0 40.0 22 26.175152236545756 31.0 15.0 38.0 2.0 40.0 23 26.17947710195517 31.0 9.0 39.0 2.0 40.0 24 25.156948018197802 31.0 2.0 39.0 2.0 40.0 25 24.982997311396726 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.960326639337691 1101 2 10.705176551082495 1101 3 7.331302788235771 1101 4 8.720353458200577 1101 5 8.519355913059414 1101 6 7.960203525906078 1101 7 7.694691933506643 1101 8 5.9726184504907955 1101 9 5.757771194442302 1101 10 5.583107257460309 1101 11 8.335652349485684 1101 12 5.812410140051405 1101 13 10.280419690185738 1101 14 7.5512715849675835 1101 15 6.7632905692033525 1101 16 6.453553873038036 1101 17 2.659637981003012 1101 18 4.741905542090457 1101 19 5.514709871716953 1101 20 3.618144036434977 1101 21 1.140592059868883 1101 22 4.799965056262579 1101 23 6.068911873099456 1101 24 7.1247211216647734 1101 25 7.843999604294208 1102 1 5.633765438397351 1102 2 10.687322095934995 1102 3 6.696242691048191 1102 4 7.710301424141921 1102 5 7.822872691534801 1102 6 6.487074350997101 1102 7 6.798003012604145 1102 8 6.894746543148688 1102 9 5.853339877966281 1102 10 5.09056654761827 1102 11 8.053417751853736 1102 12 5.456651453373844 1102 13 9.534063328556197 1102 14 7.003720625992788 1102 15 4.815186658765004 1102 16 5.333194651685435 1102 17 6.16978498080233 1102 18 6.168535548397617 1102 19 5.195706510288094 1102 20 5.142677450041237 1102 21 4.313766567480478 1102 22 5.386040749333304 1102 23 5.5903463574892385 1102 24 7.177868788016081 1102 25 6.76794548457287 1103 1 5.097460857815982 1103 2 9.662744647379427 1103 3 6.410503485233974 1103 4 7.771741040514783 1103 5 7.273561598797318 1103 6 6.694274301868905 1103 7 6.893561805794906 1103 8 6.473865635416249 1103 9 5.415450112028321 1103 10 4.675949485003851 1103 11 5.79731388487723 1103 12 4.454688105500821 1103 13 8.869045541834872 1103 14 7.078713216713073 1103 15 6.079357518090152 1103 16 5.8001786342912105 1103 17 3.583981961732224 1103 18 2.760377241682807 1103 19 4.639539720059265 1103 20 4.945540769523152 1103 21 7.22179448867244 1103 22 3.137099631079419 1103 23 5.271257827944236 1103 24 6.921748010971765 1103 25 2.7567705997615803 1104 1 6.387168639673458 1104 2 11.045390498031207 1104 3 7.721902867980589 1104 4 7.8885205865477595 1104 5 8.220247271239554 1104 6 7.626285222372122 1104 7 7.768002077886731 1104 8 7.534588567170051 1104 9 6.539335976613042 1104 10 5.487429216664541 1104 11 8.587089854732056 1104 12 6.088497712451307 1104 13 10.164755604732871 1104 14 7.8591600261662755 1104 15 6.819673302352012 1104 16 6.576423847016041 1104 17 6.841618800276077 1104 18 4.52546394467587 1104 19 5.207196545938391 1104 20 4.757256770423059 1104 21 4.0143014529688585 1104 22 6.02066145935164 1104 23 6.359049223053287 1104 24 7.523411103197553 1104 25 6.365229038525719 1105 1 4.002768099392785 1105 2 10.001061537308392 1105 3 6.708225423681135 1105 4 6.226666589513709 1105 5 2.7277753345470153 1105 6 5.078459953821596 1105 7 6.315990142873034 1105 8 3.499477954623025 1105 9 4.470839376260482 1105 10 3.9538028497468076 1105 11 7.443434718631693 1105 12 4.6147804338989715 1105 13 5.594784933528253 1105 14 4.305059463755466 1105 15 4.869064214749724 1105 16 5.119990971293316 1105 17 5.433226135272985 1105 18 2.946020555864564 1105 19 2.8341256201099725 1105 20 2.0600632283541707 1105 21 2.752621444625543 1105 22 4.46746390842052 1105 23 1.9120305599681444 1105 24 5.036423371435205 1105 25 5.734868520731304 1106 1 6.474538167057283 1106 2 11.43946907307663 1106 3 8.036835360502216 1106 4 9.194892628707489 1106 5 8.724609194723985 1106 6 7.43593753651912 1106 7 7.294502539567247 1106 8 6.811321145645103 1106 9 5.998754471917511 1106 10 5.227484788531388 1106 11 7.708613528092027 1106 12 4.984694911731339 1106 13 4.662851438544905 1106 14 5.525391819571691 1106 15 5.701328203611954 1106 16 5.643995152188996 1106 17 6.203910778098393 1106 18 6.0962738216603505 1106 19 5.695898776897792 1106 20 5.562404614566521 1106 21 5.6072447620054575 1106 22 6.156627009900337 1106 23 6.380922241763049 1106 24 7.13498417850041 1106 25 6.453873399853485 1107 1 4.206237335569622 1107 2 -14.166982539826595 1107 3 -5.8920531220847465 1107 4 2.191836776505358 1107 5 4.781135396350393 1107 6 4.883405051987449 1107 7 5.053204072479783 1107 8 5.499704003951461 1107 9 3.6656640886686063 1107 10 3.1930052682262158 1107 11 6.064178594806339 1107 12 3.469797528361923 1107 13 5.38805744054779 1107 14 4.450863764654446 1107 15 4.061100157420963 1107 16 3.7379146129893996 1107 17 4.402600177357904 1107 18 -0.5815934810535168 1107 19 1.910663604263334 1107 20 2.109475293095361 1107 21 -0.8166585344421264 1107 22 2.424443326339361 1107 23 -0.5043241503450879 1107 24 2.965465410814158 1107 25 3.797446095809903 1108 1 5.378830939091991 1108 2 10.901485928641872 1108 3 0.4790863672693497 1108 4 6.248506611353729 1108 5 7.706861031814533 1108 6 7.5617647462173 1108 7 7.691799836864547 1108 8 7.260210588082931 1108 9 5.745040850461958 1108 10 4.758567945420996 1108 11 7.172241211074546 1108 12 2.5412440876353557 1108 13 3.7294931955092423 1108 14 5.165583624279627 1108 15 5.246286064698847 1108 16 4.950073119557285 1108 17 0.14807983194486596 1108 18 3.460092479027395 1108 19 4.690319003576082 1108 20 5.080592848883793 1108 21 5.31667723595406 1108 22 1.8062969563444788 1108 23 4.858495206432789 1108 24 5.772975626169277 1108 25 6.309750013794616 1109 1 3.6283855928959348 1109 2 -14.166982539826595 1109 3 -5.858286983418449 1109 4 2.1406792372374035 1109 5 1.6302640382203712 1109 6 2.856999924738595 1109 7 3.9256640126550693 1109 8 4.02556290924262 1109 9 3.413152021122702 1109 10 2.4349990507472867 1109 11 0.7662794419399752 1109 12 1.4364792661566526 1109 13 2.7056359104621563 1109 14 3.4565746152706183 1109 15 3.327561107446975 1109 16 3.2348011904043332 1109 17 3.7037136527344927 1109 18 3.751200867869496 1109 19 2.50945995756123 1109 20 1.4210670341171827 1109 21 0.7573402786001076 1109 22 2.952416446939889 1109 23 3.2572676332505424 1109 24 4.428338921475916 1109 25 4.801348411058736 1110 1 3.4693965046575563 1110 2 9.426535978691923 1110 3 6.5987059868889695 1110 4 7.765723828570948 1110 5 8.521775946729448 1110 6 4.21729527674783 1110 7 6.759317354382063 1110 8 7.326827204699548 1110 9 5.740536345957452 1110 10 5.1379473248003755 1110 11 3.331275245108582 1110 12 6.027330173721442 1110 13 10.529893595909641 1110 14 7.9334264671224695 1110 15 7.0887535321663115 1110 16 6.657930977415145 1110 17 7.137794546659581 1110 18 7.054637023571939 1110 19 6.2003790636361416 1110 20 6.316628884919826 1110 21 4.031416975693801 1110 22 6.178268928316449 1110 23 6.71775446569205 1110 24 7.713841492035144 1110 25 7.936276540321142 1111 1 5.157209317470368 1111 2 9.712296739452686 1111 3 7.286293574476559 1111 4 7.90706516991229 1111 5 8.083437608391108 1111 6 7.356359340811892 1111 7 7.132340377405086 1111 8 7.251201579073921 1111 9 5.612608418029524 1111 10 5.0648742517273 1111 11 6.873142111975447 1111 12 6.201604447995713 1111 13 7.957421123437172 1111 14 5.576394435090435 1111 15 5.336376154788937 1111 16 5.1149379844221485 1111 17 6.228260012125045 1111 18 6.092524911459829 1111 19 4.98581449907158 1111 20 4.776088344379286 1111 21 7.585145704422526 1111 22 6.805396055443577 1111 23 6.831468179405764 1111 24 6.587390040583692 1111 25 6.946686950731554 1112 1 -1.6188846924444817 1112 2 7.95550374902167 1112 3 6.163273662425567 1112 4 -0.48874160578479575 1112 5 4.089790030282835 1112 6 3.246577895747084 1112 7 5.633990656569075 1112 8 5.897844199756761 1112 9 4.772955602508336 1112 10 3.237229605252672 1112 11 -0.22671954126828808 1112 12 4.095405195726993 1112 13 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1.1356428942456844 2204 6 3.87877939119403 2204 7 4.605403013500897 2204 8 4.999453148178571 2204 9 3.3274241816604544 2204 10 2.9163921203067815 2204 11 4.320791657255324 2204 12 4.729032824239258 2204 13 2.409233599420265 2204 14 3.9500975220636647 2204 15 4.349210790372386 2204 16 4.201629415189409 2204 17 -1.7377320462840764 2204 18 2.2338980381410103 2204 19 3.3385833816982817 2204 20 3.8063603225185645 2204 21 5.350027647977459 2204 22 4.940740404532001 2204 23 3.5881950484549066 2204 24 5.280951374429385 2204 25 5.260482262631129 2205 1 5.5373894976505476 2205 2 10.116801243957187 2205 3 7.015122403305387 2205 4 7.401659764506885 2205 5 7.271725896679396 2205 6 6.873476457929009 2205 7 6.968376413441124 2205 8 6.670120497992841 2205 9 5.330626436047542 2205 10 4.728838215691265 2205 11 6.980349319182654 2205 12 6.034036880428147 2205 13 9.549313015329062 2205 14 6.806124164820165 2205 15 5.555295073707853 2205 16 5.198721768205932 2205 17 5.961593345458379 2205 18 5.720452839387757 2205 19 4.366895580152661 2205 20 4.7238360921270335 2205 21 -1.6202597009828779 2205 22 3.133323983371504 2205 23 4.462999710937293 2205 24 6.172975626169276 2205 25 6.560200464245067 2206 1 4.076013487494052 2206 2 8.5873083364155 2206 3 6.06555332202899 2206 4 1.9959857978573083 2206 5 5.966220391173891 2206 6 6.241166098789382 2206 7 6.483481762692815 2206 8 6.353972642820597 2206 9 4.996091901513008 2206 10 3.984740459398388 2206 11 3.326239111413912 2206 12 4.498152215275191 2206 13 7.979758094554629 2206 14 0.48367488627332733 2206 15 2.4349820777851043 2206 16 3.512086352302223 2206 17 4.515549740878189 2206 18 -0.8449564723630196 2206 19 1.0589608161203365 2206 20 2.5540272589035666 2206 21 3.699145069641407 2206 22 -1.7695178828849976 2206 23 -2.5282256607758846 2206 24 1.5944873818273742 2206 25 3.219022701116085 2207 1 1.8267653675074556 2207 2 -14.166982539826595 2207 3 -6.3407259519879275 2207 4 -4.171991592678239 2207 5 1.5623076903139186 2207 6 2.314099504657513 2207 7 3.493947497939125 2207 8 3.4233444897922247 2207 9 0.9255810576441625 2207 10 0.6049497573902265 2207 11 -1.1139769219520979 2207 12 -2.0099055819231957 2207 13 0.6638916087235671 2207 14 -5.0176776203137194 2207 15 -1.0939211175064862 2207 16 0.15985135292478603 2207 17 1.341075973322134 2207 18 1.0357981569532413 2207 19 0.4659921790642443 2207 20 0.4486671211208737 2207 21 -3.3384677332519637 2207 22 -3.973346516916017 2207 23 -0.8016651389264453 2207 24 1.1963465456549045 2207 25 1.6425841430209758 2208 1 5.031616118637732 2208 2 6.870869572849461 2208 3 6.625596962371496 2208 4 8.622642190594945 2208 5 3.5689851277414455 2208 6 6.365306492188623 2208 7 7.045030708581333 2208 8 7.103913798335437 2208 9 5.495700665206279 2208 10 4.912119207951132 2208 11 6.991675610262469 2208 12 6.6381543782076164 2208 13 9.035794743536144 2208 14 3.2608586528293024 2208 15 5.139464278123537 2208 16 5.664314649819939 2208 17 6.390298459022649 2208 18 1.5428151665036047 2208 19 4.0948821935265975 2208 20 4.9645748943095 2208 21 7.527595901098078 2208 22 6.630564181315929 2208 23 2.538542154789596 2208 24 4.09933966626571 2208 25 5.582435903910085 2209 1 2.584587794848847 2209 2 4.7020650792210255 2209 3 5.1213148594978435 2209 4 0.12557323842035828 2209 5 4.739521164474663 2209 6 5.419442203894757 2209 7 6.112451257515968 2209 8 4.6372871151594595 2209 9 4.144748800169907 2209 10 3.452009188862238 2209 11 -0.309577420744084 2209 12 3.1820717284629936 2209 13 2.2187417847578317 2209 14 3.5774537361497387 2209 15 4.21177958019236 2209 16 3.5305602000443628 2209 17 1.3383134721785055 2209 18 3.300628069562986 2209 19 3.9453729586300383 2209 20 3.7752369435278865 2209 21 5.952530121806948 2209 22 4.575928675976197 2209 23 0.444026341963923 2209 24 -3.069074665881015 2209 25 0.34032619437079603 2210 1 -2.4160232961877774 2210 2 -14.166982539826595 2210 3 -7.986214136329025 2210 4 -2.4278573735208937 2210 5 -0.6895214241423915 2210 6 -1.6740848364407945 2210 7 -1.3598494913805261 2210 8 -21.337987610115267 2210 9 -13.355724131154087 2210 10 -7.318689970134791 2210 11 -10.579160674369891 2210 12 -4.966301175229059 2210 13 -5.735275828834249 2210 14 -4.358061103620422 2210 15 -5.92799730958453 2210 16 -5.201346191436496 2210 17 -4.980553830731353 2210 18 -4.972653613293165 2210 19 -10.206659406168285 2210 20 -7.1753762730427795 2210 21 -5.299446624850653 2210 22 -10.24607378964329 2210 23 -8.136540254560117 2210 24 -10.769762276143094 2210 25 -8.244431246769622 2211 1 0.115316999419111 2211 2 5.010879333399515 2211 3 0.8097715462989008 2211 4 1.7131396455231886 2211 5 1.3290221711942145 2211 6 0.07931654588830384 2211 7 -21.10549746043275 2211 8 -13.551800287504102 2211 9 -9.403046120141568 2211 10 -7.288809043677848 2211 11 -9.766828227332638 2211 12 -4.96340531105378 2211 13 -6.6485289136386925 2211 14 -5.0189851450573535 2211 15 -5.4213317896739746 2211 16 -11.932652084359974 2211 17 -7.7554751894909515 2211 18 -6.004595955859717 2211 19 -8.79199453039308 2211 20 -6.769337687139462 2211 21 -4.411872032330308 2211 22 -5.838178739786848 2211 23 -8.038792715358444 2211 24 -22.698195545001894 2211 25 -22.54520494116034 2212 1 -1.2907886805276299 2212 2 -14.166982539826595 2212 3 -8.043886605703623 2212 4 -19.904646541799423 2212 5 -20.66340923845574 2212 6 -21.1373343528818 2212 7 -10.16938634932164 2212 8 -9.72595057307823 2212 9 -5.341871061449954 2212 10 -5.750015638162587 2212 11 -6.294650680817345 2212 12 -3.328225381834116 2212 13 -6.774736033719986 2212 14 -4.076758718062717 2212 15 -4.2075427641299825 2212 16 -4.160587541103379 2212 17 -4.3473906385256065 2212 18 -3.4944370505021354 2212 19 -4.13943575117867 2212 20 -4.240778522487581 2212 21 -2.156545987269162 2212 22 -3.4828421827946627 2212 23 -3.4174307194931366 2212 24 -2.449121470927821 2212 25 -2.848908644864043 2213 1 -19.214862754601704 2213 2 -14.166982539826595 2213 3 -8.469155422907923 2213 4 -5.13367879986394 2213 5 -20.66340923845574 2213 6 -10.495398869010833 2213 7 -21.10549746043275 2213 8 -10.534761803663654 2213 9 -7.922904514257599 2213 10 -6.635648852021607 2213 11 -5.832553906623797 2213 12 -4.3804356566250355 2213 13 -19.618254552238504 2213 14 -12.34019957827777 2213 15 -6.441682549076219 2213 16 -5.865067827841727 2213 17 -4.508531618214974 2213 18 -4.524156285866532 2213 19 -5.353563588693607 2213 20 -5.38767219034899 2213 21 -3.494598555966892 2213 22 -4.5341564002379116 2213 23 -4.235053180663986 2213 24 -3.3121740396255497 2213 25 -4.249506016429155 2214 1 -19.214862754601704 2214 2 -14.166982539826595 2214 3 -12.013327860965774 2214 4 -19.904646541799423 2214 5 -20.66340923845574 2214 6 -21.1373343528818 2214 7 -16.274268987413233 2214 8 -14.646828023433292 2214 9 -13.017175096828616 2214 10 -13.090976432481591 2214 11 -14.065536635658523 2214 12 -11.666542344031674 2214 13 -19.618254552238504 2214 14 -16.62111386540294 2214 15 -24.382542764129983 2214 16 -18.04168844683115 2214 17 -24.1149832311182 2214 18 -17.71890339399833 2214 19 -14.528329955371383 2214 20 -13.150956266247414 2214 21 -10.506261086984262 2214 22 -11.476925186579157 2214 23 -11.491256037348306 2214 24 -10.312432054760793 2214 25 -10.116961542767687 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 103972.0 3 6.0 4 426.0 5 1874.0 6 2682.0 7 2638.0 8 2172.0 9 1958.0 10 1811.0 11 1780.0 12 1743.0 13 1839.0 14 2841.0 15 6398.0 16 9573.0 17 9968.0 18 10346.0 19 12088.0 20 13933.0 21 16449.0 22 17034.0 23 18084.0 24 19355.0 25 19991.0 26 16714.0 27 12831.0 28 13539.0 29 15739.0 30 15345.0 31 17674.0 32 22990.0 33 29292.0 34 36102.0 35 42515.0 36 42197.0 37 44654.0 38 34819.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.08873699928393 19.166584944796107 12.822809098327138 25.921868957592825 2 12.937685159907383 20.61455394788728 43.23742934854046 23.21033154366488 3 18.50374505430286 25.081416070521524 29.754707012569586 26.66013186260603 4 11.679372418320833 17.302760176535244 39.076370277654306 31.941497127489615 5 11.739271811737598 38.11533208840826 37.52445720337802 12.620938896476119 6 30.27212104167414 37.96784075530246 19.448811912397296 12.311226290626102 7 26.76523560654044 30.90237110374331 23.274748682818434 19.05764460689782 8 23.33140710777232 39.20971374663636 21.44741926388014 16.011459881711186 9 26.381490302386943 15.289418785602212 20.722073492120337 37.607017419890504 10 14.533590438298877 29.360736166369694 35.5512704727965 20.554402922534926 11 32.632410664219805 21.872695451224278 26.653752697490273 18.84114118706564 12 24.31102814425569 24.22917684135652 32.79106136125013 18.66873365313766 13 28.66007933148571 22.072424934832117 25.636404379894774 23.631091353787397 14 19.59500973769343 23.58086359895371 27.94693568233118 28.877190981021684 15 21.52845130835207 33.91653049186504 23.338350734517793 21.2166674652651 16 20.317879350562674 27.913646245646962 30.651684056802637 21.11679034698773 17 20.171948167785732 29.275620855115914 30.130076063526673 20.422354913571684 18 20.108372667814002 27.16952930507004 33.17398730333438 19.54811072378158 19 23.01203956554712 26.41625194882127 27.681236309428876 22.890472176202735 20 23.072294390403222 28.147574902420107 29.116294634956287 19.66383607222038 21 21.281460308513996 26.53166663311795 26.927373739406995 25.259499318961065 22 22.229357708444034 29.85744602475543 27.924833991283116 19.98836227551742 23 20.607790172190622 29.083063265037374 29.42448954683129 20.88465701594072 24 22.693423094098772 27.201642240747837 29.74831566182973 20.35661900332367 25 21.57161280789918 28.03932244112635 29.56538777188359 20.823676979090884 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 609.0 1 609.0 2 1332.5 3 2056.0 4 2056.0 5 2056.0 6 2902.5 7 3749.0 8 3749.0 9 3749.0 10 4374.0 11 4999.0 12 4999.0 13 4999.0 14 6266.5 15 7534.0 16 7534.0 17 7534.0 18 11249.0 19 14964.0 20 14964.0 21 14964.0 22 21284.5 23 27605.0 24 27605.0 25 27605.0 26 36444.0 27 45283.0 28 45283.0 29 45283.0 30 54837.0 31 64391.0 32 64391.0 33 64391.0 34 69535.5 35 74680.0 36 74680.0 37 74680.0 38 76447.0 39 78214.0 40 78214.0 41 78214.0 42 77299.5 43 76385.0 44 76385.0 45 76385.0 46 74219.5 47 72054.0 48 72054.0 49 72054.0 50 64993.5 51 57933.0 52 57933.0 53 57933.0 54 48738.0 55 39543.0 56 39543.0 57 39543.0 58 32660.0 59 25777.0 60 25777.0 61 25777.0 62 20320.5 63 14864.0 64 14864.0 65 14864.0 66 11201.5 67 7539.0 68 7539.0 69 7539.0 70 5450.0 71 3361.0 72 3361.0 73 3361.0 74 2288.0 75 1215.0 76 1215.0 77 1215.0 78 820.0 79 425.0 80 425.0 81 425.0 82 281.0 83 137.0 84 137.0 85 137.0 86 91.0 87 45.0 88 45.0 89 45.0 90 25.0 91 5.0 92 5.0 93 5.0 94 3.5 95 2.0 96 2.0 97 2.0 98 2.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.30200907323396 2 36.538054323902905 3 5.571151736042043 4 13.746045699838941 5 13.665515935909859 6 15.010459244239394 7 9.328298351546108 8 7.83577061529873 9 0.11116957450767759 10 1.41761259729343 11 5.592487310947556 12 5.730286249623018 13 16.243751724491958 14 1.5684053823399189 15 2.9107820049665367 16 2.8952214728925907 17 2.880623447957239 18 1.4750421898962418 19 1.4273980865358085 20 0.29356467727135643 21 4.367215723516616 22 1.5801158858594868 23 0.1106883209383803 24 9.11638636319886 25 0.11630294591351553 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 623372.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.90807650369784 #Duplication Level Percentage of deduplicated Percentage of total 1 96.51205902018295 86.7721358591601 2 2.40325365608573 4.321438271382946 3 0.48803892829819573 1.3163592390665066 4 0.1845836765430474 0.6638225324786448 5 0.09325534006095652 0.41922041242894187 6 0.06110532402917746 0.32963172885591197 7 0.03965571066565626 0.24957580678354307 8 0.03269350925263696 0.23515284248483495 9 0.026738260688403265 0.21635870287938994 >10 0.1353288923942217 2.2603106989031856 >50 0.012668536039644141 0.8085240477596145 >100 0.00968764174555502 1.636302305815536 >500 7.452032111118619E-4 0.5607882198302877 >1k 1.8630080277796547E-4 0.2103793321705022 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1256 0.20148482767913864 No Hit GTCCTAAAGTGTGTATTTCTCATTT 968 0.15528448502659728 No Hit CTGTAGGACGTGGAATATGGCAAGA 919 0.14742401006140796 No Hit CTTTAGGACGTGAAATATGGCGAGG 787 0.12624885301232652 No Hit GTCCTACAGTGTGCATTTCTCATTT 674 0.10812163523546132 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCATA 35 0.005920759 19.05644 1 GTCTTAG 45 0.0012007268 17.78601 1 GTCTAGG 55 2.346073E-4 16.977556 1 CTATACA 40 0.0023213422 16.118635 4 GTCCTAT 85 1.6002796E-6 15.693538 1 TATAGGA 70 6.759641E-5 15.652397 2 TGTAGGA 645 0.0 15.288388 2 TAGGACC 280 0.0 14.967303 4 TCTTACA 65 6.842517E-4 14.749375 2 CTGTAGG 665 0.0 14.041588 1 CCAATAC 80 6.5352815E-6 13.705399 3 CTATAGG 60 0.008157639 13.339509 1 ATTAGAC 50 0.008938245 13.157183 3 GTAGGAC 715 0.0 13.034505 3 GTAAGAC 85 1.2213537E-5 12.899199 3 GTTGGAC 60 0.0020064963 12.791706 3 GTCCTAA 355 0.0 12.775867 1 TGTGCTA 50 0.0021585997 12.668757 12 GATATAC 190 2.910383E-11 12.637429 1 GTATCAA 380 0.0 12.637429 1 >>END_MODULE