FastQCFastQC Report
Thu 26 May 2016
SRR1926700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926700_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623372
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA25460.40842386247698004No Hit
GTCCTACAGTGGACATTTCTAAATT23310.3739340233440065No Hit
CTTTAGGACGTGAAATATGGCGAGG22460.36029850554724946No Hit
GTCCTAAAGTGTGTATTTCTCATTT20720.33238579852800576No Hit
GTCCTACAGTGTGCATTTCTCATTT14690.2356538310992473No Hit
CTGAAGGACCTGGAATATGGCGAGA11930.1913785027238952No Hit
CTGTAGGACCTGGAATATGGCGAGA11440.18351802775870588No Hit
GTATCAACGCAGAGTACTTTTTTTT9920.15913451358097572No Hit
CCCATGTACTCTGCGTTGATACCAC8430.13523225297254288No Hit
GTACATGGGAAGCAGTGGTATCAAC7860.12608843515589407No Hit
GTCCTTCAGTGTGCATTTCTCATTT7650.12271966017081293No Hit
GAATATGGCAAGAAAACTGAAAATC7150.11469876734919117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC350.002196037716.25467913
TAAGAAC508.416525E-515.2592383
TAGGACC4200.014.5302624
GTACTAG400.005145498614.30322551
CGCCACC500.001519470713.27465414
GTTATAT655.255083E-513.2029781
TGGCGAA604.1803878E-412.63238718
CTCTAGG701.0516126E-412.2599081
TTATGCT550.002980464412.1360694
GTCTTAT550.002980464412.1360691
GTCTTAA550.002980464412.1360691
GGACCTG8350.012.1049256
GATATAC2600.012.102731
TGTAGGA13800.011.88960552
AGGACCT8350.011.8765315
GGCGAGG5600.011.84286219
CTGTAGG13500.011.7251131
ATGTCCA2150.011.4895399
TGTCCAC2000.011.37827610
GTCCTAC12550.011.32101251
Produced by FastQC (version 0.11.5)