FastQCFastQC Report
Thu 26 May 2016
SRR1926699_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926699_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572216
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT6270.10957400701832873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGA405.012405E-420.2527392
TAGGACC2600.017.5793464
TGTAGGA4300.016.9557822
CTTATAC400.00223495616.2100853
TCTATAC400.00223495616.2100853
CCCTAAG500.002631386315.8171121
CTGTAGG4500.015.2312941
GTCCTAC4000.014.499021
GTAGGAC5050.013.9096433
CTAGGAC550.001161580713.7540113
AGGACCT3700.013.6753865
GTCCTAA1851.8189894E-1213.53716851
CTAAGAC652.7010884E-413.3005813
GGACCTG3500.013.270516
GTACATA700.001600111313.1809261
GTACAGA700.001600111313.1809261
GTCTTAG600.00882721413.1809261
TCTAAAC500.00983361412.9680673
CTACAAC500.00983361412.9680673
TCCTACA4800.012.939252
Produced by FastQC (version 0.11.5)