FastQCFastQC Report
Thu 26 May 2016
SRR1926699_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926699_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572216
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12010.20988577739874453No Hit
CTGTAGGACGTGGAATATGGCAAGA11990.20953625903504972No Hit
GTCCTACAGTGTGCATTTCTCATTT10730.1875166021222755No Hit
CTGTAGGACCTGGAATATGGCGAGA10170.1777300879388203No Hit
GTATCAACGCAGAGTACTTTTTTTT9030.15780754120821508No Hit
CTTTAGGACGTGAAATATGGCGAGG7830.13683643938652537No Hit
GTCCTAAAGTGTGTATTTCTCATTT7530.13159366393110294No Hit
CCCATGTACTCTGCGTTGATACCAC7340.12827323947600205No Hit
GTACATGGGAAGCAGTGGTATCAAC7200.12582661093013828No Hit
GGTATCAACGCAGAGTACTTTTTTT6780.11848672529254688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATGC250.00589847219.0713023
TTATACT402.6813822E-416.6859254
GTCATTA350.002121816216.3382131
GTTCTGG551.0919823E-515.5955661
TAGGACC3500.015.5281244
GTAGGAC7500.014.494193
TGTAGGA8400.014.4182642
ACAGCGC400.005280792214.247118
CTGTAGG8500.013.9034981
GTCCTAA2200.013.8627251
TAAAGCC706.974622E-613.6211614
GGACCTG4850.013.56386
GGCGAGG2450.013.53903519
AGGACCT5250.013.4395475
TGGCGAG5800.012.74536718
CCTACAC1051.8822902E-812.7142023
TCCAACG604.1681863E-412.63643318
GACGTGG3250.012.5644817
GACCTGG5150.012.3545537
GGACGTG6200.012.3019046
Produced by FastQC (version 0.11.5)