FastQCFastQC Report
Thu 26 May 2016
SRR1926698_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926698_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664830
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8430.12679933216010109No Hit
GTCCTAAAGTGTGTATTTCTCATTT7030.10574131732924207No Hit
GTACATGGGAAGCAGTGGTATCAAC6830.1027330294962622No Hit
CCCATGTACTCTGCGTTGATACCAC6750.10152971436307026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGA853.0140654E-919.31252
GTCCTAG1008.0035534E-1118.6835271
CCTAGTA450.001014164918.2395862
GTCCTAT808.464831E-716.681721
GTATTGA654.4782602E-516.425081
GTACTAC500.002423201516.0144521
CTGTTAT708.4374915E-515.2518591
CTGTAGG5700.014.7501541
GTGTAGG550.00457081314.5585921
TGTAGGA5950.014.4843752
GTCCATG751.5182018E-414.2350671
GTCTTAC751.5182018E-414.2350671
AGGACCT4250.013.7217455
TCCTATA903.757733E-513.6796892
TCATATA700.001209677413.6796882
TCCAACG701.4807665E-613.41270518
GACCTAA600.00813534913.3453771
GTCCTGA802.624463E-413.3453761
GTCCTAA3100.013.3453761
GTCTATG802.624463E-413.3453761
Produced by FastQC (version 0.11.5)