##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926698_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 664830 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.49297263962216 27.0 2.0 30.0 2.0 31.0 2 16.0321811590933 16.0 2.0 30.0 2.0 31.0 3 19.590150865634826 22.0 15.0 31.0 2.0 31.0 4 21.68314606741573 28.0 2.0 35.0 2.0 37.0 5 22.067445813215407 28.0 2.0 35.0 2.0 37.0 6 22.927903373794805 30.0 2.0 35.0 2.0 37.0 7 22.814764676684266 30.0 2.0 35.0 2.0 37.0 8 22.903943865349035 30.0 2.0 35.0 2.0 37.0 9 25.52092565016621 31.0 17.0 37.0 2.0 39.0 10 26.152706706977725 31.0 17.0 37.0 2.0 39.0 11 23.315909330204715 30.0 7.0 37.0 2.0 39.0 12 24.367439796639744 30.0 8.0 37.0 2.0 39.0 13 21.111839116766692 27.0 2.0 35.0 2.0 39.0 14 24.37030368665674 30.0 10.0 37.0 2.0 40.0 15 25.418388159379088 31.0 10.0 38.0 2.0 40.0 16 25.788285727178376 31.0 8.0 38.0 2.0 40.0 17 25.111022366620038 31.0 7.0 38.0 2.0 40.0 18 25.179560188318817 31.0 8.0 38.0 2.0 40.0 19 25.560195839537926 31.0 7.0 38.0 2.0 40.0 20 25.79076606049667 31.0 2.0 38.0 2.0 40.0 21 23.68423356346735 30.0 2.0 37.0 2.0 40.0 22 24.600502384068108 30.0 2.0 38.0 2.0 40.0 23 24.567754162718288 31.0 2.0 38.0 2.0 40.0 24 23.65912789735722 31.0 2.0 38.0 2.0 40.0 25 23.45215167787254 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.293997980984031 1101 2 10.138046721475725 1101 3 6.579159313157209 1101 4 8.046756854755255 1101 5 7.573944835542505 1101 6 7.35777638272986 1101 7 7.134911807966176 1101 8 5.484348111526344 1101 9 5.502602978757686 1101 10 5.235663497881877 1101 11 7.861149188807943 1101 12 5.602903631343306 1101 13 9.970486410026975 1101 14 7.3421111767230265 1101 15 6.643201583085439 1101 16 5.983053684666899 1101 17 2.160617719973132 1101 18 4.748152359452163 1101 19 5.210726496350308 1101 20 3.019349586248712 1101 21 -0.0026576520350154453 1101 22 4.1155540650114055 1101 23 5.237124462087223 1101 24 6.523059756886763 1101 25 7.107716554363737 1102 1 5.260497487756645 1102 2 10.393828867026325 1102 3 6.747395316540651 1102 4 8.244601019351844 1102 5 7.801751704354718 1102 6 6.9520019163973075 1102 7 7.003210544066217 1102 8 6.973875721180608 1102 9 5.909850632393621 1102 10 5.450768710810166 1102 11 7.9471564309600495 1102 12 5.059084332131171 1102 13 9.422619666237019 1102 14 7.228873656598509 1102 15 5.288140519930174 1102 16 5.662539169416583 1102 17 6.388437578507418 1102 18 6.11873231997869 1102 19 5.162827278255168 1102 20 5.2877768441871495 1102 21 4.4528521678128214 1102 22 5.488115340204782 1102 23 5.733758213816955 1102 24 7.357188647021985 1102 25 6.906675389934488 1103 1 4.722260037299183 1103 2 9.093601284951472 1103 3 6.162527304015455 1103 4 7.827751842924702 1103 5 6.633748450733545 1103 6 6.216178659083088 1103 7 6.487510809046235 1103 8 5.873573654866263 1103 9 4.890972461651749 1103 10 4.0179147723780915 1103 11 5.298398704973231 1103 12 3.929197375209707 1103 13 7.612855997872639 1103 14 6.050099794956878 1103 15 5.165931828492045 1103 16 4.816813481781356 1103 17 2.2269259847550558 1103 18 1.2376944023585708 1103 19 3.239426059861298 1103 20 3.7420940862757313 1103 21 5.831668413791569 1103 22 1.5526688671815556 1103 23 3.5951738974241145 1103 24 5.048170210614462 1103 25 0.7361158309809142 1104 1 5.866591555298392 1104 2 10.407018098744274 1104 3 7.331157987832825 1104 4 7.904551685990157 1104 5 7.959261523941642 1104 6 7.328165002041118 1104 7 7.442921470008457 1104 8 7.345616575218866 1104 9 6.249669095343478 1104 10 4.916714565623057 1104 11 7.925689279870554 1104 12 4.925010897113598 1104 13 9.08825323844022 1104 14 6.736683759609242 1104 15 6.1956427450956575 1104 16 6.002302952532059 1104 17 6.209857796072402 1104 18 3.8679819911402404 1104 19 4.774085552377329 1104 20 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6 3.5126128365268627 2204 7 4.78530862074895 2204 8 4.76691847143546 2204 9 3.190703439069594 2204 10 2.7996152173534803 2204 11 4.025664907680127 2204 12 4.422573678070794 2204 13 2.2357636643216807 2204 14 3.542022171986705 2204 15 3.820038705931651 2204 16 3.758857868192244 2204 17 -1.0874739004125864 2204 18 2.4482011001411017 2204 19 3.1826627580605624 2204 20 3.453728271128046 2204 21 5.17667251163985 2204 22 4.602826993191545 2204 23 3.632038325785853 2204 24 4.988471275128948 2204 25 5.1053991011101445 2205 1 5.958265179788356 2205 2 10.347090587953502 2205 3 7.195680317282562 2205 4 7.729100951804579 2205 5 7.91091620741723 2205 6 7.730492652086699 2205 7 7.800667666316436 2205 8 7.498675903078386 2205 9 6.309337716101297 2205 10 5.638574472924198 2205 11 7.744865286738886 2205 12 6.721951126707015 2205 13 9.839223891669189 2205 14 7.35321230241977 2205 15 6.422456229209693 2205 16 5.9992538032550335 2205 17 6.756493405769493 2205 18 6.598591053914067 2205 19 5.259044852798926 2205 20 5.70259014428083 2205 21 -0.4871636448915275 2205 22 3.978256761295917 2205 23 5.030739552315872 2205 24 6.808105407381262 2205 25 7.261009156111687 2206 1 4.117711515477584 2206 2 8.921971641136842 2206 3 6.209663048393633 2206 4 1.40283026571473 2206 5 5.747174250007678 2206 6 6.121407908959981 2206 7 6.452067811471871 2206 8 6.174553027848699 2206 9 4.90069779950807 2206 10 4.052188267298849 2206 11 3.5265064360012985 2206 12 4.91148841495 2206 13 8.176253072520193 2206 14 0.5749739537356717 2206 15 2.805626982693134 2206 16 3.664593478498542 2206 17 4.843133057396447 2206 18 -1.2111826015260938 2206 19 1.168788555529808 2206 20 2.5836937288137136 2206 21 3.3639015483322474 2206 22 -2.076414133310866 2206 23 -2.634281155374488 2206 24 1.2800189822947168 2206 25 2.9117418454617727 2207 1 1.2972117884081555 2207 2 -13.46493312076792 2207 3 -6.29840747921344 2207 4 -4.484924102641756 2207 5 0.747269628970308 2207 6 1.1565081008918021 2207 7 2.3841058984927024 2207 8 2.70821739955079 2207 9 0.1620845802773232 2207 10 -0.19213888547770708 2207 11 -2.23121412418136 2207 12 -2.739013718604024 2207 13 -0.5033785299050741 2207 14 -6.004437836864952 2207 15 -2.3065526173608646 2207 16 -1.1764721539666958 2207 17 -0.04393091400747551 2207 18 -0.19648470338495727 2207 19 -0.6312245040512288 2207 20 -0.7859496419081751 2207 21 -4.280130223133888 2207 22 -4.79472777455684 2207 23 -1.8587195933898997 2207 24 -0.2316964890534443 2207 25 0.38400417576058743 2208 1 5.0573066978128125 2208 2 6.622771797264868 2208 3 6.42415507471236 2208 4 8.245015500464369 2208 5 3.9107295081765194 2208 6 6.311814399425021 2208 7 6.790533853626439 2208 8 6.764343370215155 2208 9 5.132921508663863 2208 10 4.49021659338338 2208 11 6.425817799891117 2208 12 6.041141587694508 2208 13 8.465042522726506 2208 14 3.2366751916078726 2208 15 4.672826157445002 2208 16 4.9892310384405505 2208 17 5.855076851307452 2208 18 0.8565351413087434 2208 19 3.530768593446613 2208 20 4.508010668704422 2208 21 7.025909466253513 2208 22 6.167179041664042 2208 23 1.8411509846946572 2208 24 3.502557177900826 2208 25 4.6570412767096805 2209 1 2.7101328590149976 2209 2 4.218226601454301 2209 3 4.723782237736039 2209 4 0.5294246534698317 2209 5 4.771462090143732 2209 6 5.386182568823926 2209 7 5.840832692424254 2209 8 4.4849803237670685 2209 9 4.24366832105002 2209 10 3.4486239248952195 2209 11 -0.35164690866990256 2209 12 2.9500725986234713 2209 13 2.1758633332911685 2209 14 3.3728233018082356 2209 15 3.7282991765706868 2209 16 3.3945219082037568 2209 17 0.6887431764440635 2209 18 2.7208964801065605 2209 19 3.5444440260513517 2209 20 3.808520117702603 2209 21 5.848205686654243 2209 22 4.400219228367138 2209 23 0.13093638997698775 2209 24 -3.415134078929775 2209 25 -0.23784715680580248 2210 1 -2.0372911528575592 2210 2 -13.46493312076792 2210 3 -7.444726209563832 2210 4 -2.0427640676001992 2210 5 -0.6321602610735795 2210 6 -0.8286459178006602 2210 7 -0.7280333092288878 2210 8 -20.2676238428996 2210 9 -12.558355524256061 2210 10 -6.7281510099810475 2210 11 -9.574500017075874 2210 12 -4.549734848025164 2210 13 -5.288470463422588 2210 14 -3.857427455881375 2210 15 -5.331225639329524 2210 16 -4.933362791475634 2210 17 -4.422775740748602 2210 18 -4.620012183854968 2210 19 -9.949463930315748 2210 20 -6.662999989250611 2210 21 -4.762656514027054 2210 22 -9.37505144215686 2210 23 -7.389933732004778 2210 24 -10.028167592605758 2210 25 -7.52964219237338 2211 1 -0.5646978935283897 2211 2 3.8878496440435697 2211 3 -0.014063426038148918 2211 4 0.8589896594542736 2211 5 -0.097801262639031 2211 6 -1.1517344718702667 2211 7 -20.208646474242414 2211 8 -13.358288115790085 2211 9 -9.723616737796977 2211 10 -7.789709533113957 2211 11 -9.663021709188554 2211 12 -5.634597883123988 2211 13 -7.196646266748729 2211 14 -5.362578715446901 2211 15 -5.956755618462228 2211 16 -11.738368514197997 2211 17 -8.249518904645104 2211 18 -6.226487197332101 2211 19 -8.967252126452141 2211 20 -7.024175810384371 2211 21 -4.749618096509533 2211 22 -6.331910048014272 2211 23 -8.278472584190178 2211 24 -21.185967412263107 2211 25 -20.991319379028024 2212 1 -1.0014706560110653 2212 2 -13.46493312076792 2212 3 -7.576038720814509 2212 4 -19.1320336798635 2212 5 -19.376975409856268 2212 6 -20.212775764509406 2212 7 -9.639189252531283 2212 8 -8.888599003893159 2212 9 -4.989593459589866 2212 10 -5.46767699251253 2212 11 -5.43034369135415 2212 12 -2.766142711097473 2212 13 -5.849542664357815 2212 14 -3.3267472223152446 2212 15 -3.6187162136470405 2212 16 -3.6336240145704437 2212 17 -3.634032141709877 2212 18 -2.830961653901415 2212 19 -3.39510273235609 2212 20 -3.5280051365650067 2212 21 -1.448129473828228 2212 22 -2.88348001394095 2212 23 -2.897880339298286 2212 24 -1.840981211711128 2212 25 -2.198311099359209 2213 1 -17.943512974318335 2213 2 -13.46493312076792 2213 3 -8.58179457285271 2213 4 -5.650153170558896 2213 5 -19.376975409856268 2213 6 -10.561453531404606 2213 7 -20.208646474242414 2213 8 -10.58398035612193 2213 9 -8.051031863953789 2213 10 -7.206563323445188 2213 11 -6.67264722154399 2213 12 -5.140274895663175 2213 13 -18.43003944448661 2213 14 -11.955960604220712 2213 15 -6.970803520948088 2213 16 -6.277864062794176 2213 17 -4.880702118201707 2213 18 -4.912086910360305 2213 19 -5.648423294930257 2213 20 -5.58958410337695 2213 21 -3.962888080349785 2213 22 -4.63131728948029 2213 23 -4.299387060616112 2213 24 -3.436701986210217 2213 25 -4.308655324179835 2214 1 -17.943512974318335 2214 2 -13.46493312076792 2214 3 -11.917049071431874 2214 4 -19.1320336798635 2214 5 -19.376975409856268 2214 6 -20.212775764509406 2214 7 -15.95192001684331 2214 8 -14.383094694917537 2214 9 -13.221827443773098 2214 10 -13.234197138881562 2214 11 -13.488612840408816 2214 12 -11.677846870733779 2214 13 -18.43003944448661 2214 14 -15.904903871834016 2214 15 -22.89930499009598 2214 16 -17.439983105498285 2214 17 -22.62115265688927 2214 18 -16.974707966829165 2214 19 -14.200117975692546 2214 20 -12.834094014086139 2214 21 -10.415768840002459 2214 22 -11.460234028982141 2214 23 -11.413116456311005 2214 24 -10.295832883115125 2214 25 -10.01037767499215 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 130997.0 3 2.0 4 746.0 5 2527.0 6 3325.0 7 3316.0 8 2851.0 9 2611.0 10 2514.0 11 2311.0 12 2182.0 13 2339.0 14 3302.0 15 6825.0 16 9750.0 17 10414.0 18 10753.0 19 12682.0 20 15054.0 21 17368.0 22 18315.0 23 19894.0 24 20400.0 25 20911.0 26 17773.0 27 14386.0 28 15337.0 29 17486.0 30 16971.0 31 19522.0 32 24923.0 33 30423.0 34 36666.0 35 41044.0 36 40431.0 37 40700.0 38 27779.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.14649126907051 19.108158965756612 12.98776613884662 25.75758362632626 2 12.696202232869922 20.856090825406568 42.67307102225789 23.774635919465624 3 18.3861066467456 25.73099228908063 29.994695855918422 25.88820520825535 4 10.741135752898982 17.580190372624262 39.748040333064964 31.930633541411797 5 11.984210362079907 38.442602204447034 36.99539208853847 12.577795344934591 6 30.124537015021584 38.38649624944741 19.108004290371497 12.380962445159506 7 26.189148737758394 31.514557178366502 24.196808139940064 18.099485943935033 8 23.33655416528399 38.52326481745366 21.174367383057774 16.965813634204586 9 26.090760159247367 15.610940125639189 20.8361691491348 37.46213056597864 10 14.18071201044705 29.836795342763345 35.58114771104629 20.401344935743314 11 32.25173876058151 22.34136099632654 26.01179457933623 19.395105663755725 12 24.10985020861576 25.07093574506883 32.5750354079943 18.24417863832111 13 28.432603113981447 22.067500590040122 25.389247680963518 24.110648615014913 14 19.289302871392426 23.722908797535663 28.945567005268497 28.042221325803418 15 22.03776354127639 33.06879863263531 23.723127227463717 21.170310598624585 16 19.732284953193258 28.693362755747987 30.557574386383042 21.016777904675717 17 20.21611147910386 29.14084698861561 30.144487021549242 20.49855451073129 18 19.948314654764484 27.838496270900816 32.649995878605075 19.563193195729625 19 22.202879623088858 27.519059919167937 28.07655397271727 22.20150648502593 20 22.65996033511549 28.421624148826268 28.754628198264097 20.16378731779415 21 21.194580777417602 26.76252071195681 27.365722065190397 24.677176445435194 22 21.632426633412344 29.408069980790124 28.173564340850703 20.785939044946826 23 20.327592878965604 29.356724793974553 29.424483938824032 20.891198388235807 24 22.217539893441216 27.860957971416862 29.451572601435767 20.469929533706157 25 21.179372352616102 28.17622071408028 29.410825795551567 21.233581137752054 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 738.0 1 738.0 2 1546.0 3 2354.0 4 2354.0 5 2354.0 6 3363.0 7 4372.0 8 4372.0 9 4372.0 10 5143.0 11 5914.0 12 5914.0 13 5914.0 14 7450.0 15 8986.0 16 8986.0 17 8986.0 18 12897.5 19 16809.0 20 16809.0 21 16809.0 22 23495.5 23 30182.0 24 30182.0 25 30182.0 26 39268.5 27 48355.0 28 48355.0 29 48355.0 30 57768.5 31 67182.0 32 67182.0 33 67182.0 34 72821.0 35 78460.0 36 78460.0 37 78460.0 38 80654.5 39 82849.0 40 82849.0 41 82849.0 42 82675.5 43 82502.0 44 82502.0 45 82502.0 46 80987.0 47 79472.0 48 79472.0 49 79472.0 50 71282.0 51 63092.0 52 63092.0 53 63092.0 54 52053.0 55 41014.0 56 41014.0 57 41014.0 58 33432.5 59 25851.0 60 25851.0 61 25851.0 62 20159.0 63 14467.0 64 14467.0 65 14467.0 66 10826.0 67 7185.0 68 7185.0 69 7185.0 70 5165.0 71 3145.0 72 3145.0 73 3145.0 74 2187.0 75 1229.0 76 1229.0 77 1229.0 78 842.0 79 455.0 80 455.0 81 455.0 82 305.0 83 155.0 84 155.0 85 155.0 86 101.5 87 48.0 88 48.0 89 48.0 90 29.5 91 11.0 92 11.0 93 11.0 94 7.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.111772934434365 2 36.273934690071144 3 5.568190364454071 4 13.688912955191554 5 13.235864807544786 6 14.597265466359822 7 9.351563557601192 8 7.5330535625648665 9 0.10619256050418903 10 1.4039679316517004 11 5.577967299911255 12 5.799828527593521 13 15.875637380984612 14 1.5603988989666533 15 2.845238632432351 16 2.935637681813396 17 2.8100416647864868 18 1.4608245716950197 19 1.4134440383255868 20 0.26698554517696255 21 4.320502985725675 22 1.5761924100897975 23 0.10724546124573199 24 8.982446640494562 25 0.10995292029541387 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 664830.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.42938929968832 #Duplication Level Percentage of deduplicated Percentage of total 1 97.11864081843098 88.79498019644927 2 1.9781056544734208 3.6171398391753025 3 0.39423217798992055 1.0813322182771339 4 0.1625192683527885 0.5943614981971045 5 0.08062115290149977 0.36855713872104595 6 0.0494019497882619 0.2710074059612791 7 0.03814071953464869 0.24410278861525356 8 0.02662285117310659 0.19472888193389012 9 0.019788759872629697 0.16283468071374402 >10 0.11165907575776775 1.9487035576059548 >50 0.010389383065058428 0.6582271728134418 >100 0.008685993477474204 1.3541122679220976 >500 0.0011921951824541482 0.7099123536145295 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 843 0.12679933216010109 No Hit GTCCTAAAGTGTGTATTTCTCATTT 703 0.10574131732924207 No Hit GTACATGGGAAGCAGTGGTATCAAC 683 0.1027330294962622 No Hit CCCATGTACTCTGCGTTGATACCAC 675 0.10152971436307026 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.0082878329798594E-4 0.0 8 0.0 0.0 0.0 3.0082878329798594E-4 0.0 9 0.0 0.0 0.0 3.0082878329798594E-4 0.0 10 0.0 0.0 0.0 3.0082878329798594E-4 0.0 11 0.0 0.0 0.0 3.0082878329798594E-4 0.0 12 0.0 0.0 0.0 3.0082878329798594E-4 0.0 13 0.0 0.0 0.0 3.0082878329798594E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTAGA 85 3.0140654E-9 19.3125 2 GTCCTAG 100 8.0035534E-11 18.683527 1 CCTAGTA 45 0.0010141649 18.239586 2 GTCCTAT 80 8.464831E-7 16.68172 1 GTATTGA 65 4.4782602E-5 16.42508 1 GTACTAC 50 0.0024232015 16.014452 1 CTGTTAT 70 8.4374915E-5 15.251859 1 CTGTAGG 570 0.0 14.750154 1 GTGTAGG 55 0.004570813 14.558592 1 TGTAGGA 595 0.0 14.484375 2 GTCCATG 75 1.5182018E-4 14.235067 1 GTCTTAC 75 1.5182018E-4 14.235067 1 AGGACCT 425 0.0 13.721745 5 TCCTATA 90 3.757733E-5 13.679689 2 TCATATA 70 0.0012096774 13.679688 2 TCCAACG 70 1.4807665E-6 13.412705 18 GACCTAA 60 0.008135349 13.345377 1 GTCCTGA 80 2.624463E-4 13.345376 1 GTCCTAA 310 0.0 13.345376 1 GTCTATG 80 2.624463E-4 13.345376 1 >>END_MODULE