FastQCFastQC Report
Thu 26 May 2016
SRR1926698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926698_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664830
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC20740.31195944828001143No Hit
GTACATGGGAAGCAGTGGTATCAAC18480.27796579576733904No Hit
GTCCTACAGTGGACATTTCTAAATT17300.26021689755275784No Hit
CTGTAGGACGTGGAATATGGCAAGA16960.25510280823669207No Hit
CTTTAGGACGTGAAATATGGCGAGG16120.2424679993381767No Hit
GTCCTAAAGTGTGTATTTCTCATTT15800.2376547388054089No Hit
GTCCTACAGTGTGCATTTCTCATTT13270.1995998977182137No Hit
GAGTAAGCAGTGGTATCAACGCAGA12710.19117669178587007No Hit
GTATCAACGCAGAGTACTTTTTTTT12260.1844080441616654No Hit
CTGTAGGACCTGGAATATGGCGAGA10860.1633500293308064No Hit
GGTATCAACGCAGAGTACTTTTTTT8210.12349021554382324No Hit
CTGAAGGACCTGGAATATGGCGAGA8200.12333980115217424No Hit
GAGTACATGGGAAGCAGTGGTATCA7580.11401410886993668No Hit
TATCAACGCAGAGTACTTTTTTTTT7140.10739587563738098No Hit
GCTTACTCTGCGTTGATACCACTGC6660.10017598483822933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC350.002110497316.3518584
TAGGACC4300.014.8624444
TTATACT759.2174014E-713.9899234
GGACCTG6900.013.5465286
ATAGTAC500.001452889913.3540183
GCCGTGC500.001494026313.3046478
AGGACCT7400.012.7610785
TTCATAA751.4120013E-512.7210022
CCAACGA1001.4912439E-712.31529119
TGTAGGA11250.012.1273562
CTGCGCT550.00305897212.0942219
AACTGGC550.00305897212.0942217
TCCAACG1103.9504812E-812.05692818
TCCCGTT802.923827E-511.84964711
CTGTAGG10750.011.7940791
GGCGAGG5100.011.702311519
GTCCTAA4750.011.640061
GTAGGAC11050.011.4808293
GACCTGG7950.011.4748417
CTCTTAG752.0051273E-411.439371
Produced by FastQC (version 0.11.5)