FastQCFastQC Report
Thu 26 May 2016
SRR1926697_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926697_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631892
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8640.13673222639311783No Hit
GTCCTAAAGTGTGTATTTCTCATTT7220.11426003177758225No Hit
GTCCTACAGTGTGCATTTCTCATTT6370.10080836598659265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGG405.355457E-420.0555551
TAGGACC2200.017.5587734
TGTAGGA5800.016.5420282
CTGTAGG5500.015.8013481
AGGACGG558.945314E-515.1490445
CTACACT1155.4569682E-1114.9291974
TCTACAC1052.730303E-914.5858583
GTCTTAC550.004513758214.5858581
TTTAGGA4450.014.4762662
GTGTAAG658.2113215E-414.3988611
GTAGGAC6100.014.1674123
TCAGGAC550.001059512513.9228663
TTTAATC652.436603E-413.4638693
CCTTATA600.00803430313.370371
TAGGACA2250.013.3533384
GGACGGG550.00154139713.2482296
CTTTAGG4350.013.2166881
GCTCGGC450.00969304412.9971248
GATATAC1759.276846E-1112.988361
TTAGGAC4550.012.9830173
Produced by FastQC (version 0.11.5)