##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926697_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 631892 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.446441797015947 27.0 2.0 30.0 2.0 31.0 2 15.993630240610738 16.0 2.0 30.0 2.0 31.0 3 19.567144385432954 22.0 15.0 31.0 2.0 31.0 4 21.672755787381387 28.0 2.0 35.0 2.0 37.0 5 22.028835623809133 28.0 2.0 35.0 2.0 37.0 6 22.884822089850797 30.0 2.0 35.0 2.0 37.0 7 22.758854361188305 30.0 2.0 35.0 2.0 37.0 8 22.880506478955265 30.0 2.0 35.0 2.0 37.0 9 25.519349825603108 31.0 17.0 37.0 2.0 39.0 10 26.143961309844087 31.0 17.0 37.0 2.0 39.0 11 23.293670437353217 30.0 7.0 37.0 2.0 39.0 12 24.35374557677578 30.0 8.0 37.0 2.0 39.0 13 21.080334930652707 27.0 2.0 35.0 2.0 39.0 14 24.354538433782988 30.0 10.0 37.0 2.0 40.0 15 25.43517246618093 31.0 10.0 38.0 2.0 40.0 16 25.807508245079855 31.0 8.0 38.0 2.0 40.0 17 25.106038690155913 31.0 8.0 38.0 2.0 40.0 18 25.173322339893527 31.0 8.0 38.0 2.0 40.0 19 25.548386433124648 31.0 8.0 38.0 2.0 40.0 20 25.79047052344388 31.0 2.0 38.0 2.0 40.0 21 23.655026491868863 30.0 2.0 37.0 2.0 40.0 22 24.578244383533896 30.0 2.0 37.0 2.0 40.0 23 24.552912523026087 31.0 2.0 38.0 2.0 40.0 24 23.657042659188594 30.0 2.0 38.0 2.0 40.0 25 23.453406594797844 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.107485526000872 1101 2 9.71597947332707 1101 3 6.527880556348826 1101 4 7.731206459929883 1101 5 7.591357638718701 1101 6 7.176346357768644 1101 7 6.775500865237845 1101 8 5.197455595862678 1101 9 5.07484150115512 1101 10 4.6336484616026645 1101 11 7.247218599727841 1101 12 5.179562881083168 1101 13 9.631231936502516 1101 14 6.979283640744061 1101 15 6.305730168606932 1101 16 5.895428651818957 1101 17 1.5765555637469326 1101 18 4.055880752279869 1101 19 4.593578040639709 1101 20 2.4213841609609474 1101 21 0.1571409364645966 1101 22 4.033123557038881 1101 23 4.846473058668561 1101 24 6.311033956832244 1101 25 6.658853646919962 1102 1 5.252340738683767 1102 2 10.31305826614372 1102 3 6.584098093776262 1102 4 7.739084044205267 1102 5 7.806051090190341 1102 6 6.548944551746306 1102 7 6.795639541953058 1102 8 6.7309111412338964 1102 9 5.842133655821243 1102 10 4.8802664131931195 1102 11 7.926325433417624 1102 12 5.250231991990592 1102 13 9.603472830008304 1102 14 7.060994269796581 1102 15 4.830220951782408 1102 16 5.355498414523918 1102 17 6.309267652554347 1102 18 6.254743501371767 1102 19 5.007884487324706 1102 20 5.56543990322934 1102 21 4.580412994040046 1102 22 5.440144808354358 1102 23 5.858857211840981 1102 24 7.127712505224814 1102 25 7.074220113294679 1103 1 3.9174328178239044 1103 2 8.304250845044248 1103 3 5.536655086998689 1103 4 7.217139086308368 1103 5 6.216951685795188 1103 6 6.038273298237712 1103 7 6.040729224981742 1103 8 5.606142194838334 1103 9 4.5550196009160615 1103 10 3.6944051822764337 1103 11 4.414074457857961 1103 12 3.3027273709185003 1103 13 7.278197277191161 1103 14 5.736906503657057 1103 15 5.032166936584215 1103 16 4.686125853423075 1103 17 2.5293333744685 1103 18 1.2214786283656736 1103 19 3.3635375988465803 1103 20 3.8160423541090545 1103 21 5.70925019538187 1103 22 1.2773889378812946 1103 23 3.6112499002780325 1103 24 4.774327142042779 1103 25 1.0577245974461142 1104 1 5.571734308077335 1104 2 10.3916500447355 1104 3 7.095087104765273 1104 4 7.849462554583777 1104 5 7.603273312412561 1104 6 7.492829370631124 1104 7 7.22340714472066 1104 8 7.290434950757707 1104 9 6.19291713160472 1104 10 5.093076751003455 1104 11 8.300379157471351 1104 12 5.111770453529051 1104 13 9.324453700989174 1104 14 7.015227074029383 1104 15 6.213951110512564 1104 16 5.873294507320011 1104 17 6.071782915069612 1104 18 3.478227424855689 1104 19 4.279770934211154 1104 20 3.621219309008744 1104 21 2.486467125094176 1104 22 5.068706061915609 1104 23 5.160627663611432 1104 24 6.477366554878866 1104 25 5.316812705887273 1105 1 3.0093416420261327 1105 2 9.027918248076872 1105 3 6.0475127279226015 1105 4 5.228198768685843 1105 5 2.2925235382481546 1105 6 4.545444100075123 1105 7 5.470007202296326 1105 8 2.4454549533386825 1105 9 3.532851813002818 1105 10 2.98336036079926 1105 11 6.334681350310127 1105 12 3.8512256685940223 1105 13 4.296065422600897 1105 14 2.8808226347468953 1105 15 3.4524657575637967 1105 16 3.856830844514885 1105 17 4.553621762762116 1105 18 1.8329729503329233 1105 19 1.7906532316788173 1105 20 0.981925901422656 1105 21 2.1652594258376965 1105 22 3.52517190862536 1105 23 0.6566891901607654 1105 24 3.892030481737912 1105 25 4.459992471018257 1106 1 6.487119582275557 1106 2 11.237991652767754 1106 3 8.102683225670745 1106 4 9.131250311191678 1106 5 8.764244535434145 1106 6 7.977235252519019 1106 7 7.721891873421217 1106 8 7.330731285118787 1106 9 6.602458729095957 1106 10 6.010995163526186 1106 11 8.31383542542402 1106 12 5.484764366091309 1106 13 4.35421889981912 1106 14 6.053253795509342 1106 15 6.036236139632127 1106 16 6.144007607169801 1106 17 6.563564215304147 1106 18 6.618159435291297 1106 19 6.417117101233579 1106 20 5.483092447860301 1106 21 5.7183892796782025 1106 22 6.20396708386728 1106 23 6.752222519594778 1106 24 7.668353325901606 1106 25 6.323707190299743 1107 1 3.96575695209998 1107 2 -13.451756548671096 1107 3 -5.311589551911384 1107 4 2.0958828010040236 1107 5 4.396105480244728 1107 6 4.819502697304451 1107 7 4.854193025506543 1107 8 5.207309742632496 1107 9 3.4094089730965607 1107 10 3.014655172581879 1107 11 5.727743459835651 1107 12 2.963169054927654 1107 13 4.242160993696466 1107 14 3.8090582428605515 1107 15 3.6170896386510947 1107 16 3.5160267750522785 1107 17 4.280907124193821 1107 18 -0.9994199277916636 1107 19 1.8191898486300673 1107 20 2.1885686263580624 1107 21 -0.965164576537525 1107 22 2.0282048964144437 1107 23 -0.795999043015275 1107 24 2.3168603943727035 1107 25 3.040006179080745 1108 1 5.396370028344123 1108 2 10.414086346298014 1108 3 0.2519415771537261 1108 4 6.093693242989222 1108 5 7.3640853159430115 1108 6 7.338903199714661 1108 7 7.557295748026739 1108 8 7.479366569300975 1108 9 6.15288273234702 1108 10 5.2966838235425655 1108 11 7.335617382029962 1108 12 3.648025460657845 1108 13 4.019513495563533 1108 14 5.536661949833192 1108 15 5.3363085265593035 1108 16 5.21655803990393 1108 17 0.163587550563566 1108 18 3.825084451324365 1108 19 5.054787988354423 1108 20 5.403943057751913 1108 21 5.268541038236052 1108 22 1.9836584234699117 1108 23 5.006567322657887 1108 24 5.304571712181108 1108 25 6.103367574966413 1109 1 3.16183544529299 1109 2 -13.451756548671096 1109 3 -5.400873351970658 1109 4 1.8857978003419262 1109 5 1.346285034684879 1109 6 2.0003230041391866 1109 7 3.4193654545202676 1109 8 3.477529716526952 1109 9 3.0504001955801208 1109 10 2.5863287649948745 1109 11 0.19034206501766704 1109 12 1.3017198188616455 1109 13 2.4382355458350418 1109 14 3.240333347377316 1109 15 3.1866957539064416 1109 16 3.075709315235269 1109 17 3.5821361026094536 1109 18 3.7120089724334484 1109 19 2.2570654311179155 1109 20 0.9804100131047697 1109 21 0.5450900471075393 1109 22 2.5736192431137326 1109 23 3.072823748260177 1109 24 4.068864026448473 1109 25 4.526160466632689 1110 1 2.729517177114815 1110 2 8.383114300037391 1110 3 5.815032903124354 1110 4 6.765385807222895 1110 5 7.693679216471601 1110 6 3.2610362559966823 1110 7 5.7959846287372585 1110 8 6.515043982562311 1110 9 4.948854306361085 1110 10 4.420668900551323 1110 11 2.214599316156452 1110 12 4.85041649383561 1110 13 9.448586947816146 1110 14 6.881726810455319 1110 15 5.999050390076789 1110 16 5.751695626496037 1110 17 6.08899294980732 1110 18 6.174498865592074 1110 19 5.312896787447709 1110 20 5.442103836535338 1110 21 2.8136267428441997 1110 22 5.010067590915515 1110 23 5.599796121230074 1110 24 6.574459806031157 1110 25 6.834647884239054 1111 1 5.433203298856672 1111 2 9.787854415077799 1111 3 7.837855830292618 1111 4 8.486667303857491 1111 5 8.914502313296733 1111 6 8.033090833306382 1111 7 7.871474594512247 1111 8 8.13763085829844 1111 9 6.420149669182084 1111 10 5.72279760486224 1111 11 7.465656408955496 1111 12 6.7656166073752075 1111 13 8.449027403149085 1111 14 6.512448243664348 1111 15 6.230655670320573 1111 16 5.877020666218584 1111 17 7.253417372566137 1111 18 6.820781422582101 1111 19 5.803927811816308 1111 20 5.425627544451462 1111 21 8.260560847463761 1111 22 7.61424098676088 1111 23 7.6141769878504135 1111 24 7.467009096245544 1111 25 8.037856030378872 1112 1 -1.9628444465014212 1112 2 7.572788905874358 1112 3 5.813795063473229 1112 4 -0.31859272347150025 1112 5 3.7614551305943777 1112 6 2.7285236763254304 1112 7 5.470067151380668 1112 8 5.6827293230520795 1112 9 4.313429952117541 1112 10 2.9075223054490102 1112 11 -0.5005432534510632 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4.065533006686259 2204 7 5.109866507338062 2204 8 4.94707826203317 2204 9 3.49299398481509 2204 10 3.1031824491200517 2204 11 4.890736574804244 2204 12 4.648869594170428 2204 13 2.644839264562748 2204 14 4.117796167061691 2204 15 4.14385249886082 2204 16 3.957458753609597 2204 17 -0.8894910516539483 2204 18 2.6617169598438615 2204 19 3.403956240276571 2204 20 3.5814203250680734 2204 21 5.065625931773418 2204 22 4.78749317852289 2204 23 3.7225568788104013 2204 24 5.287738743997647 2204 25 5.425350398893631 2205 1 5.8415512562738705 2205 2 10.27519063127519 2205 3 7.133187917709414 2205 4 7.668115179189847 2205 5 7.642664538912115 2205 6 7.218947701718122 2205 7 7.362748033613922 2205 8 7.225987237921451 2205 9 5.993734338867764 2205 10 5.363360182880442 2205 11 7.445369499865453 2205 12 6.774224829949411 2205 13 9.57657870221892 2205 14 7.2832280971781245 2205 15 6.1071706185485475 2205 16 5.721361838377494 2205 17 6.546560003096474 2205 18 6.573577017071887 2205 19 4.642269745456609 2205 20 5.452466992986956 2205 21 -0.5400576780644677 2205 22 3.8399798493039903 2205 23 5.163820904271102 2205 24 6.880877397959985 2205 25 7.448266003410399 2206 1 4.01782819923849 2206 2 8.80564255446792 2206 3 6.372139947288968 2206 4 2.1610322255704872 2206 5 5.962914568017943 2206 6 6.042164116602642 2206 7 6.199471961816869 2206 8 6.184722800426723 2206 9 4.943474795166868 2206 10 3.897053491751496 2206 11 3.4465297184201162 2206 12 4.79597190230449 2206 13 8.156753016023917 2206 14 0.7936383488532144 2206 15 2.6238059044480764 2206 16 3.400279181838318 2206 17 4.58455414981394 2206 18 -0.7406559339379832 2206 19 0.9798949507028851 2206 20 2.0368815242225295 2206 21 3.1741640320735947 2206 22 -2.165489637750941 2206 23 -2.921810451337894 2206 24 1.3444373017702382 2206 25 2.6227917791309174 2207 1 1.8354152469160745 2207 2 -13.451756548671096 2207 3 -5.914849907426023 2207 4 -3.9582058065110957 2207 5 1.415716955334112 2207 6 2.0787720573402666 2207 7 3.3607540929332522 2207 8 3.3783412584566186 2207 9 0.8971089421986953 2207 10 0.8424153294673715 2207 11 -0.8168716410238126 2207 12 -1.6054037158543153 2207 13 0.8566304962408111 2207 14 -5.200604434408074 2207 15 -0.9458560946057304 2207 16 0.11087613218747805 2207 17 1.2412884921495788 2207 18 1.2692677641376875 2207 19 0.6006181813433429 2207 20 0.1607526641417394 2207 21 -3.6731245606471923 2207 22 -3.722142704586897 2207 23 -1.0416938351482976 2207 24 0.7415421148163723 2207 25 1.3674589337201546 2208 1 4.989769482991271 2208 2 6.244718085894682 2208 3 6.458274075432897 2208 4 8.264110317253895 2208 5 3.6015536219568425 2208 6 6.281385397579239 2208 7 6.813491680419986 2208 8 6.865906842007757 2208 9 5.190558069056493 2208 10 4.699933780474414 2208 11 6.8927007080904055 2208 12 6.430541536272621 2208 13 8.861556967456156 2208 14 3.3524706558989337 2208 15 5.064941979775146 2208 16 5.373266160774037 2208 17 6.392338255401391 2208 18 0.9342855541726323 2208 19 3.3951516125754964 2208 20 4.605252329585188 2208 21 7.2585530214277085 2208 22 6.253793259040641 2208 23 2.370197615012856 2208 24 3.9066781432618214 2208 25 5.378005024603237 2209 1 3.1443648558241613 2209 2 4.378010893189369 2209 3 4.9648732875747115 2209 4 0.39430368244816094 2209 5 4.828389591482328 2209 6 5.339517333830717 2209 7 5.7579952697739 2209 8 4.008120443559477 2209 9 4.059011347466377 2209 10 3.241898626802076 2209 11 -0.8772662978485251 2209 12 2.6116273408277983 2209 13 1.738235965236555 2209 14 3.3141639511057974 2209 15 3.747184776071812 2209 16 2.9379661147823164 2209 17 0.9759859988075767 2209 18 2.870540228331283 2209 19 3.5311015415882743 2209 20 3.7502030384575917 2209 21 5.822269152524111 2209 22 4.312474696381919 2209 23 0.24469700512263515 2209 24 -3.597955022768293 2209 25 -0.6042725654305592 2210 1 -2.050821974591308 2210 2 -13.451756548671096 2210 3 -7.473018011092652 2210 4 -2.0732130843839975 2210 5 -0.7843671370256402 2210 6 -1.1521062181246897 2210 7 -1.0687071080677484 2210 8 -20.269088858766104 2210 9 -12.872337838144631 2210 10 -7.2951913479388 2210 11 -9.664935490427569 2210 12 -4.774861640968211 2210 13 -4.811612480020827 2210 14 -4.231161368988271 2210 15 -6.008761570065282 2210 16 -5.561573994964224 2210 17 -4.910457690766499 2210 18 -5.089867401666105 2210 19 -9.979849595072297 2210 20 -7.287473122155596 2210 21 -5.670908271045263 2210 22 -9.770893477122131 2210 23 -7.79520021637375 2210 24 -10.308450624196741 2210 25 -7.796483174262541 2211 1 0.17300513567128206 2211 2 4.907574927652025 2211 3 0.7512290129963155 2211 4 1.6709472444528686 2211 5 1.2448703411962185 2211 6 0.39975183535303316 2211 7 -20.110841939528424 2211 8 -12.849404550502408 2211 9 -8.772085368216722 2211 10 -6.781151620439399 2211 11 -9.122993662837404 2211 12 -4.6464253896250085 2211 13 -6.077193630323896 2211 14 -4.535575419670046 2211 15 -5.168626154376678 2211 16 -11.587034856909076 2211 17 -7.567395919095297 2211 18 -5.602277028907817 2211 19 -8.314051445822926 2211 20 -6.413415972144641 2211 21 -4.030174543358076 2211 22 -5.525822900301375 2211 23 -7.328824620409097 2211 24 -21.053768976256666 2211 25 -20.849853960779395 2212 1 -1.645053748827003 2212 2 -13.451756548671096 2212 3 -8.014739115526064 2212 4 -19.133138178016956 2212 5 -19.39672668758744 2212 6 -20.224203596401843 2212 7 -9.928671396892765 2212 8 -9.523933820006414 2212 9 -5.613508032378583 2212 10 -5.956512225911101 2212 11 -5.948545367615967 2212 12 -3.3865897134357645 2212 13 -6.191337678174298 2212 14 -4.161960079901956 2212 15 -4.104985766563846 2212 16 -4.250832334830086 2212 17 -4.669091520572266 2212 18 -3.629149694149337 2212 19 -4.206844194845836 2212 20 -4.144486884023028 2212 21 -2.145017669181314 2212 22 -3.506129954780871 2212 23 -3.563752607280467 2212 24 -2.522761224318682 2212 25 -2.8430710150429626 2213 1 -17.93284444650142 2213 2 -13.451756548671096 2213 3 -8.35977596508753 2213 4 -5.294329143109358 2213 5 -19.39672668758744 2213 6 -10.339193329461391 2213 7 -20.110841939528424 2213 8 -10.28654265753407 2213 9 -7.854126516054944 2213 10 -6.810289902876544 2213 11 -6.351349674955635 2213 12 -4.848741313964236 2213 13 -18.37060124406577 2213 14 -11.679111441664368 2213 15 -6.385173755039403 2213 16 -5.871566381278043 2213 17 -4.49554640174636 2213 18 -4.490012753856767 2213 19 -5.502673349773172 2213 20 -5.089640331009004 2213 21 -3.53796292808331 2213 22 -4.223717462540769 2213 23 -4.12955255316016 2213 24 -3.270401419788495 2213 25 -4.189689689732166 2214 1 -17.93284444650142 2214 2 -13.451756548671096 2214 3 -12.012228596337064 2214 4 -19.133138178016956 2214 5 -19.39672668758744 2214 6 -20.224203596401843 2214 7 -15.983057648789854 2214 8 -14.53051910495602 2214 9 -13.482466882583514 2214 10 -13.480741576038787 2214 11 -13.768275322085827 2214 12 -12.057270938841766 2214 13 -18.37060124406577 2214 14 -16.11632542973883 2214 15 -22.917001270439815 2214 16 -17.618550693200454 2214 17 -22.635176791890096 2214 18 -17.0756676835529 2214 19 -14.321765766680764 2214 20 -13.137063244701709 2214 21 -10.79193188894376 2214 22 -11.900933520854101 2214 23 -11.7309304656372 2214 24 -10.61531645574084 2214 25 -10.341061463710227 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 124584.0 3 3.0 4 660.0 5 2348.0 6 3127.0 7 3017.0 8 2565.0 9 2435.0 10 2267.0 11 2194.0 12 2127.0 13 2134.0 14 3167.0 15 6316.0 16 9223.0 17 9907.0 18 10200.0 19 12108.0 20 14676.0 21 16910.0 22 17533.0 23 18948.0 24 19834.0 25 19878.0 26 16806.0 27 13988.0 28 14804.0 29 16891.0 30 16409.0 31 18566.0 32 23625.0 33 28907.0 34 34771.0 35 39159.0 36 37987.0 37 38363.0 38 25455.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.09771970381795 18.962089643995874 13.072643175572074 25.867547476614096 2 12.883993156348627 20.392392521647146 43.041749749819346 23.681864572184878 3 18.25878647770295 25.349616376855295 29.938184742185143 26.45341240325661 4 10.945860427576973 17.277338053616795 39.3447855236488 32.43201599515743 5 12.33881153867885 38.073557528446635 36.92840179419275 12.659229138681768 6 30.765924174655467 37.9853033273178 18.97356748203678 12.27520501598995 7 26.413764344401734 31.24161407561596 24.027232771450954 18.317388808531355 8 23.541044910287294 38.66815288914459 21.05984100159067 16.73096119897745 9 26.46253675352327 15.114411183209977 20.39345280340667 38.02959925986008 10 14.10912711156786 29.669214464641275 35.67329308240735 20.54836534138352 11 33.00512830822791 21.81147729119442 26.034080380912172 19.149314019665496 12 23.97928056780118 24.50516557226339 32.64470241026425 18.870851449671175 13 28.573336694496597 21.624570605122262 25.59118221392801 24.210910486453134 14 19.479084578394634 23.261068849611604 28.395438974573413 28.86440759742035 15 21.810901457938808 33.0053813033119 23.511830066850713 21.67188717189858 16 20.539623342711188 28.04978630876509 30.126552154679203 21.284038193844516 17 20.175207074755452 28.857194992407802 30.180453185790533 20.78714474704621 18 20.19741556893155 27.350717129809652 32.67624553701984 19.775621764238956 19 22.7569018097026 26.633252984186583 27.69074625139915 22.919098954711668 20 23.115780452774494 28.066940161716513 28.686033501821097 20.1312458836879 21 21.605546771109687 26.265125602873944 26.969719022699156 25.159608603317213 22 22.09562874492299 29.305344432380267 28.149274138416374 20.449752684280373 23 20.913343863223382 28.741371853508845 29.043327276270205 21.301957006997572 24 22.430653813127712 27.46574842430584 29.32285999353383 20.780737769032612 25 21.861176947743317 27.803267468535726 29.30407863217531 21.031476951545645 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 535.0 1 535.0 2 1281.0 3 2027.0 4 2027.0 5 2027.0 6 2848.0 7 3669.0 8 3669.0 9 3669.0 10 4274.5 11 4880.0 12 4880.0 13 4880.0 14 6136.0 15 7392.0 16 7392.0 17 7392.0 18 10854.0 19 14316.0 20 14316.0 21 14316.0 22 20630.5 23 26945.0 24 26945.0 25 26945.0 26 35764.5 27 44584.0 28 44584.0 29 44584.0 30 54038.0 31 63492.0 32 63492.0 33 63492.0 34 69123.0 35 74754.0 36 74754.0 37 74754.0 38 77299.0 39 79844.0 40 79844.0 41 79844.0 42 79606.0 43 79368.0 44 79368.0 45 79368.0 46 76756.0 47 74144.0 48 74144.0 49 74144.0 50 67093.5 51 60043.0 52 60043.0 53 60043.0 54 50548.5 55 41054.0 56 41054.0 57 41054.0 58 33670.0 59 26286.0 60 26286.0 61 26286.0 62 20811.0 63 15336.0 64 15336.0 65 15336.0 66 11494.5 67 7653.0 68 7653.0 69 7653.0 70 5566.0 71 3479.0 72 3479.0 73 3479.0 74 2432.5 75 1386.0 76 1386.0 77 1386.0 78 942.0 79 498.0 80 498.0 81 498.0 82 326.0 83 154.0 84 154.0 85 154.0 86 98.0 87 42.0 88 42.0 89 42.0 90 26.0 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.991935330721072 2 36.26933083501611 3 5.5313249732549234 4 13.724971988884176 5 13.172029397428675 6 14.587619403315758 7 9.298899178973622 8 7.574237369677096 9 0.10444822849474276 10 1.4451836706272592 11 5.540345502079469 12 5.838814227747779 13 15.87771328011749 14 1.598690915536199 15 2.8938489488710095 16 2.9103074576035146 17 2.865046558589126 18 1.5113342153405962 19 1.4548372190184398 20 0.28121894247751195 21 4.318459483582638 22 1.61689022807695 23 0.10650554208630589 24 8.9556443189659 25 0.11077842416109082 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 631892.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.89609640330173 #Duplication Level Percentage of deduplicated Percentage of total 1 96.9166426228791 89.06261133556438 2 2.2098591144450688 4.06154852437518 3 0.36838424349578386 1.0155922186123778 4 0.16262846440188844 0.5977968417038745 5 0.07795946322299845 0.358208517394516 6 0.05970427519568006 0.3291953897442882 7 0.03766823539500569 0.24230946538411935 8 0.027890450378073882 0.20504188133399845 9 0.021571696386877795 0.17841192216761495 >10 0.10118378422569495 1.7669517134439507 >50 0.008697641420729924 0.5692805412460639 >100 0.006922507581478058 1.0706917812760948 >500 8.875009716650728E-4 0.5423598677535472 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 864 0.13673222639311783 No Hit GTCCTAAAGTGTGTATTTCTCATTT 722 0.11426003177758225 No Hit GTCCTACAGTGTGCATTTCTCATTT 637 0.10080836598659265 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 1.5825489165870117E-4 0.0 13 0.0 0.0 0.0 1.5825489165870117E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTAGG 40 5.355457E-4 20.055555 1 TAGGACC 220 0.0 17.558773 4 TGTAGGA 580 0.0 16.542028 2 CTGTAGG 550 0.0 15.801348 1 AGGACGG 55 8.945314E-5 15.149044 5 CTACACT 115 5.4569682E-11 14.929197 4 TCTACAC 105 2.730303E-9 14.585858 3 GTCTTAC 55 0.0045137582 14.585858 1 TTTAGGA 445 0.0 14.476266 2 GTGTAAG 65 8.2113215E-4 14.398861 1 GTAGGAC 610 0.0 14.167412 3 TCAGGAC 55 0.0010595125 13.922866 3 TTTAATC 65 2.436603E-4 13.463869 3 CCTTATA 60 0.008034303 13.37037 1 TAGGACA 225 0.0 13.353338 4 GGACGGG 55 0.001541397 13.248229 6 CTTTAGG 435 0.0 13.216688 1 GCTCGGC 45 0.009693044 12.997124 8 GATATAC 175 9.276846E-11 12.98836 1 TTAGGAC 455 0.0 12.983017 3 >>END_MODULE