FastQCFastQC Report
Thu 26 May 2016
SRR1926697_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926697_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631892
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGGACGTGAAATATGGCGAGG17870.282801491394099No Hit
GTCCTACAGTGGACATTTCTAAATT17560.27789558975267925No Hit
CTGTAGGACGTGGAATATGGCAAGA17310.2739392174612117No Hit
GTCCTAAAGTGTGTATTTCTCATTT17230.2726731783279421No Hit
GTCCTACAGTGTGCATTTCTCATTT14090.22298114234710992No Hit
CTGTAGGACCTGGAATATGGCGAGA11170.1767707139827692No Hit
CCCATGTACTCTGCGTTGATACCAC10140.16047046014192298No Hit
CTGAAGGACCTGGAATATGGCGAGA9510.1505004019674248No Hit
GTACATGGGAAGCAGTGGTATCAAC8460.1338836383432612No Hit
GTATCAACGCAGAGTACTTTTTTTT6970.1103036594861147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGG250.006118010318.95141218
TCCAACG653.4550558E-614.5780118
CTAGTAC400.005146202614.3029753
TGTCGAG602.6261454E-514.21355918
TCTGGAC603.960067E-412.7137563
TAGGACC4450.012.4270634
TGTAGGA11350.011.5963712
GGCGAGG4300.011.45899519
CTCTAGG1001.8464598E-611.4387351
CTCGAAC752.1199169E-411.37084818
TCGAACT752.1199169E-411.37084819
GTCCTAA4550.011.3130351
AATCCCG1604.7293724E-1111.25240119
GATATAC2800.011.2344721
TAGGACA3150.011.1993214
ATGTCGA855.4469696E-511.14877217
TATGTCG855.4469696E-511.14877216
ATAGTCC600.005709156411.1245363
AGGACCT7200.011.1236495
CTGTAGG11200.011.0642531
Produced by FastQC (version 0.11.5)