FastQCFastQC Report
Thu 26 May 2016
SRR1926696_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926696_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689970
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10750.15580387553082017No Hit
GTCCTAAAGTGTGTATTTCTCATTT9270.13435366755076308No Hit
CTGTAGGACGTGGAATATGGCAAGA7840.11362812875922142No Hit
CTTTAGGACGTGAAATATGGCGAGG7290.10565676768555154No Hit
GTCCTACAGTGTGCATTTCTCATTT7000.10145368639216197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA808.3317354E-716.7073691
TAGGACC2750.016.393544
TGTAGGA6250.015.3322442
CTCTTAC708.3287734E-515.275311
GTCCTAC6450.014.7128471
GGGTAGG550.00452476914.5809771
CTGTTAG550.00452476914.5809771
GTCTTAC1059.098094E-714.0023671
TAGACAG450.006247476713.8899175
AGGACGG450.006247476713.8899175
TGTTAGA600.006874665613.6895042
GGACCTG5650.013.6372886
CTGTAGG6300.013.57805251
GGGAGTG450.007263042513.5775587
GTATAAA802.5909045E-413.3658951
GTCTTAG600.00805379113.3658951
GTAGGAC7100.013.24902253
TAGGACT1151.281478E-813.0673114
TCCTACA7550.013.0548912
GACCTGC550.001819069812.9603967
Produced by FastQC (version 0.11.5)