##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926696_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 689970 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.509574329318667 27.0 2.0 30.0 2.0 31.0 2 16.05312404887169 16.0 2.0 30.0 2.0 31.0 3 19.616276069974056 22.0 15.0 31.0 2.0 31.0 4 21.74118005130658 28.0 2.0 35.0 2.0 37.0 5 22.100230444801948 28.0 2.0 35.0 2.0 37.0 6 22.960814238300216 30.0 2.0 35.0 2.0 37.0 7 22.834552226908418 30.0 2.0 35.0 2.0 37.0 8 22.96655651695001 30.0 2.0 35.0 2.0 37.0 9 25.60121599489833 31.0 17.0 37.0 2.0 39.0 10 26.23153905242257 31.0 18.0 37.0 2.0 39.0 11 23.380657130020147 30.0 7.0 37.0 2.0 39.0 12 24.45227908459788 30.0 8.0 37.0 2.0 39.0 13 21.15472121976318 27.0 2.0 35.0 2.0 39.0 14 24.44627592504022 30.0 10.0 37.0 2.0 40.0 15 25.511864283954374 31.0 10.0 38.0 2.0 40.0 16 25.886266069539257 31.0 10.0 38.0 2.0 40.0 17 25.201240633650738 31.0 8.0 38.0 2.0 40.0 18 25.288803861037437 31.0 10.0 38.0 2.0 40.0 19 25.66158963433193 31.0 8.0 38.0 2.0 40.0 20 25.894501210197546 31.0 2.0 39.0 2.0 40.0 21 23.76078815020943 30.0 2.0 37.0 2.0 40.0 22 24.69063147673087 30.0 2.0 38.0 2.0 40.0 23 24.667036247952808 31.0 2.0 38.0 2.0 40.0 24 23.750910909169964 31.0 2.0 38.0 2.0 40.0 25 23.554527008420656 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.911102586496867 1101 2 9.47856966717875 1101 3 6.362000353348062 1101 4 7.715230732017144 1101 5 7.365057649010705 1101 6 6.909912994992862 1101 7 6.7130308667039635 1101 8 5.219907137347398 1101 9 4.9681145139471035 1101 10 4.35447634666377 1101 11 7.248250892531544 1101 12 4.5547908411267635 1101 13 9.284207776576235 1101 14 6.589647827671236 1101 15 5.914819080453608 1101 16 5.3547716714801155 1101 17 1.3081883125786717 1101 18 3.9624483047533268 1101 19 4.575381438450627 1101 20 2.126884207925393 1101 21 0.2005621010974039 1101 22 3.768171942183244 1101 23 4.745754171819886 1101 24 5.9636270234089395 1101 25 6.587062710228615 1102 1 5.287573174732163 1102 2 10.34075454112833 1102 3 6.473765059230413 1102 4 7.723634093361682 1102 5 7.653292943128353 1102 6 6.533442406757565 1102 7 6.550845992754386 1102 8 6.673688649952439 1102 9 5.751307791258025 1102 10 5.137669623974691 1102 11 7.804553413539946 1102 12 5.009412689866259 1102 13 9.337148953046821 1102 14 6.7720007688477075 1102 15 4.585407315747727 1102 16 5.1488893185389415 1102 17 6.016591673923209 1102 18 5.805305447610468 1102 19 4.7636167325682734 1102 20 5.221842191118672 1102 21 4.158545294374715 1102 22 5.210188748905935 1102 23 5.553317197029973 1102 24 6.903963157862723 1102 25 6.238323214430295 1103 1 4.243587539937703 1103 2 8.525845672175343 1103 3 5.719327743763685 1103 4 7.2857423150287275 1103 5 6.339844726003665 1103 6 6.23377882510869 1103 7 6.344442688189314 1103 8 5.942354900236335 1103 9 4.989523212561682 1103 10 4.192931661222023 1103 11 4.881026840748667 1103 12 3.881512678510365 1103 13 7.806049418638466 1103 14 5.922384030260378 1103 15 5.002341792241026 1103 16 4.758827145020884 1103 17 2.3640891188471223 1103 18 1.273115747406063 1103 19 3.320959737337752 1103 20 3.5284669356110605 1103 21 5.8711403999845295 1103 22 1.5654649810204013 1103 23 3.601230551560974 1103 24 5.163871175123681 1103 25 1.6061888742077208 1104 1 5.3519551923949145 1104 2 9.924197493861207 1104 3 6.787425871602899 1104 4 7.235579992264537 1104 5 7.065648309723208 1104 6 6.857208653493952 1104 7 6.9228637981787635 1104 8 6.78421639245861 1104 9 5.653764597410028 1104 10 4.623968863534692 1104 11 7.634849742597886 1104 12 5.055365807955599 1104 13 9.090422554507779 1104 14 6.545436730668769 1104 15 5.824202596168892 1104 16 5.558339889938978 1104 17 5.899336962191317 1104 18 3.3849105324287763 1104 19 4.4001092922407 1104 20 3.621746601099982 1104 21 3.048549717196476 1104 22 4.921813636551995 1104 23 5.142840816324888 1104 24 6.081774859793068 1104 25 5.2859512979217556 1105 1 3.6625169550611965 1105 2 9.599589906260299 1105 3 6.5817896775284375 1105 4 6.252477743497792 1105 5 2.306577355484844 1105 6 5.078820558018069 1105 7 6.364362385226869 1105 8 3.273286235465516 1105 9 4.2125339718711174 1105 10 4.134036057872194 1105 11 7.038983523612146 1105 12 4.220041166609068 1105 13 5.147694579748208 1105 14 4.056602496862855 1105 15 4.62323427751835 1105 16 4.9840414075435575 1105 17 5.468479471295677 1105 18 2.857489137988029 1105 19 2.9444783729990966 1105 20 2.283600979140921 1105 21 2.698987950310329 1105 22 4.194861491242307 1105 23 1.6896883652155559 1105 24 4.7257763901979075 1105 25 5.53708046380892 1106 1 6.485954159442617 1106 2 11.251114299211284 1106 3 7.89226423136423 1106 4 8.935322593988644 1106 5 8.542523754991432 1106 6 7.82946310687603 1106 7 7.463703204889033 1106 8 7.215002290000378 1106 9 6.222579795525519 1106 10 5.5755789674317775 1106 11 8.1503181332778 1106 12 5.361153979951954 1106 13 4.3512877990221455 1106 14 5.778151225732852 1106 15 5.974530747478209 1106 16 5.973178011757366 1106 17 6.194655644771235 1106 18 6.287661852662186 1106 19 6.2948664294791925 1106 20 5.551190220520045 1106 21 5.475611807707171 1106 22 6.157305765539462 1106 23 6.750242661362449 1106 24 7.611463140543346 1106 25 6.433745356906414 1107 1 3.9420989850562904 1107 2 -13.51722865214898 1107 3 -5.384074076423849 1107 4 1.8229858340579597 1107 5 4.523400986345639 1107 6 4.91521911744184 1107 7 4.779465440533496 1107 8 5.290351315018466 1107 9 3.41870274924122 1107 10 3.0269133214536836 1107 11 5.466042008978121 1107 12 3.1706611892660206 1107 13 4.410450273575034 1107 14 3.7763705167468657 1107 15 3.4349631380766574 1107 16 3.4023827158978825 1107 17 3.8672999572365327 1107 18 -1.2801447324615616 1107 19 1.4563658322081316 1107 20 1.867892611286738 1107 21 -1.7449841173899898 1107 22 1.3722775844401482 1107 23 -1.4585915664754303 1107 24 1.940481765305698 1107 25 2.7697037666511832 1108 1 5.355322436599071 1108 2 10.578955767564853 1108 3 0.3740376006794257 1108 4 6.042598435855435 1108 5 7.435532325367735 1108 6 7.473097187588817 1108 7 7.421797616647186 1108 8 7.117136299282443 1108 9 5.574678220510808 1108 10 4.583184045959705 1108 11 7.144639718332133 1108 12 2.929853298542163 1108 13 3.516254108622565 1108 14 5.1385008824066425 1108 15 5.1113441769787045 1108 16 4.986023090966828 1108 17 0.7246316466690637 1108 18 3.96980692387665 1108 19 5.042790023506271 1108 20 5.229927587439359 1108 21 4.882120129714259 1108 22 1.9892030573320092 1108 23 4.867625623103105 1108 24 5.732034927110963 1108 25 6.089197618245876 1109 1 3.384767377956024 1109 2 -13.51722865214898 1109 3 -5.422724889002636 1109 4 1.982080243107056 1109 5 1.4002297144980034 1109 6 2.5076234648235314 1109 7 3.632517125314255 1109 8 3.685262561654014 1109 9 3.3291630402023458 1109 10 2.427118425198021 1109 11 0.799773738731794 1109 12 0.8629837677130894 1109 13 2.19699519538349 1109 14 3.133098396546533 1109 15 3.374332835872302 1109 16 3.348203301227173 1109 17 3.7912861927992054 1109 18 3.840210962404033 1109 19 2.4198280651814557 1109 20 1.1922999568626977 1109 21 0.6455095625527711 1109 22 2.9326182883343925 1109 23 3.4954658762011412 1109 24 4.528358157932868 1109 25 4.895192506387481 1110 1 2.971540756802213 1110 2 8.963333796155403 1110 3 6.293564045822567 1110 4 7.198794480097021 1110 5 8.116669162102294 1110 6 3.6885612284798057 1110 7 6.399791228416888 1110 8 7.278883581523083 1110 9 5.559428260289312 1110 10 5.103595349103454 1110 11 2.9557325948899056 1110 12 5.7301514071894175 1110 13 10.144926058926394 1110 14 7.629042146192688 1110 15 6.8612057740888766 1110 16 6.545889408897665 1110 17 6.8031504661746 1110 18 7.074693302007812 1110 19 5.758931285188332 1110 20 6.2782482771262735 1110 21 4.134070591342137 1110 22 6.181750078152273 1110 23 6.529349892986765 1110 24 7.619169836067503 1110 25 8.145752786755505 1111 1 5.426113202354003 1111 2 9.889966768946685 1111 3 7.864930879605989 1111 4 8.633324412661928 1111 5 8.986224087785725 1111 6 8.075068934719528 1111 7 8.010043310275016 1111 8 8.173643987770312 1111 9 6.691815703335369 1111 10 5.923551564649571 1111 11 7.803331043970697 1111 12 7.081681597429284 1111 13 8.81100027607808 1111 14 6.828995210123807 1111 15 6.438982695204494 1111 16 6.214780466494442 1111 17 7.398927437337324 1111 18 7.056833924904904 1111 19 6.096063464327209 1111 20 5.788285088801047 1111 21 8.373656228364013 1111 22 7.95952740505523 1111 23 7.770805533329334 1111 24 7.363471736437241 1111 25 8.072713094666938 1112 1 -1.8436967980456807 1112 2 7.457916335423516 1112 3 5.794714283990739 1112 4 0.24833524142654184 1112 5 3.907240155816247 1112 6 2.882465959840772 1112 7 5.657651531871441 1112 8 5.750502474073638 1112 9 4.31112552116689 1112 10 3.137350059529197 1112 11 -0.03889551532737556 1112 12 4.03114307216002 1112 13 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7 4.208233771986681 2204 8 3.904788124918305 2204 9 2.552509880133776 2204 10 2.1128621130306158 2204 11 3.917314698958638 2204 12 3.811162334447708 2204 13 2.143302972443525 2204 14 2.962367166663114 2204 15 3.1419955791938072 2204 16 2.822163251227593 2204 17 -2.42642171233582 2204 18 1.596267259470892 2204 19 2.4060544111572995 2204 20 2.772348222677021 2204 21 4.18634395152602 2204 22 3.5403690622372963 2204 23 2.5272259565136643 2204 24 4.282114418366923 2204 25 4.360571711819201 2205 1 6.188622212768429 2205 2 10.671916084693127 2205 3 7.639691363962832 2205 4 8.06081343878406 2205 5 8.26776524520708 2205 6 7.741891378451509 2205 7 7.934880988331564 2205 8 7.665105611473891 2205 9 6.212762899617161 2205 10 5.6158512913784975 2205 11 7.950639216282095 2205 12 7.307832913218757 2205 13 10.320340848225946 2205 14 7.757554727273181 2205 15 6.911214480718979 2205 16 6.483822699786174 2205 17 7.078082717709144 2205 18 6.890136551104497 2205 19 5.4482045808654895 2205 20 5.781445242865686 2205 21 -0.5567134406982603 2205 22 4.0403938572650695 2205 23 5.341562166070219 2205 24 7.2681341552090295 2205 25 7.76979573765011 2206 1 3.843232922074808 2206 2 8.154956778314595 2206 3 5.771580741794828 2206 4 1.3158456799830844 2206 5 5.0675049193095525 2206 6 5.588413468029756 2206 7 5.882091471078166 2206 8 5.899431596137536 2206 9 4.750363108749262 2206 10 3.87832297140638 2206 11 3.4099891639990716 2206 12 4.706681597429284 2206 13 7.856574074088616 2206 14 0.25862217237600404 2206 15 2.4662423649992142 2206 16 3.2900323982873942 2206 17 4.394645544478053 2206 18 -0.3690837782785614 2206 19 1.2671909437096858 2206 20 2.7159389689026305 2206 21 3.757768957349839 2206 22 -1.8046632183710436 2206 23 -2.825977699736683 2206 24 1.0056243521417585 2206 25 2.5333076320383974 2207 1 1.3155222623169855 2207 2 -13.51722865214898 2207 3 -6.153115148919792 2207 4 -4.342151216578195 2207 5 0.9129321963191686 2207 6 1.5699847224286039 2207 7 2.8898847044733813 2207 8 3.0208986636650366 2207 9 0.7703823666948963 2207 10 0.4945522933604387 2207 11 -0.917583642119137 2207 12 -2.0706196578008402 2207 13 0.4414877596991893 2207 14 -5.1228763451778185 2207 15 -1.265673869864596 2207 16 -0.22570369858422623 2207 17 0.8068029885745922 2207 18 0.8578246979884447 2207 19 0.42143115540854126 2207 20 0.22990097948757438 2207 21 -3.383932114999073 2207 22 -3.717180190301548 2207 23 -1.0165080552466712 2207 24 0.7645411966096027 2207 25 1.2673657938051406 2208 1 4.976268927005673 2208 2 6.372376504982366 2208 3 6.4618046586318165 2208 4 8.183700859794282 2208 5 4.1726667200363785 2208 6 6.540544973811787 2208 7 6.948704862329613 2208 8 6.686696592855629 2208 9 5.2743537219590735 2208 10 4.765262578964908 2208 11 6.693249165813459 2208 12 6.117136875430894 2208 13 8.768241390021611 2208 14 3.0850892919281705 2208 15 4.646625227569988 2208 16 5.133648153579806 2208 17 5.81859466690122 2208 18 0.6005972629667475 2208 19 3.380780310328145 2208 20 4.648617832882227 2208 21 7.048910207463241 2208 22 6.242823720702873 2208 23 1.737684642638353 2208 24 3.1278033788826356 2208 25 4.545978331271552 2209 1 3.3497824973639574 2209 2 4.726329120087097 2209 3 5.3714235740078955 2209 4 1.2573041199758173 2209 5 5.36908609330613 2209 6 5.659574555377613 2209 7 6.20088196724393 2209 8 4.464861977138675 2209 9 4.3191088767795165 2209 10 3.5044480560457743 2209 11 -0.03142317160783037 2209 12 3.4216649723253774 2209 13 2.0909150979087556 2209 14 4.006594955649611 2209 15 4.304518501369106 2209 16 3.8347908113056235 2209 17 1.1066885605581618 2209 18 3.40595159135475 2209 19 3.8244890615497447 2209 20 4.189621622093057 2209 21 6.135372134836587 2209 22 4.962694738833079 2209 23 0.25884748894723586 2209 24 -3.4040867453903516 2209 25 -0.27153938397565014 2210 1 -2.4640012773092224 2210 2 -13.51722865214898 2210 3 -7.6251517119646905 2210 4 -2.4354656811469155 2210 5 -1.021829772049177 2210 6 -1.577982974438914 2210 7 -1.4794713628332339 2210 8 -20.343118072736637 2210 9 -12.959583951116231 2210 10 -7.441229165912272 2210 11 -10.169291758696334 2210 12 -4.932123406884781 2210 13 -5.305859000781819 2210 14 -4.159022063063315 2210 15 -5.67355687389707 2210 16 -5.2589107989196044 2210 17 -4.972257014818897 2210 18 -5.077278785392483 2210 19 -9.947812998944693 2210 20 -7.1130219702490685 2210 21 -5.506670300059778 2210 22 -9.690866927894552 2210 23 -7.979525517277494 2210 24 -10.669999472918668 2210 25 -8.373390737759726 2211 1 0.19830940952458675 2211 2 4.622367484813267 2211 3 0.47977283547523797 2211 4 1.5489696006989462 2211 5 0.9917101010362934 2211 6 -0.08360262020844189 2211 7 -20.21890190640528 2211 8 -13.015637826906962 2211 9 -9.13025498310545 2211 10 -7.425844736993575 2211 11 -9.493841168173336 2211 12 -5.115860105819181 2211 13 -6.861258170996825 2211 14 -5.070442477107392 2211 15 -5.553285773428243 2211 16 -11.742318507875208 2211 17 -7.922201753895681 2211 18 -6.069986161570515 2211 19 -8.813995207730233 2211 20 -6.960448038405975 2211 21 -4.614012086786705 2211 22 -6.153726966634011 2211 23 -8.354321082162302 2211 24 -21.217885581633077 2211 25 -21.02050031498147 2212 1 -1.028177931363988 2212 2 -13.51722865214898 2212 3 -7.6082018618136225 2212 4 -19.19905498226857 2212 5 -19.492925544266605 2212 6 -20.332944147864282 2212 7 -9.735686358702099 2212 8 -9.164672843054658 2212 9 -5.13845525176837 2212 10 -5.553591474104113 2212 11 -5.19256331300933 2212 12 -2.8476470244788423 2212 13 -5.540010532655586 2212 14 -3.556456338376808 2212 15 -3.654621487292925 2212 16 -3.689795837502274 2212 17 -3.9031161385303967 2212 18 -3.0095548427954455 2212 19 -3.5049552898802787 2212 20 -3.493626823342236 2212 21 -1.4030961820038215 2212 22 -2.87056369341375 2212 23 -2.9796177437307847 2212 24 -2.0394403519510966 2212 25 -2.644175226642247 2213 1 -18.03259489249473 2213 2 -13.51722865214898 2213 3 -8.619898914149957 2213 4 -5.862666347988094 2213 5 -19.492925544266605 2213 6 -10.694997310100762 2213 7 -20.21890190640528 2213 8 -10.54660111581638 2213 9 -8.432673445599686 2213 10 -7.322826105022681 2213 11 -6.617550276698754 2213 12 -5.159381647300322 2213 13 -18.52587625703721 2213 14 -12.025535991044151 2213 15 -7.097239858612394 2213 16 -6.494332149700053 2213 17 -5.031795050152308 2213 18 -5.106571713886577 2213 19 -5.989588637572449 2213 20 -6.002061559199056 2213 21 -4.156983593624112 2213 22 -5.259402020490732 2213 23 -4.974606456391065 2213 24 -4.093229302989631 2213 25 -4.828932945595586 2214 1 -18.03259489249473 2214 2 -13.51722865214898 2214 3 -12.045179918965573 2214 4 -19.19905498226857 2214 5 -19.492925544266605 2214 6 -20.332944147864282 2214 7 -16.070488395658092 2214 8 -14.568297192490988 2214 9 -13.387719242278008 2214 10 -13.387638513609922 2214 11 -13.892651200137664 2214 12 -11.999178177391595 2214 13 -18.52587625703721 2214 14 -16.13494985172806 2214 15 -23.022996045596813 2214 16 -17.607091518012673 2214 17 -22.72290412439612 2214 18 -17.1015496997709 2214 19 -14.245348286397347 2214 20 -13.097962045826876 2214 21 -10.622112352512083 2214 22 -11.577968274394685 2214 23 -11.655593634446939 2214 24 -10.47581802789715 2214 25 -10.258985473630394 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 134581.0 3 10.0 4 716.0 5 2619.0 6 3413.0 7 3167.0 8 2857.0 9 2703.0 10 2587.0 11 2353.0 12 2289.0 13 2335.0 14 3398.0 15 6969.0 16 10179.0 17 11048.0 18 11579.0 19 13253.0 20 15443.0 21 18219.0 22 18930.0 23 20433.0 24 21395.0 25 21833.0 26 18550.0 27 15025.0 28 15938.0 29 18207.0 30 17910.0 31 20392.0 32 25776.0 33 31634.0 34 38198.0 35 42518.0 36 41704.0 37 42449.0 38 29360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22664414828687 18.905071651923816 12.85035867765831 26.017925522130998 2 12.935473063063679 20.49199196803625 43.084283197377985 23.488251771522084 3 18.264314771344647 25.532464021040468 29.942267749824868 26.260953457790016 4 10.940194210000907 17.384450427376283 39.521970468517345 32.15338489410547 5 12.265007521769576 38.106557843915745 37.07930860028077 12.549126034033915 6 30.420181498819083 38.32151850951279 18.923895984206847 12.334404007461277 7 26.437419619034213 31.354335293402524 23.942650380389924 18.265594707173342 8 23.308870428093005 39.084231690823984 21.021957063761835 16.58494081732118 9 26.353919998142732 15.232071551283838 20.51338974787575 37.90061870269768 10 14.141628029744915 29.7134471192517 35.7453654074927 20.399559443510686 11 32.83204361481773 21.757008426707316 26.26585001567331 19.14509794280165 12 24.12347977261014 24.61979891843658 32.80583398078417 18.45088732816911 13 28.738605631734014 21.892566767757934 25.369069528327152 23.999758072180892 14 19.392786244028297 23.4578137241619 28.312637327034206 28.83676270477559 15 21.62056811732728 33.51578601511395 23.436482571164014 21.427163296394756 16 20.199731578931637 28.207078783675932 30.431864988387353 21.161324649005078 17 20.102298582869018 29.141510053687348 30.26512233429481 20.491069029148825 18 20.104914655679814 27.270306062389643 32.99764567392584 19.62713360800471 19 22.86101682951076 26.666480391061125 27.57442690839358 22.898075871034536 20 23.18944865040877 28.26131830815569 28.823308795311025 19.725924246124503 21 21.480452344445993 26.348730659911396 26.989006526995823 25.181810468646788 22 22.027913828024303 29.652145092984718 28.17838335481495 20.141557724176025 23 20.63516246891224 29.068687624967716 29.20638789978786 21.08976200633218 24 22.38456717263145 27.447290474266424 29.62789158715564 20.54025076594648 25 21.654620198886434 28.1436056981413 29.336111687516414 20.86566241545585 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 729.0 1 729.0 2 1496.0 3 2263.0 4 2263.0 5 2263.0 6 3303.0 7 4343.0 8 4343.0 9 4343.0 10 5023.5 11 5704.0 12 5704.0 13 5704.0 14 7184.5 15 8665.0 16 8665.0 17 8665.0 18 12638.5 19 16612.0 20 16612.0 21 16612.0 22 23425.0 23 30238.0 24 30238.0 25 30238.0 26 40106.0 27 49974.0 28 49974.0 29 49974.0 30 60446.0 31 70918.0 32 70918.0 33 70918.0 34 76643.0 35 82368.0 36 82368.0 37 82368.0 38 84196.5 39 86025.0 40 86025.0 41 86025.0 42 85411.0 43 84797.0 44 84797.0 45 84797.0 46 82344.5 47 79892.0 48 79892.0 49 79892.0 50 72049.0 51 64206.0 52 64206.0 53 64206.0 54 54128.5 55 44051.0 56 44051.0 57 44051.0 58 36263.0 59 28475.0 60 28475.0 61 28475.0 62 22389.0 63 16303.0 64 16303.0 65 16303.0 66 12356.5 67 8410.0 68 8410.0 69 8410.0 70 6046.5 71 3683.0 72 3683.0 73 3683.0 74 2599.0 75 1515.0 76 1515.0 77 1515.0 78 1034.5 79 554.0 80 554.0 81 554.0 82 363.0 83 172.0 84 172.0 85 172.0 86 113.5 87 55.0 88 55.0 89 55.0 90 35.0 91 15.0 92 15.0 93 15.0 94 9.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.325217038421961 2 36.41071350928301 3 5.657347420902358 4 13.76059828688204 5 13.484064524544545 6 14.76397524530052 7 9.394901227589605 8 7.516413757119874 9 0.11391799643462759 10 1.4378886038523415 11 5.678797628882415 12 5.795179500557995 13 16.128962128788206 14 1.58470658144557 15 2.9552009507659753 16 3.025059060538864 17 2.9227357711204833 18 1.5029638969810282 19 1.4455700972506051 20 0.2982738379929562 21 4.339608968505877 22 1.6080409293157671 23 0.11478759946084612 24 8.889661869356638 25 0.12072988680667275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 689970.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.23824643082317 #Duplication Level Percentage of deduplicated Percentage of total 1 96.95578382457353 88.46075697480057 2 2.1095063308921724 3.8493531693064327 3 0.40646688781080176 1.1125597822815505 4 0.16004354692965636 0.58408370297724 5 0.09180426436285966 0.41880300476695137 6 0.0625875510294119 0.3426227042593921 7 0.03711216939629322 0.23702344798730185 8 0.028375774418652678 0.2071164719259585 9 0.01607733793525975 0.13201813084399658 >10 0.11285970343363198 1.9514890281551351 >50 0.009855749375982275 0.6221063611641737 >100 0.008541322620004382 1.37485674109883 >500 8.212810181963868E-4 0.546106164589137 >1k 1.6425620363927736E-4 0.1611043158433925 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1075 0.15580387553082017 No Hit GTCCTAAAGTGTGTATTTCTCATTT 927 0.13435366755076308 No Hit CTGTAGGACGTGGAATATGGCAAGA 784 0.11362812875922142 No Hit CTTTAGGACGTGAAATATGGCGAGG 729 0.10565676768555154 No Hit GTCCTACAGTGTGCATTTCTCATTT 700 0.10145368639216197 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.4493383770308854E-4 0.0 5 0.0 0.0 0.0 1.4493383770308854E-4 0.0 6 0.0 0.0 0.0 1.4493383770308854E-4 0.0 7 0.0 0.0 0.0 1.4493383770308854E-4 0.0 8 0.0 0.0 0.0 1.4493383770308854E-4 0.0 9 0.0 0.0 0.0 1.4493383770308854E-4 0.0 10 0.0 0.0 0.0 1.4493383770308854E-4 0.0 11 0.0 0.0 0.0 1.4493383770308854E-4 0.0 12 0.0 0.0 0.0 1.4493383770308854E-4 0.0 13 0.0 0.0 0.0 1.4493383770308854E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAA 80 8.3317354E-7 16.707369 1 TAGGACC 275 0.0 16.39354 4 TGTAGGA 625 0.0 15.332244 2 CTCTTAC 70 8.3287734E-5 15.27531 1 GTCCTAC 645 0.0 14.712847 1 GGGTAGG 55 0.004524769 14.580977 1 CTGTTAG 55 0.004524769 14.580977 1 GTCTTAC 105 9.098094E-7 14.002367 1 TAGACAG 45 0.0062474767 13.889917 5 AGGACGG 45 0.0062474767 13.889917 5 TGTTAGA 60 0.0068746656 13.689504 2 GGACCTG 565 0.0 13.637288 6 CTGTAGG 630 0.0 13.5780525 1 GGGAGTG 45 0.0072630425 13.577558 7 GTATAAA 80 2.5909045E-4 13.365895 1 GTCTTAG 60 0.008053791 13.365895 1 GTAGGAC 710 0.0 13.2490225 3 TAGGACT 115 1.281478E-8 13.067311 4 TCCTACA 755 0.0 13.054891 2 GACCTGC 55 0.0018190698 12.960396 7 >>END_MODULE