FastQCFastQC Report
Thu 26 May 2016
SRR1926696_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926696_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689970
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA22840.3310288853138542No Hit
GTCCTACAGTGGACATTTCTAAATT21990.3187095091090917No Hit
CTTTAGGACGTGAAATATGGCGAGG21550.3123324202501558No Hit
GTCCTAAAGTGTGTATTTCTCATTT20700.30001304404539325No Hit
GTCCTACAGTGTGCATTTCTCATTT16390.23754655999536212No Hit
CTGTAGGACCTGGAATATGGCGAGA13460.19508094554835717No Hit
GTATCAACGCAGAGTACTTTTTTTT11870.1720364653535661No Hit
CTGAAGGACCTGGAATATGGCGAGA11660.16899285476180123No Hit
CCCATGTACTCTGCGTTGATACCAC11460.16609417800773946No Hit
GTACATGGGAAGCAGTGGTATCAAC11400.16522457498152093No Hit
GTCCTTCAGTGTGCATTTCTCATTT7050.10217835558067742No Hit
GAATATGGCAAGAAAACTGAAAATC7030.10188848790527125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5150.014.6243774
TGTAGGA11400.013.7180232
GTAGGAC11500.013.4299583
TCTATAC500.0014587113.3470583
GGACCTG8200.013.2530796
CTGTAGG11450.012.985241
AGGACCT8550.012.9344925
GGCGAGG6250.012.58447919
ACAGGTC701.08733286E-412.2165288
TGTCGAG701.1149369E-412.18376818
AGAACCG550.002985409512.133695
TAGGACA3150.012.1061744
GCCGGTT550.003106524712.06944811
CTAGGAC959.93643E-712.0424593
ACTGTTC2450.012.02261458
AGGACGT12850.011.9448395
GGACGTG12550.011.8496836
GTATAAG657.798492E-411.7302561
GACGTGG5400.011.6115557
TAGGACG13300.011.5406894