##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926696_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 689970 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.28367755119788 31.0 28.0 31.0 22.0 34.0 2 28.80235227618592 31.0 28.0 33.0 23.0 34.0 3 29.13959737379886 31.0 28.0 33.0 25.0 34.0 4 32.890311172949545 35.0 33.0 37.0 27.0 37.0 5 32.53474788758932 35.0 33.0 37.0 25.0 37.0 6 32.64962389669116 35.0 33.0 37.0 26.0 37.0 7 32.472804614693395 35.0 33.0 37.0 24.0 37.0 8 32.45070945113556 35.0 33.0 37.0 25.0 37.0 9 33.66173311883125 37.0 33.0 39.0 22.0 39.0 10 33.5338275577199 37.0 33.0 39.0 22.0 39.0 11 33.77510906271287 37.0 34.0 39.0 25.0 39.0 12 33.57400466686958 37.0 33.0 39.0 22.0 39.0 13 33.713690450309436 37.0 34.0 39.0 24.0 39.0 14 34.33381741235126 38.0 33.0 40.0 19.0 41.0 15 34.50408423554647 38.0 34.0 40.0 22.0 41.0 16 34.498841978636754 38.0 34.0 40.0 22.0 41.0 17 34.40814238300216 38.0 34.0 40.0 21.0 41.0 18 34.36750293491021 38.0 34.0 40.0 19.0 41.0 19 34.38874733684073 39.0 34.0 40.0 19.0 41.0 20 34.384806585793584 39.0 34.0 40.0 18.0 41.0 21 34.30534515993449 39.0 34.0 40.0 18.0 41.0 22 34.24374972824906 39.0 34.0 40.0 18.0 41.0 23 33.896362160673654 39.0 34.0 40.0 11.0 41.0 24 33.99628679507805 39.0 34.0 40.0 16.0 41.0 25 33.793656245923735 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5656409246653276 1101 2 0.4780813458555784 1101 3 0.5385124227240539 1101 4 0.35220230165388244 1101 5 0.34598326894860065 1101 6 0.1379814330475142 1101 7 0.3075896632891073 1101 8 0.2974475394660985 1101 9 0.7798671302281477 1101 10 0.8034282236216015 1101 11 0.674101732549893 1101 12 0.6217859233203527 1101 13 0.6671778087499831 1101 14 0.8999467107316903 1101 15 0.6320248646595061 1101 16 0.608623200663061 1101 17 0.7120126298893368 1101 18 0.62724156466858 1101 19 0.6670878020872166 1101 20 0.5503125268157234 1101 21 0.3694277750570336 1101 22 0.48644859452453204 1101 23 0.8205105954358203 1101 24 0.6197870984692315 1101 25 0.6209418432779898 1102 1 0.5547165549174267 1102 2 0.5721989929144016 1102 3 0.5023779689425432 1102 4 0.4303535621580892 1102 5 0.5569076386965008 1102 6 0.27159487842566676 1102 7 0.6143123523647347 1102 8 0.547027371398876 1102 9 0.9958335167827741 1102 10 1.0395626774031186 1102 11 0.5749420686843507 1102 12 0.6032985283623731 1102 13 0.6495307499264555 1102 14 0.9881820048493353 1102 15 0.7370668814662267 1102 16 0.6640853855370068 1102 17 0.8758781761078254 1102 18 0.594468455424888 1102 19 0.8897768777174733 1102 20 0.7906486612695076 1102 21 0.8669067666536776 1102 22 0.9351880903228462 1102 23 1.1558047130828797 1102 24 0.985333316956627 1102 25 0.9125384819334528 1103 1 -0.05874301810096938 1103 2 -0.0343079341807595 1103 3 -0.07829004150488217 1103 4 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0.6886044319631495 2202 19 0.684743320670492 2202 20 0.5883123576205307 2202 21 0.6428035011864139 2202 22 0.6959404449559798 2202 23 1.0955049555959917 2202 24 0.8504170685780821 2202 25 0.7361637709752387 2203 1 -0.23649532514302507 2203 2 -0.31283776722840173 2203 3 -0.3345646969161571 2203 4 -0.7949426727563846 2203 5 -0.6366952933538208 2203 6 -0.6563778745330353 2203 7 -0.5715844564022099 2203 8 -0.2933810421837606 2203 9 0.07954515956325281 2203 10 -0.16136746007324376 2203 11 -0.4796973022681996 2203 12 -0.3559119499210368 2203 13 -0.5582899978339881 2203 14 -0.13261467177092356 2203 15 -0.22123317183050517 2203 16 -0.27531331051829966 2203 17 -0.25387673193706206 2203 18 0.09568770941666571 2203 19 -0.1060528355753192 2203 20 -0.09327397815985705 2203 21 -0.12739705391886247 2203 22 -0.11383687152983413 2203 23 -0.07998367699078557 2203 24 -0.0950374238679359 2203 25 -0.04736266358123231 2204 1 0.044574809777159885 2204 2 0.12390429815207682 2204 3 0.1361082449725508 2204 4 -0.018637893720750753 2204 5 0.12288598983595023 2204 6 -0.07646193223830267 2204 7 0.280138682896947 2204 8 0.22570366603733305 2204 9 0.6021254808045597 2204 10 0.36701365779527606 2204 11 0.2649424909638114 2204 12 0.38428046887727163 2204 13 0.246507228960283 2204 14 0.4102742588363597 2204 15 0.5161119668370588 2204 16 0.2306788570964855 2204 17 0.015000960900131588 2204 18 -0.04169555791914803 2204 19 0.16381372863874333 2204 20 0.29368907341426365 2204 21 0.16735719808252725 2204 22 0.14761155216989863 2204 23 0.46170536480084934 2204 24 0.36419400696127013 2204 25 0.29321075084101267 2205 1 0.26342536738978595 2205 2 0.29323918530715076 2205 3 0.22514167968999743 2205 4 -0.05083285977910634 2205 5 -0.04954138828271226 2205 6 -0.07237626266235253 2205 7 -0.1677779837697173 2205 8 -0.007901310600239242 2205 9 0.5956953036027599 2205 10 0.32726585077772796 2205 11 0.285133908578203 2205 12 0.1655525089019534 2205 13 0.1085228994178351 2205 14 0.4613382410280167 2205 15 0.46294591729108703 2205 16 0.09240111972542309 2205 17 0.1647202260857128 2205 18 0.28997099854739616 2205 19 0.26132293034905274 2205 20 0.14804721279537603 2205 21 0.31945265342943685 2205 22 0.250663820088441 2205 23 0.7607347772137913 2205 24 0.3301834938694981 2205 25 0.5629475929462728 2206 1 0.5886639086933734 2206 2 0.646133268611436 2206 3 0.7784812579402605 2206 4 0.6994503399420466 2206 5 0.6603858511735439 2206 6 0.6390541137754369 2206 7 0.7583395658991563 2206 8 0.7751132415085067 2206 9 0.7868986845717387 2206 10 0.8904684039321396 2206 11 0.6096045429455756 2206 12 0.6775667124571996 2206 13 0.5862481521191185 2206 14 0.9198059126906202 2206 15 0.6313626130038728 2206 16 0.8212101559749883 2206 17 0.6465791054861967 2206 18 0.6258349755428654 2206 19 0.5482205863267424 2206 20 0.7122845341617108 2206 21 0.8402678329344937 2206 22 0.678028537760099 2206 23 1.157546601891383 2206 24 1.0802161707604014 2206 25 1.156621346867503 2207 1 -0.9907952062724057 2207 2 0.03029118376563744 2207 3 0.3359281254068023 2207 4 0.44678055136380834 2207 5 0.28721951239995036 2207 6 0.4226619998349008 2207 7 0.6452711794102015 2207 8 0.6971732875059047 2207 9 0.8095496308434562 2207 10 0.6265673537506444 2207 11 0.44153255425096205 2207 12 0.3592529603443708 2207 13 0.1687670636988301 2207 14 -4.693221602618756 2207 15 -1.7750881060517898 2207 16 -0.6293114711136027 2207 17 -0.44806261359718036 2207 18 0.1865397609345365 2207 19 0.5399860827383876 2207 20 0.3760359542620151 2207 21 0.5143312594633471 2207 22 0.45781739308998226 2207 23 0.5803291672159929 2207 24 0.08863840468209361 2207 25 0.2890266504225991 2208 1 0.5139084508538829 2208 2 0.7316556522904811 2208 3 0.7677630270753824 2208 4 0.7132130073060097 2208 5 0.7375453732423694 2208 6 0.7996828259334379 2208 7 0.7859135956017553 2208 8 1.0049875417683936 2208 9 1.2048469851838277 2208 10 1.0195787934385692 2208 11 0.9097296823599592 2208 12 0.9341031789897443 2208 13 0.7329887906770054 2208 14 1.2492387563170482 2208 15 0.8881892482211455 2208 16 0.9499723430597413 2208 17 0.6927079217046952 2208 18 0.878398465785196 2208 19 0.4907438398447894 2208 20 0.685318858115366 2208 21 0.9262598229446866 2208 22 0.7968549488470771 2208 23 1.096278984983421 2208 24 1.1284322003455998 2208 25 1.0595282367394816 2209 1 -0.2824447435171571 2209 2 -0.0771007269735513 2209 3 0.24256112449903 2209 4 0.5836691527334708 2209 5 0.5995335668732551 2209 6 0.6019070531708763 2209 7 0.6949350253740931 2209 8 0.6535139560576724 2209 9 0.8788060574199719 2209 10 0.5727493186431545 2209 11 0.6716142537678564 2209 12 0.5055017038969254 2209 13 0.5756049202429594 2209 14 0.8324704434167813 2209 15 0.6592069095472866 2209 16 0.7409066377286564 2209 17 1.1170385804191767 2209 18 0.6579477124643631 2209 19 0.9374909259302697 2209 20 0.9426007139110126 2209 21 0.9127487443425082 2209 22 0.8577227899882516 2209 23 0.872178344829841 2209 24 1.027867737208517 2209 25 1.007258963642343 2210 1 -0.40464786384403695 2210 2 -0.7644749001113134 2210 3 0.23924327589362093 2210 4 0.3600277959585938 2210 5 0.5972176712513644 2210 6 0.37059720584498734 2210 7 0.491815415712594 2210 8 0.5680531760261616 2210 9 0.9036655365622082 2210 10 0.9133179430986971 2210 11 0.4798869284228928 2210 12 0.44275807404432044 2210 13 0.5333772997629538 2210 14 0.7249545050487285 2210 15 0.4302129578432812 2210 16 0.44042473922498715 2210 17 0.6695600664653512 2210 18 0.6192688846561154 2210 19 0.5327449536449933 2210 20 0.3996784681879575 2210 21 0.6287979942404789 2210 22 0.5122713481298504 2210 23 0.7779775511568445 2210 24 0.49543910215250264 2210 25 0.6719855567081368 2211 1 -1.192743623183663 2211 2 -0.08880690198087038 2211 3 0.3317521066573299 2211 4 0.5181247900522266 2211 5 0.7288379033396666 2211 6 0.4038537521243981 2211 7 0.6661498037632043 2211 8 0.37641405144402285 2211 9 0.6759162961631802 2211 10 0.6372799732766907 2211 11 0.4033253623003006 2211 12 0.3596386763618682 2211 13 0.433050127826867 2211 14 0.7072588893344758 2211 15 0.4468624414812794 2211 16 0.6194974010894967 2211 17 0.5513077509228239 2211 18 0.6494665740753334 2211 19 0.6388550164356417 2211 20 0.6771405714664169 2211 21 0.739903129377872 2211 22 0.6811682734408748 2211 23 0.8510386275441846 2211 24 0.6134406793045528 2211 25 0.6909280467145891 2212 1 0.67228195563602 2212 2 -0.03528682826919294 2212 3 0.30602437450123077 2212 4 0.20057359363357108 2212 5 0.1313041110663633 2212 6 0.2644460825115402 2212 7 0.42707625651296865 2212 8 0.4428553845829484 2212 9 0.7142674627993415 2212 10 0.4868605364760725 2212 11 0.25473243005858137 2212 12 0.6601533550987213 2212 13 0.41768111961496857 2212 14 0.35904490831460834 2212 15 0.4145336985817636 2212 16 -0.21218031508953317 2212 17 0.40491282883817803 2212 18 0.25251400580051353 2212 19 0.24719737241711925 2212 20 0.4181763507905458 2212 21 0.42768563650550107 2212 22 0.4451815576744522 2212 23 0.9485980141488852 2212 24 0.7006909794562546 2212 25 0.12713301232511043 2213 1 0.5491746497960754 2213 2 0.20123718931120038 2213 3 -0.20654068559534977 2213 4 -0.8903421221651371 2213 5 -1.741450971475345 2213 6 -0.1549854695705477 2213 7 0.20283956893422328 2213 8 0.06178216424176952 2213 9 -0.6286733345370408 2213 10 -0.35816202200918923 2213 11 -0.25255332910813166 2213 12 -0.6079185340813069 2213 13 -0.5374998826748865 2213 14 -7.398352590657062 2213 15 -3.438644246247918 2213 16 -1.8298990093208403 2213 17 -1.1214059437729418 2213 18 -4.668958821993872 2213 19 -2.712606257021303 2213 20 -4.966316269524079 2213 21 -1.7944231365266603 2213 22 -1.3113123063527823 2213 23 -4.055994653776608 2213 24 -1.9797461337053974 2213 25 -1.5638011648326824 2214 1 0.48671985777388116 2214 2 0.27260926961464094 2214 3 -1.5069749636327394 2214 4 -1.6399112684415016 2214 5 -0.17818607125421693 2214 6 -0.30811233711152397 2214 7 -0.6874491710778017 2214 8 -0.7201969390008216 2214 9 -5.563886256489894 2214 10 -1.5666267379740866 2214 11 -0.7273320856037699 2214 12 -1.1817293824949076 2214 13 -1.2220793065833533 2214 14 -2.5774132404135592 2214 15 -1.9500631599054898 2214 16 -1.260219853447019 2214 17 -1.6632684139509166 2214 18 -0.8644608686076936 2214 19 -4.1106325388639 2214 20 -2.192269378003523 2214 21 -3.8936240987119177 2214 22 -6.141055426531182 2214 23 -6.0673952731552205 2214 24 -5.553205169062139 2214 25 -3.3925620229563265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24095.0 3 16.0 4 486.0 5 2866.0 6 3550.0 7 2741.0 8 2086.0 9 1732.0 10 1550.0 11 1391.0 12 1276.0 13 1276.0 14 1328.0 15 1277.0 16 1310.0 17 1492.0 18 1619.0 19 1971.0 20 2535.0 21 3273.0 22 4293.0 23 5141.0 24 6219.0 25 7073.0 26 8385.0 27 9327.0 28 10769.0 29 12961.0 30 15933.0 31 20164.0 32 25749.0 33 33270.0 34 43377.0 35 56784.0 36 76720.0 37 113200.0 38 182735.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.23419536686594 20.073287598699626 9.313124601973776 30.379392432460662 2 12.53410735152972 21.797098591630977 41.23546178671778 24.433332270121525 3 17.716681550898272 26.235197921620024 29.361176511522714 26.686944015958993 4 11.678095597083086 18.51646200907549 38.44991808863824 31.355524305203186 5 12.34544993295401 39.687601928025224 35.658898996122204 12.308049142898561 6 30.71071898315738 39.043712825765596 18.235162315365542 12.010405875711475 7 27.120644770675767 32.02364894786284 22.74643153231766 18.10927474914373 8 23.756943788894294 40.143777275928926 20.273612512979895 15.825666422196887 9 26.39014960619629 15.444250959637843 19.921253781546778 38.24434565261909 10 14.71754729288976 30.2944118286584 34.48691744582156 20.501123432630283 11 33.69118431427114 22.552148555261812 25.66062566671305 18.096041463754002 12 24.09721326420725 24.952496351169003 32.2632028257243 18.68708755889945 13 29.69462440395959 23.021290780758584 25.446468687044366 21.83761612823746 14 20.03240461381359 24.045848031934643 27.42106270887486 28.500684645376907 15 21.797508533165207 33.81379945068881 22.914103093680023 21.47458892246596 16 20.58961221546645 28.571407865844378 30.180956453050605 20.658023465638564 17 20.491094011122886 30.26470839753076 28.66178983634548 20.58240775500088 18 20.465751995106807 27.999617356921924 31.778507634019288 19.756123013951978 19 23.58051513803154 26.962494438010815 27.224686970523994 22.232303453433648 20 23.612886581502064 28.495647561155895 28.24824119729027 19.64322466005177 21 21.858631534704408 27.259590996710003 27.086250126817106 23.795527341768484 22 22.932011536733484 29.988115425308347 26.681015116599273 20.3988579213589 23 21.04746734998239 29.68669606895382 28.185614136287285 21.080222444776506 24 23.409525383098032 27.654800881781306 28.7360120121687 20.199661722951966 25 21.83152890705393 28.7470469730568 28.438772700262334 20.98265141962694 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 169.0 1 169.0 2 174.5 3 180.0 4 180.0 5 180.0 6 292.5 7 405.0 8 405.0 9 405.0 10 691.0 11 977.0 12 977.0 13 977.0 14 1760.0 15 2543.0 16 2543.0 17 2543.0 18 4777.5 19 7012.0 20 7012.0 21 7012.0 22 12185.0 23 17358.0 24 17358.0 25 17358.0 26 26615.0 27 35872.0 28 35872.0 29 35872.0 30 48973.0 31 62074.0 32 62074.0 33 62074.0 34 69436.5 35 76799.0 36 76799.0 37 76799.0 38 81086.5 39 85374.0 40 85374.0 41 85374.0 42 87777.5 43 90181.0 44 90181.0 45 90181.0 46 92089.5 47 93998.0 48 93998.0 49 93998.0 50 87346.0 51 80694.0 52 80694.0 53 80694.0 54 68050.0 55 55406.0 56 55406.0 57 55406.0 58 46365.0 59 37324.0 60 37324.0 61 37324.0 62 30069.5 63 22815.0 64 22815.0 65 22815.0 66 17399.5 67 11984.0 68 11984.0 69 11984.0 70 8715.0 71 5446.0 72 5446.0 73 5446.0 74 3809.0 75 2172.0 76 2172.0 77 2172.0 78 1498.5 79 825.0 80 825.0 81 825.0 82 545.0 83 265.0 84 265.0 85 265.0 86 170.0 87 75.0 88 75.0 89 75.0 90 48.0 91 21.0 92 21.0 93 21.0 94 10.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09145325159064886 2 0.0342043856979289 3 0.02927663521602389 4 0.028986767540617707 5 0.02101540646694784 6 0.386393611316434 7 0.17812368653709582 8 0.20363204197283938 9 0.2071104540777135 10 0.017392060524370624 11 0.002608809078655594 12 0.003333478267171036 13 0.0 14 0.08145281678913577 15 0.002174007565546328 16 0.0036233459425772134 17 0.0059422873458266304 18 0.004637882806498833 19 0.0027537429163586825 20 0.0017392060524370626 21 0.0 22 0.0 23 1.4493383770308854E-4 24 4.3480151310926565E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 689970.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.14124325679943 #Duplication Level Percentage of deduplicated Percentage of total 1 91.94456975402471 72.76607561045033 2 5.872910105359846 9.295788145471985 3 1.0177061634109448 2.41627593127349 4 0.3758081999443507 1.1896771267878312 5 0.19213209058755426 0.7602786259313528 6 0.11648627112771423 0.5531320991637554 7 0.08557282975923193 0.47406380953036426 8 0.058140474945963715 0.36810475766114936 9 0.044643272885664616 0.3179811707301655 >10 0.24169032463462853 3.5393555308147224 >50 0.025354167183103696 1.4295970191017668 >100 0.021128160337422555 3.538763985583414 >500 0.002020954466048863 0.9762207820390821 >1k 0.0018372313327716934 2.374685405460573 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2284 0.3310288853138542 No Hit GTCCTACAGTGGACATTTCTAAATT 2199 0.3187095091090917 No Hit CTTTAGGACGTGAAATATGGCGAGG 2155 0.3123324202501558 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2070 0.30001304404539325 No Hit GTCCTACAGTGTGCATTTCTCATTT 1639 0.23754655999536212 No Hit CTGTAGGACCTGGAATATGGCGAGA 1346 0.19508094554835717 No Hit GTATCAACGCAGAGTACTTTTTTTT 1187 0.1720364653535661 No Hit CTGAAGGACCTGGAATATGGCGAGA 1166 0.16899285476180123 No Hit CCCATGTACTCTGCGTTGATACCAC 1146 0.16609417800773946 No Hit GTACATGGGAAGCAGTGGTATCAAC 1140 0.16522457498152093 No Hit GTCCTTCAGTGTGCATTTCTCATTT 705 0.10217835558067742 No Hit GAATATGGCAAGAAAACTGAAAATC 703 0.10188848790527125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 1.4493383770308854E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 515 0.0 14.624377 4 TGTAGGA 1140 0.0 13.718023 2 GTAGGAC 1150 0.0 13.429958 3 TCTATAC 50 0.00145871 13.347058 3 GGACCTG 820 0.0 13.253079 6 CTGTAGG 1145 0.0 12.98524 1 AGGACCT 855 0.0 12.934492 5 GGCGAGG 625 0.0 12.584479 19 ACAGGTC 70 1.08733286E-4 12.216528 8 TGTCGAG 70 1.1149369E-4 12.183768 18 AGAACCG 55 0.0029854095 12.13369 5 TAGGACA 315 0.0 12.106174 4 GCCGGTT 55 0.0031065247 12.069448 11 CTAGGAC 95 9.93643E-7 12.042459 3 ACTGTTC 245 0.0 12.0226145 8 AGGACGT 1285 0.0 11.944839 5 GGACGTG 1255 0.0 11.849683 6 GTATAAG 65 7.798492E-4 11.730256 1 GACGTGG 540 0.0 11.611555 7 TAGGACG 1330 0.0 11.540689 4 >>END_MODULE