FastQCFastQC Report
Thu 26 May 2016
SRR1926695_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926695_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673429
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9300.13809919085753658No Hit
GTCCTAAAGTGTGTATTTCTCATTT8530.12666517182954698No Hit
CTGTAGGACGTGGAATATGGCAAGA7100.1054305650632806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAC300.00739479218.3378563
TAGACTC350.001137637917.9316445
CTATAGG807.8412086E-716.804231
GTTCTAG851.476641E-615.8157451
CCAACGT501.3351668E-515.26830119
ATTAGTA656.5216026E-414.8435612
GATCAGA657.8808935E-414.477491
ATTTAGA1601.8189894E-1114.2835961
TCCAACG1250.014.25876518
TAGGACC2850.014.001614
GTTTAGG600.00775111913.4433831
GTGCTAT600.00775111913.4433831
GTGTAGG700.001379724213.4433831
TGTAGGA6650.013.2651372
CTGTAGG6400.013.2333321
GTCCTAC6700.013.04208851
TTTAGAA1808.913048E-1113.0175672
TAGAAAT2250.012.9420274
GTCCTGA1254.2792817E-712.9056491
AGGACCT4550.012.8740015
Produced by FastQC (version 0.11.5)