##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926695_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 673429 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.303280672498513 27.0 2.0 30.0 2.0 31.0 2 15.858913114819826 16.0 2.0 30.0 2.0 31.0 3 19.4074119172177 20.0 12.0 31.0 2.0 31.0 4 21.481473176830818 27.0 2.0 35.0 2.0 37.0 5 21.832399555112715 28.0 2.0 35.0 2.0 37.0 6 22.66603012344286 30.0 2.0 35.0 2.0 37.0 7 22.525284773895986 30.0 2.0 35.0 2.0 37.0 8 22.659693894976307 30.0 2.0 35.0 2.0 37.0 9 25.273754174530648 31.0 17.0 37.0 2.0 39.0 10 25.88564644528228 31.0 17.0 37.0 2.0 39.0 11 23.065111541083024 29.0 7.0 37.0 2.0 39.0 12 24.100424840629078 30.0 2.0 37.0 2.0 39.0 13 20.835938161261247 27.0 2.0 35.0 2.0 39.0 14 24.11545834824458 30.0 8.0 37.0 2.0 40.0 15 25.184441121484223 31.0 8.0 38.0 2.0 40.0 16 25.550702449701454 31.0 7.0 38.0 2.0 40.0 17 24.88000368264509 30.0 2.0 38.0 2.0 40.0 18 24.96374821993113 30.0 2.0 38.0 2.0 40.0 19 25.342932959525058 31.0 2.0 38.0 2.0 40.0 20 25.555313180751053 31.0 2.0 38.0 2.0 40.0 21 23.45533530632034 30.0 2.0 37.0 2.0 40.0 22 24.36676769191704 30.0 2.0 37.0 2.0 40.0 23 24.338160370284022 31.0 2.0 38.0 2.0 40.0 24 23.41658734625328 30.0 2.0 38.0 2.0 40.0 25 23.21978560471854 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.831686999058412 1101 2 10.695746611551156 1101 3 7.509908714864405 1101 4 8.875735778254345 1101 5 8.482702041782783 1101 6 8.275150497401121 1101 7 7.618538790572739 1101 8 5.991353796784544 1101 9 5.97432517372631 1101 10 5.796998551765899 1101 11 8.40069949687646 1101 12 6.400887857743985 1101 13 10.642482416105029 1101 14 8.02723125463482 1101 15 7.151900215782433 1101 16 6.787337281147856 1101 17 3.0633679769102393 1101 18 5.161117737075379 1101 19 5.986882317250142 1101 20 3.845513177676459 1101 21 1.4219876298468535 1101 22 5.0210712336045695 1101 23 6.151098476579524 1101 24 7.3982738579733756 1101 25 7.9491735212720265 1102 1 5.337448507802964 1102 2 10.192839690128432 1102 3 6.702035128678073 1102 4 7.871363502006414 1102 5 7.8468871205376765 1102 6 6.827736753514696 1102 7 6.7462926743973775 1102 8 6.754315209107482 1102 9 5.745916955052234 1102 10 4.833795768226082 1102 11 7.42557246774064 1102 12 4.802878773812772 1102 13 9.090877820853795 1102 14 6.962727096452621 1102 15 4.68308816815523 1102 16 5.23409129818657 1102 17 5.869251281380524 1102 18 5.941687195528445 1102 19 4.563367812774782 1102 20 4.896104394428448 1102 21 4.006383504016643 1102 22 4.746957900144366 1102 23 5.300252250026787 1102 24 6.905462660378841 1102 25 6.387279724607453 1103 1 4.549192280044959 1103 2 8.712412643865084 1103 3 6.0685831713826985 1103 4 7.632929338305349 1103 5 6.708986764665788 1103 6 6.655138888746013 1103 7 6.8059012153226455 1103 8 6.162678198431326 1103 9 5.4772336810308815 1103 10 4.516180109863093 1103 11 5.209380296922134 1103 12 4.145405464204231 1103 13 7.8070699916723 1103 14 6.365752007484652 1103 15 5.242696709080498 1103 16 5.027685604236392 1103 17 2.749144519814685 1103 18 2.05378683965656 1103 19 3.837389165087952 1103 20 4.222616849944462 1103 21 6.452113041383193 1103 22 2.3145735585073552 1103 23 4.289576093443156 1103 24 5.59407476002297 1103 25 1.3063188705149251 1104 1 5.463885682727685 1104 2 9.998245583167774 1104 3 6.842470760661747 1104 4 7.46047456887549 1104 5 7.423072945388981 1104 6 7.4632418178453825 1104 7 7.3209417797404335 1104 8 7.0929752145824345 1104 9 5.788976208965579 1104 10 4.931579656935238 1104 11 7.90291052114631 1104 12 4.762964506585337 1104 13 8.899441143651995 1104 14 6.789880370225113 1104 15 6.043165812930383 1104 16 5.65330464711522 1104 17 5.997757117181731 1104 18 3.6479715695174484 1104 19 4.449003529371353 1104 20 3.391413355929579 1104 21 2.995825556625956 1104 22 5.150321884914046 1104 23 5.512232440654664 1104 24 6.299394795859019 1104 25 5.530244411166446 1105 1 3.063578524608026 1105 2 8.832022049756743 1105 3 6.009258833699821 1105 4 4.819558193584115 1105 5 2.0583621353656696 1105 6 4.484876433229999 1105 7 5.320735388909043 1105 8 2.602924466719184 1105 9 3.4326910011178704 1105 10 3.269568924612006 1105 11 6.37149662254491 1105 12 3.7225604661812994 1105 13 4.6823665515774024 1105 14 3.538047134016878 1105 15 4.046716949294019 1105 16 4.3795879124609876 1105 17 4.725861478098594 1105 18 2.2829936654770435 1105 19 2.2321474687652945 1105 20 1.267360325626548 1105 21 2.3316355799359805 1105 22 3.6759750511922107 1105 23 1.267185820608045 1105 24 4.0529641431783645 1105 25 4.75860979578183 1106 1 6.381343225185226 1106 2 11.300584910507103 1106 3 7.908762801953788 1106 4 9.35055115895208 1106 5 8.723980371296406 1106 6 7.993128597732163 1106 7 7.895442279240932 1106 8 7.786922933530153 1106 9 6.76864653863591 1106 10 5.856254981610565 1106 11 8.114374057609844 1106 12 5.75960952823036 1106 13 4.68572152993238 1106 14 6.082820763165508 1106 15 6.312862782627352 1106 16 6.095995289805863 1106 17 6.385802405227022 1106 18 6.699594946140824 1106 19 6.512856343224168 1106 20 6.137084351600574 1106 21 5.854200515000915 1106 22 6.347732807324967 1106 23 6.7131814916037165 1106 24 7.378207649671872 1106 25 6.3362883672104005 1107 1 4.074675833999006 1107 2 -13.437943228021036 1107 3 -5.225925382943602 1107 4 2.2616127612270702 1107 5 4.782749774835686 1107 6 5.055404675907823 1107 7 5.141326102530613 1107 8 5.5179269365006824 1107 9 3.919784939983515 1107 10 3.3816367128500353 1107 11 5.861317490745748 1107 12 3.208485617273812 1107 13 4.388817039722451 1107 14 4.14283996293366 1107 15 3.9301570643567807 1107 16 3.90409904100585 1107 17 4.599492202180414 1107 18 -0.8186792482839991 1107 19 1.9817726431028113 1107 20 2.443885778904093 1107 21 -0.5743093294085106 1107 22 2.484022180434426 1107 23 -0.5566984192803801 1107 24 2.8505147010584224 1107 25 3.7866171179575616 1108 1 5.384881250945476 1108 2 10.473990928357564 1108 3 0.9149944304076385 1108 4 6.348146156547077 1108 5 7.582288318493244 1108 6 7.3726593550407 1108 7 7.4347400185386725 1108 8 7.029988509929062 1108 9 5.741828089595238 1108 10 4.830120622142871 1108 11 7.101942867400876 1108 12 2.7815110834652508 1108 13 3.2472842470506507 1108 14 4.885770899448975 1108 15 5.097224922545049 1108 16 5.060625721102962 1108 17 0.3413900274813102 1108 18 3.7181081202095534 1108 19 4.924817221851715 1108 20 5.45185106636729 1108 21 5.123250806273429 1108 22 1.924933384525545 1108 23 4.94095125270681 1108 24 5.4256236081989435 1108 25 6.071032635288002 1109 1 3.8556773466289265 1109 2 -13.437943228021036 1109 3 -5.304088999681447 1109 4 2.631199874630994 1109 5 1.6581675531108537 1109 6 2.911456459114383 1109 7 4.089646866715579 1109 8 4.417600222878711 1109 9 3.869044328938788 1109 10 3.0024871790938406 1109 11 1.1498009258235982 1109 12 1.7572287626054184 1109 13 2.9487240436682853 1109 14 3.8401739075764603 1109 15 4.0561472232370726 1109 16 3.8855279889157828 1109 17 4.248905020184683 1109 18 4.61121378459314 1109 19 3.121860224014071 1109 20 2.035758010412284 1109 21 0.9801652031744048 1109 22 3.3110516857430703 1109 23 3.8253897320259327 1109 24 4.86331609888731 1109 25 5.211451685341796 1110 1 3.5189302758157552 1110 2 9.32164756481784 1110 3 6.644799825458843 1110 4 7.811099647122049 1110 5 8.520018944828585 1110 6 4.265114071763673 1110 7 6.918779044777185 1110 8 7.700646209401764 1110 9 6.03132742126564 1110 10 5.59894383580837 1110 11 3.611193180515926 1110 12 6.36752352386814 1110 13 10.593811564365126 1110 14 8.304027348566464 1110 15 7.5239454765745 1110 16 7.008022393137313 1110 17 7.689733938314564 1110 18 7.63356600492007 1110 19 6.352804970896582 1110 20 6.769251843103106 1110 21 5.146586560328135 1110 22 7.0230862688676865 1110 23 7.407744217879998 1110 24 8.27512420285441 1110 25 8.697681620163756 1111 1 5.1858550831322 1111 2 9.51540706884071 1111 3 7.544749715803299 1111 4 8.20265562785044 1111 5 8.401959728665076 1111 6 7.6963109116015005 1111 7 7.512489523769094 1111 8 7.800264638474911 1111 9 6.1623413933994655 1111 10 5.6152869589631536 1111 11 7.508231938490628 1111 12 6.770164367792834 1111 13 8.460038307302593 1111 14 6.175809538597029 1111 15 5.903023936147285 1111 16 5.783366273360052 1111 17 6.826019185901817 1111 18 6.500610554790018 1111 19 5.33899119225978 1111 20 5.1357074418267175 1111 21 7.838049914117487 1111 22 7.21110810886821 1111 23 7.186715082694558 1111 24 7.046099215273362 1111 25 7.722959880599472 1112 1 -1.6544253540616118 1112 2 7.672424664955551 1112 3 5.768398094197774 1112 4 -0.11158380753071384 1112 5 3.625452087985515 1112 6 2.554378941591203 1112 7 5.442977352862965 1112 8 5.758108993846648 1112 9 4.416598586587956 1112 10 3.272184704062024 1112 11 0.22964357070544494 1112 12 3.992859611480444 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2204 9 3.649116069105439 2204 10 3.4324537828093646 2204 11 4.776162269398057 2204 12 4.869607863228694 2204 13 3.165175409386258 2204 14 3.9183768737216162 2204 15 4.356102875867446 2204 16 4.2616962555068305 2204 17 -0.7286414842168689 2204 18 2.7913282128740917 2204 19 3.6701573755252 2204 20 3.892287481803706 2204 21 5.144426955227356 2204 22 4.8435037030958625 2204 23 3.4580366364588606 2204 24 5.070373816838039 2204 25 5.268006648928683 2205 1 5.607317251159252 2205 2 10.020065429987623 2205 3 6.7633082564992435 2205 4 7.476958085359005 2205 5 7.573331020647057 2205 6 7.18100738561095 2205 7 7.127476911275565 2205 8 6.963546310153529 2205 9 5.747867317856688 2205 10 5.032012557368141 2205 11 7.250304793540582 2205 12 6.48774805636889 2205 13 9.408665252876101 2205 14 6.753816434161177 2205 15 5.647927717692287 2205 16 5.19383078764136 2205 17 6.0386162580408715 2205 18 5.814746461292337 2205 19 4.679955910323734 2205 20 5.006348420864644 2205 21 -1.0012107404103396 2205 22 3.506174365766526 2205 23 4.809285387707611 2205 24 6.409376480840702 2205 25 6.747543778465811 2206 1 3.9291097686928254 2206 2 8.323555920190207 2206 3 5.5062479970539755 2206 4 1.5045471913126782 2206 5 5.321666898284466 2206 6 5.5994952577615145 2206 7 5.992030699338724 2206 8 5.724560196891435 2206 9 4.345703553171628 2206 10 3.3697088190848454 2206 11 2.859717981147977 2206 12 4.177533966086656 2206 13 7.561483530566612 2206 14 0.20178873443330758 2206 15 2.0900916298732355 2206 16 2.8230607853551746 2206 17 4.059222908630488 2206 18 -1.2184718252479243 2206 19 0.655717173478525 2206 20 2.1789694965521598 2206 21 2.9155012812420544 2206 22 -2.0118828183894664 2206 23 -2.8174961782562384 2206 24 0.7288407757734809 2206 25 2.260260136497333 2207 1 1.3657922525527084 2207 2 -13.437943228021036 2207 3 -6.006463030868611 2207 4 -4.254874031022467 2207 5 1.3903386196289027 2207 6 1.8063088366634759 2207 7 2.906779439934315 2207 8 3.0100022862232407 2207 9 0.729507137264747 2207 10 0.45729171311939965 2207 11 -1.3425792894722584 2207 12 -1.997813878120084 2207 13 0.15915482053305396 2207 14 -5.2897708798124015 2207 15 -1.5105993060707874 2207 16 -0.46909398429628624 2207 17 0.3470383080938255 2207 18 0.5505954477851027 2207 19 0.03353337720592364 2207 20 -0.22737322229905743 2207 21 -3.8093336618379823 2207 22 -4.440064231115848 2207 23 -1.6184722623676358 2207 24 0.09717909310683481 2207 25 0.5511258902024316 2208 1 4.54308091513224 2208 2 6.055106649329742 2208 3 6.1795567123225155 2208 4 7.79177899625514 2208 5 3.396335271778863 2208 6 5.773025452036215 2208 7 6.084659766577797 2208 8 6.478856402078502 2208 9 4.436788535641355 2208 10 4.100092256355442 2208 11 5.793863497033872 2208 12 5.807926596825435 2208 13 8.58755188818138 2208 14 3.213752083140161 2208 15 4.684026587478812 2208 16 5.099399026979022 2208 17 5.960381982096049 2208 18 1.1686268137009002 2208 19 3.461510033496829 2208 20 4.265103239226985 2208 21 6.753531873203901 2208 22 5.72898535145568 2208 23 2.026214166206298 2208 24 3.2196563617665355 2208 25 4.565513924972343 2209 1 2.78886249316961 2209 2 4.4096019916172065 2209 3 4.549122098127036 2209 4 0.17433048040581767 2209 5 4.498816215899694 2209 6 5.008643659758853 2209 7 5.205627184774677 2209 8 3.720542629940546 2209 9 3.3612749266131345 2209 10 2.282374426334659 2209 11 -1.432921712941738 2209 12 2.243985961444004 2209 13 1.3685207290106476 2209 14 2.7754636930177377 2209 15 3.0925354785791193 2209 16 2.590054374562623 2209 17 -0.028061594683492785 2209 18 2.1731947313685147 2209 19 2.7719030673871714 2209 20 2.7872515831631546 2209 21 4.988558491219354 2209 22 3.8108937635091813 2209 23 -0.7609149545857541 2209 24 -3.587889646657981 2209 25 -0.4811350362595128 2210 1 -1.7243060101265293 2210 2 -13.437943228021036 2210 3 -7.178457023809555 2210 4 -1.4393875583401012 2210 5 -0.016686895125744172 2210 6 -0.6997927497184904 2210 7 -0.48164706125870893 2210 8 -20.22433247772526 2210 9 -12.264983596770426 2210 10 -6.1313139326013335 2210 11 -9.240584305519032 2210 12 -3.7263984838877775 2210 13 -4.247399582229583 2210 14 -3.0962193204926933 2210 15 -4.673046039160688 2210 16 -4.1111502585475 2210 17 -3.502683827308946 2210 18 -3.765418548357573 2210 19 -9.148162625965316 2210 20 -6.099665281241421 2210 21 -4.410408529004222 2210 22 -8.689591975648128 2210 23 -6.570662018349921 2210 24 -9.54119290241074 2210 25 -7.169065603862931 2211 1 0.21388568272768538 2211 2 4.863630198552391 2211 3 0.9429952361862206 2211 4 1.7445654779663968 2211 5 1.4886323859484207 2211 6 0.6870180416216094 2211 7 -20.083778499979847 2211 8 -12.838842967235749 2211 9 -8.426058756069384 2211 10 -6.749364399008815 2211 11 -9.127683884448096 2211 12 -4.468678850058019 2211 13 -5.851432982222132 2211 14 -4.28791683257209 2211 15 -4.839701319936751 2211 16 -11.099317730507156 2211 17 -7.226019106594492 2211 18 -5.414091367545492 2211 19 -8.40589157552375 2211 20 -6.339355874839651 2211 21 -4.026027590227191 2211 22 -5.481671122078961 2211 23 -7.331299027876806 2211 24 -21.061619190154968 2211 25 -20.83514020421817 2212 1 -1.0060352022644388 2212 2 -13.437943228021036 2212 3 -7.622348591846832 2212 4 -19.081483473082553 2212 5 -19.345283697967663 2212 6 -20.17137356677 2212 7 -9.675051874868538 2212 8 -9.086309325454556 2212 9 -4.896436552805152 2212 10 -5.4072044440928515 2212 11 -5.392730058220895 2212 12 -2.691793446948214 2212 13 -5.843716855961928 2212 14 -3.6706773267528874 2212 15 -3.6281286094771303 2212 16 -3.69790247167872 2212 17 -3.9229130407992017 2212 18 -2.9767450984984194 2212 19 -3.5260430180260407 2212 20 -3.658554263272233 2212 21 -1.5909861178972058 2212 22 -2.974522438171597 2212 23 -3.104623321570646 2212 24 -2.2272469728798114 2212 25 -2.712255075099737 2213 1 -17.930345086503085 2213 2 -13.437943228021036 2213 3 -8.694116489297597 2213 4 -5.827529984710461 2213 5 -19.345283697967663 2213 6 -10.75369914816535 2213 7 -20.083778499979847 2213 8 -10.598285966097352 2213 9 -8.400861162964816 2213 10 -7.525756495284632 2213 11 -6.566737389082995 2213 12 -5.304403358108107 2213 13 -18.359300115089265 2213 14 -12.06457076003997 2213 15 -7.265121713496679 2213 16 -6.3685330500713135 2213 17 -5.137656771255411 2213 18 -5.128614054161201 2213 19 -6.03105989557579 2213 20 -6.03338579840446 2213 21 -4.275903179753943 2213 22 -5.185040775681173 2213 23 -4.9695971251283915 2213 24 -4.042084306434038 2213 25 -4.823047180962355 2214 1 -17.930345086503085 2214 2 -13.437943228021036 2214 3 -12.113065491407351 2214 4 -19.081483473082553 2214 5 -19.345283697967663 2214 6 -20.17137356677 2214 7 -16.008700293514778 2214 8 -14.57991120556676 2214 9 -13.626234492741473 2214 10 -13.568846923783312 2214 11 -13.7823175719285 2214 12 -11.92213597687281 2214 13 -18.359300115089265 2214 14 -16.182916905491872 2214 15 -22.82047055070598 2214 16 -17.613443203574235 2214 17 -22.530214910790296 2214 18 -17.16562450593994 2214 19 -14.376548655669882 2214 20 -13.226394019177066 2214 21 -10.960878323435589 2214 22 -11.906534579789135 2214 23 -11.87849544459877 2214 24 -10.831170806421913 2214 25 -10.556725188576877 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 137357.0 3 9.0 4 713.0 5 2674.0 6 3345.0 7 3294.0 8 2850.0 9 2654.0 10 2452.0 11 2289.0 12 2181.0 13 2403.0 14 3391.0 15 6980.0 16 10098.0 17 10782.0 18 11294.0 19 12902.0 20 15527.0 21 17842.0 22 18432.0 23 19867.0 24 20828.0 25 21230.0 26 17716.0 27 14290.0 28 14982.0 29 17477.0 30 16786.0 31 19268.0 32 24591.0 33 30443.0 34 36338.0 35 40977.0 36 39774.0 37 40888.0 38 28505.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87691427823313 19.12142165627918 13.193145290487953 25.80851877499974 2 12.915915809806485 20.479370575833595 43.20483375145754 23.39987986290237 3 18.342857233034913 25.334204450588555 30.110672922298548 26.21226539407798 4 11.052886696096252 17.422141435125077 39.29126633774777 32.2337055310309 5 12.292639376500537 38.10730271333261 36.84086251678275 12.759195393384108 6 30.48098041554687 38.20143708204981 18.848895874704407 12.468686627698915 7 26.50063290098798 31.16125002054873 23.873354046456576 18.464763032006708 8 23.443078973718357 39.09213546814683 20.8899313258016 16.574854232333216 9 26.225306930634197 15.248049305123018 20.519719522111345 38.00692424213144 10 14.33071554807617 29.70492614105882 35.32954538600269 20.634812924862324 11 32.823237153688574 21.87463502555216 26.152687948030795 19.149439872728475 12 24.177315927454764 24.64294585687457 32.50691799818684 18.672820217483828 13 28.7474599836013 21.98388649246016 25.39410359701971 23.874549926918824 14 19.421148731610735 23.457763400131995 28.220962667959448 28.900125200297822 15 21.630339389836532 33.347475214693674 23.490481799092365 21.531703596377433 16 20.214297210917202 28.16736029676638 30.33853747518644 21.27980501712998 17 20.105841981229585 28.942544893262223 30.106776061214397 20.8448370642938 18 20.063311766694028 27.379019603109462 32.936151079136685 19.62151755105982 19 22.854997013916957 26.5022410704052 27.627903890353444 23.014858025324397 20 23.08452866487683 28.36840780796074 28.685127856920563 19.861935670241866 21 21.48053758522294 26.3353279624599 27.023023807967938 25.16111064434923 22 22.06511855528401 29.53822497862712 28.133958861361762 20.262697604727105 23 20.840171489053006 28.80350113572847 29.11939419886546 21.236933176353062 24 22.42812024415389 27.42116545844311 29.439362450702845 20.71135184670016 25 21.719164397083834 27.939654864835934 29.37662785580914 20.96455288227109 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 780.0 1 780.0 2 1571.0 3 2362.0 4 2362.0 5 2362.0 6 3428.5 7 4495.0 8 4495.0 9 4495.0 10 5223.0 11 5951.0 12 5951.0 13 5951.0 14 7440.5 15 8930.0 16 8930.0 17 8930.0 18 12897.5 19 16865.0 20 16865.0 21 16865.0 22 23485.0 23 30105.0 24 30105.0 25 30105.0 26 39272.0 27 48439.0 28 48439.0 29 48439.0 30 58575.5 31 68712.0 32 68712.0 33 68712.0 34 73978.0 35 79244.0 36 79244.0 37 79244.0 38 80953.0 39 82662.0 40 82662.0 41 82662.0 42 82338.0 43 82014.0 44 82014.0 45 82014.0 46 79744.0 47 77474.0 48 77474.0 49 77474.0 50 69693.5 51 61913.0 52 61913.0 53 61913.0 54 52703.0 55 43493.0 56 43493.0 57 43493.0 58 35909.5 59 28326.0 60 28326.0 61 28326.0 62 22524.5 63 16723.0 64 16723.0 65 16723.0 66 12761.0 67 8799.0 68 8799.0 69 8799.0 70 6238.5 71 3678.0 72 3678.0 73 3678.0 74 2648.0 75 1618.0 76 1618.0 77 1618.0 78 1096.0 79 574.0 80 574.0 81 574.0 82 387.0 83 200.0 84 200.0 85 200.0 86 127.5 87 55.0 88 55.0 89 55.0 90 33.0 91 11.0 92 11.0 93 11.0 94 7.0 95 3.0 96 3.0 97 3.0 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.798485066725668 2 36.96217418614286 3 5.904111643543715 4 14.222583227036555 5 13.842587711547912 6 15.35202671699615 7 9.669764741346155 8 7.756571219831637 9 0.10691550259938316 10 1.5254763308381432 11 5.91346675002116 12 6.144968511899547 13 16.692034349575085 14 1.676494478259772 15 3.0473888115896406 16 3.1278724260463986 17 3.026302698576984 18 1.5854678072966861 19 1.5373558311269635 20 0.27590139420785265 21 4.559203717095641 22 1.6891164473166436 23 0.10884592139631646 24 9.256952106309647 25 0.11359772151184461 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 673429.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.36552015496152 #Duplication Level Percentage of deduplicated Percentage of total 1 96.8171942860198 88.45753315886166 2 2.2586932635010757 4.127333697805667 3 0.39882699176845215 1.0931710666428955 4 0.17034892528739592 0.6225607266688642 5 0.09482330808141479 0.4331790432836313 6 0.05286103465079423 0.28978055560795557 7 0.035377004154825184 0.22625668702908863 8 0.026716658473868136 0.19527851186139378 9 0.016926900865133147 0.1391881591938922 >10 0.11023202148801867 1.9181854017284565 >50 0.008747595335756489 0.5500815254992212 >100 0.008410918523051316 1.3666433936385514 >500 8.410918504416797E-4 0.5808080721787684 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 930 0.13809919085753658 No Hit GTCCTAAAGTGTGTATTTCTCATTT 853 0.12666517182954698 No Hit CTGTAGGACGTGGAATATGGCAAGA 710 0.1054305650632806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 1.484937536102544E-4 10 0.0 0.0 0.0 0.0 1.484937536102544E-4 11 0.0 0.0 0.0 0.0 1.484937536102544E-4 12 0.0 0.0 0.0 0.0 2.969875072205088E-4 13 0.0 0.0 0.0 0.0 2.969875072205088E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTAGAC 30 0.007394792 18.337856 3 TAGACTC 35 0.0011376379 17.931644 5 CTATAGG 80 7.8412086E-7 16.80423 1 GTTCTAG 85 1.476641E-6 15.815745 1 CCAACGT 50 1.3351668E-5 15.268301 19 ATTAGTA 65 6.5216026E-4 14.843561 2 GATCAGA 65 7.8808935E-4 14.47749 1 ATTTAGA 160 1.8189894E-11 14.283596 1 TCCAACG 125 0.0 14.258765 18 TAGGACC 285 0.0 14.00161 4 GTTTAGG 60 0.007751119 13.443383 1 GTGCTAT 60 0.007751119 13.443383 1 GTGTAGG 70 0.0013797242 13.443383 1 TGTAGGA 665 0.0 13.265137 2 CTGTAGG 640 0.0 13.233332 1 GTCCTAC 670 0.0 13.0420885 1 TTTAGAA 180 8.913048E-11 13.017567 2 TAGAAAT 225 0.0 12.942027 4 GTCCTGA 125 4.2792817E-7 12.905649 1 AGGACCT 455 0.0 12.874001 5 >>END_MODULE