FastQCFastQC Report
Thu 26 May 2016
SRR1926695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926695_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673429
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGGACGTGAAATATGGCGAGG20410.3030757511185292No Hit
GTCCTACAGTGGACATTTCTAAATT20090.2983239510030011No Hit
CTGTAGGACGTGGAATATGGCAAGA19990.2968390134668985No Hit
GTCCTAAAGTGTGTATTTCTCATTT18450.27397097541091936No Hit
GTCCTACAGTGTGCATTTCTCATTT15360.22808640554535073No Hit
CTGTAGGACCTGGAATATGGCGAGA12380.18383526696949493No Hit
GTATCAACGCAGAGTACTTTTTTTT11570.17180727292706433No Hit
CCCATGTACTCTGCGTTGATACCAC10260.152354591204121No Hit
GTACATGGGAAGCAGTGGTATCAAC9820.1458208660452698No Hit
CTGAAGGACCTGGAATATGGCGAGA9180.13631726581421352No Hit
GATATACACTGTTCTACAAATCCCG7240.10750947761382418No Hit
GGTATCAACGCAGAGTACTTTTTTT7100.1054305650632806No Hit
GAATATGGCAAGAAAACTGAAAATC6780.10067876494775248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACT350.002213038716.23619719
ATAAGAC704.20594E-714.9956453
GTGCGTT400.0053535314.21828611
TAGGACC4650.013.7486444
GCCGGTT903.7342033E-813.69168311
ATAGGAC1204.7293724E-1113.51883
CGGTTTC1052.0485459E-812.63847613
CCGGTTT1001.4800025E-712.32251512
TATAGGA1404.7293724E-1112.2712
CTATAGG1251.3133103E-912.2109791
TGTAGGA13000.012.1136792
TCCAACG1201.0428266E-811.83889318
TTACACA1052.5600275E-711.8138674
CTGTAGG13250.011.7357881
TAGGACT1001.8246028E-611.4503644
GTCTTAG1001.8295377E-611.4477951
GTAGGAC13650.011.3952913
TAGGACA3950.011.3537364
AGGACCT7850.011.3044985
GGCGAGG4800.011.24694819
Produced by FastQC (version 0.11.5)