FastQCFastQC Report
Thu 26 May 2016
SRR1926694_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926694_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697685
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10710.15350767180031102No Hit
GTCCTAAAGTGTGTATTTCTCATTT8800.12613142034012484No Hit
CTGTAGGACGTGGAATATGGCAAGA8400.12039817396102825No Hit
CTTTAGGACGTGAAATATGGCGAGG7290.10448841525903522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACC359.608491E-418.3886474
TAGGACC3400.017.6675244
TGTAGGA7450.016.5345732
CCTCTAT500.002423266616.0146641
CTGTAGG7350.015.6152051
GTATAGC801.562777E-515.0137461
CATTATG550.003864275314.9312182
GTATTAC902.8886952E-614.8283921
AGGATAG507.5202296E-414.5689385
GTAGGAC8250.014.3117823
GTCCTAC6850.014.0274421
GGACCTG5600.013.5601386
AGGACCT6200.013.4275935
CTGTTAG600.00813555313.3455521
GTCCTAA4750.013.2050731
AGGACGT7350.013.0256795
GGACGTG7250.012.91319756
TAGCCCT1356.9303496E-1012.7131394
TTAGGAC4400.012.6718893
TCCTAAA5000.012.5919952
Produced by FastQC (version 0.11.5)