FastQCFastQC Report
Thu 26 May 2016
SRR1926694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926694_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697685
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA23050.33037832259544064No Hit
GTCCTACAGTGGACATTTCTAAATT22870.32779836172484716No Hit
CTTTAGGACGTGAAATATGGCGAGG21360.30615535664375754No Hit
GTCCTAAAGTGTGTATTTCTCATTT20840.298702136350932No Hit
GTCCTACAGTGTGCATTTCTCATTT14320.2052502203716577No Hit
CTGAAGGACCTGGAATATGGCGAGA11920.17085074209707818No Hit
CTGTAGGACCTGGAATATGGCGAGA11540.16540415803693642No Hit
GTATCAACGCAGAGTACTTTTTTTT11470.16440083992059454No Hit
GTACATGGGAAGCAGTGGTATCAAC9050.12971469932706023No Hit
CCCATGTACTCTGCGTTGATACCAC8640.1238381217884862No Hit
GTCCTTCAGTGTGCATTTCTCATTT7170.10276844134530627No Hit
GAATATGGCAAGAAAACTGAAAATC7050.10104846743157728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG551.9053671E-413.8623821
TCCAACG1101.9281288E-1013.78091418
TAGGACC5700.013.7153264
CCAACGT500.001528900213.26412919
GAGGTGT655.2654468E-513.2006796
TAGGACT1301.2732926E-1113.2006794
CGAAATC655.5351542E-513.13060413
TGTAGGA12500.012.9697422
CTGTAGG12300.012.7846661
GCCTTAT603.9783117E-412.7071831
GGACCTG8400.012.5982676
AGGACCT8800.012.3506365
ACGAAAT701.1073662E-412.19270412
ACTGTTC3150.012.0664138
AGGTGTG1209.977157E-911.8744617
CACGTTT1201.03791535E-811.84297418
GTAGGAC13050.011.8376523
TGTCCAC2500.011.76002810
ATAGTAC657.76406E-411.7364763
TTATACT1302.4610927E-911.7339364
Produced by FastQC (version 0.11.5)