##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926694_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 697685 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.20486895948745 31.0 28.0 31.0 20.0 34.0 2 28.731051979044985 31.0 28.0 33.0 23.0 34.0 3 29.07921339859679 31.0 28.0 33.0 24.0 34.0 4 32.846786157076615 35.0 33.0 37.0 27.0 37.0 5 32.49589141231358 35.0 33.0 37.0 25.0 37.0 6 32.53256985602385 35.0 33.0 37.0 25.0 37.0 7 32.36901466994417 35.0 33.0 37.0 23.0 37.0 8 32.32711897202893 35.0 33.0 37.0 23.0 37.0 9 33.5651662283122 37.0 33.0 39.0 20.0 39.0 10 33.4247117252055 37.0 33.0 39.0 19.0 39.0 11 33.658566545074066 37.0 33.0 39.0 23.0 39.0 12 33.471893476282276 37.0 33.0 39.0 21.0 39.0 13 33.60630657101701 37.0 33.0 39.0 23.0 39.0 14 34.23239570866509 38.0 33.0 40.0 18.0 41.0 15 34.35758830991063 38.0 33.0 40.0 19.0 41.0 16 34.35186223008951 38.0 34.0 40.0 19.0 41.0 17 34.26224872256104 38.0 33.0 40.0 19.0 41.0 18 34.24697535420713 38.0 33.0 40.0 18.0 41.0 19 34.28265191311265 39.0 34.0 40.0 18.0 41.0 20 34.26514544529408 39.0 34.0 40.0 18.0 41.0 21 34.18755885535736 39.0 34.0 40.0 18.0 41.0 22 34.10701964353541 39.0 34.0 40.0 18.0 41.0 23 33.76127765395558 39.0 33.0 40.0 10.0 41.0 24 33.85648537663845 39.0 34.0 40.0 10.0 41.0 25 33.64397113310448 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2966799352185454 1101 2 0.4658554866066211 1101 3 0.35028446614268205 1101 4 0.09345529509315043 1101 5 0.08295384096556546 1101 6 -0.21168086749049309 1101 7 -0.03336006466665964 1101 8 -0.015603836261114168 1101 9 0.24235418493974237 1101 10 0.22991909823296197 1101 11 -0.041609135235596284 1101 12 0.19144423835567181 1101 13 0.03760222407615288 1101 14 0.08243326051647415 1101 15 0.08199028167251754 1101 16 0.1343993192057482 1101 17 0.12754738991194614 1101 18 0.24588272631125818 1101 19 -0.003998268820652129 1101 20 0.01368407420008566 1101 21 0.1772300334855288 1101 22 -0.04936603386044425 1101 23 0.21021763210879385 1101 24 0.13200006480126092 1101 25 0.1089072236056694 1102 1 0.4457453039402921 1102 2 0.5062138081530598 1102 3 0.4048591673102777 1102 4 0.22436607524679886 1102 5 0.20352543594019323 1102 6 -0.23672870840903926 1102 7 0.22648961778057242 1102 8 0.36420313603275645 1102 9 0.5269840765003266 1102 10 0.6884389418139563 1102 11 0.24868539841945392 1102 12 0.4069627229772408 1102 13 0.3418482789337389 1102 14 0.675492860594133 1102 15 0.4574305567924668 1102 16 0.36192102095575507 1102 17 0.6146317285979137 1102 18 0.492581009584967 1102 19 0.43414273015319793 1102 20 0.31131698875204705 1102 21 0.4899141170199286 1102 22 0.5513231527052724 1102 23 0.5947629353786184 1102 24 0.4060467131635761 1102 25 0.4457599845120086 1103 1 -0.020485197614537043 1103 2 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0.9306073628585878 2202 18 1.0598180085142204 2202 19 0.9742882584674959 2202 20 0.9034626712101996 2202 21 0.8941061417269864 2202 22 1.0368921224462824 2202 23 1.177903588893514 2202 24 0.95925386874152 2202 25 1.1747723352883739 2203 1 -0.7188767784229846 2203 2 -0.5596520807614134 2203 3 -0.9144243114251935 2203 4 -1.128072075545024 2203 5 -0.6724627442731794 2203 6 -1.014696233843754 2203 7 -0.7006216980021662 2203 8 -0.6781383074139136 2203 9 -0.25452043053952167 2203 10 -0.28214164337634173 2203 11 -0.5443212278245682 2203 12 -0.5706747694541718 2203 13 -0.6593925428228573 2203 14 -0.3782142543034084 2203 15 -0.5521015979733974 2203 16 -0.6096274904845345 2203 17 -0.6785774372799125 2203 18 -0.705724479878711 2203 19 -0.5478781463530566 2203 20 -0.5333052641709202 2203 21 -0.6953414240380482 2203 22 -0.40885329129572057 2203 23 -0.4168671035942708 2203 24 -0.4544141391232017 2203 25 -0.47987598993815084 2204 1 -0.016309714215591242 2204 2 -0.0033427761002720047 2204 3 0.0052819458247768125 2204 4 -0.47019136324150423 2204 5 -0.16913457040555357 2204 6 -0.37029241399898893 2204 7 -0.14894529629423658 2204 8 -0.15955679953351876 2204 9 0.33465375061646796 2204 10 0.29541560086030216 2204 11 -0.01297694283521622 2204 12 -0.12813345303312929 2204 13 -0.28901509499609546 2204 14 0.213391740868353 2204 15 0.12142807815512668 2204 16 0.2828425913379391 2204 17 0.1683088711683709 2204 18 0.11336441085031623 2204 19 0.09534161503757588 2204 20 0.08067964810754802 2204 21 0.13006763524767706 2204 22 0.28117676666157365 2204 23 0.40887530388744864 2204 24 0.3740969692984777 2204 25 0.19100813299160535 2205 1 0.531354681611127 2205 2 0.38213323276484346 2205 3 0.34696664499031726 2205 4 0.16510148344897146 2205 5 0.023179492979359395 2205 6 -0.07282787609473473 2205 7 -0.23644018117541776 2205 8 -0.10860661506187341 2205 9 0.15601383206552555 2205 10 0.43532269393418943 2205 11 0.22620699520064136 2205 12 0.350210852571081 2205 13 0.1410974867230479 2205 14 0.7705982476268574 2205 15 0.4409611210042712 2205 16 0.3198089412218863 2205 17 0.4258633696903118 2205 18 0.34584255087853677 2205 19 0.37313856200729845 2205 20 0.3407221404225922 2205 21 0.39889798353407 2205 22 0.5283700805056242 2205 23 0.6939365379978426 2205 24 0.7274870245253879 2205 25 0.6670477706611422 2206 1 0.16950569202729326 2206 2 0.4383741326192414 2206 3 0.4722614497071902 2206 4 0.3433827359490138 2206 5 0.5390437468074225 2206 6 0.5092870377438885 2206 7 0.5902098452250044 2206 8 0.4746091163898285 2206 9 0.8912927965289583 2206 10 0.82736096654655 2206 11 0.5030434245835167 2206 12 0.6400554900852811 2206 13 0.23066691983397192 2206 14 0.6747791145207245 2206 15 0.41418629260701323 2206 16 0.5744722158342839 2206 17 0.43426670414365987 2206 18 0.498891399660117 2206 19 0.5988669736618846 2206 20 0.6520999224671087 2206 21 0.867939088191747 2206 22 0.7760060426810185 2206 23 0.8653760810305329 2206 24 0.7114067672412716 2206 25 0.5837376376990164 2207 1 -0.6734379318955668 2207 2 0.10747344311819518 2207 3 0.4698583840895658 2207 4 0.7026371020221447 2207 5 0.7517623791107795 2207 6 0.8253874459232478 2207 7 0.8559424976021077 2207 8 0.9486289272207102 2207 9 1.1553539511309978 2207 10 0.8655102905200209 2207 11 0.6996787383426408 2207 12 0.7372212398272922 2207 13 0.6769371069654824 2207 14 -4.609084983442319 2207 15 -1.2912028581038513 2207 16 -0.19738904262015922 2207 17 -0.04113736713326688 2207 18 0.46556529655703827 2207 19 0.6161051571651299 2207 20 0.8445647079593215 2207 21 0.7338860183017104 2207 22 0.753011938605141 2207 23 1.1107608936032491 2207 24 0.8890535244830033 2207 25 0.774674872727509 2208 1 0.7428242178735118 2208 2 0.8463689313338527 2208 3 0.8795992439515601 2208 4 0.6578539437857813 2208 5 0.8327042136165659 2208 6 0.7443378529333842 2208 7 0.8570955133817506 2208 8 0.7561703730353102 2208 9 0.9196696657408907 2208 10 1.0432889698066887 2208 11 0.6567407856777763 2208 12 0.8666448840670924 2208 13 0.595744892452565 2208 14 1.0030237740262962 2208 15 0.9796170318303652 2208 16 0.8699245130168123 2208 17 0.9059069444231369 2208 18 0.7757690108018167 2208 19 0.3516709082227507 2208 20 0.5325151542696034 2208 21 0.8893377735606407 2208 22 0.8954464689873234 2208 23 1.2676065365474116 2208 24 1.0877284754528986 2208 25 1.175022996018427 2209 1 -0.4509851456042533 2209 2 -0.29586537264916046 2209 3 0.16715540515098937 2209 4 0.33095900652020305 2209 5 0.41262385194448825 2209 6 0.18188507162138734 2209 7 0.38844172096131047 2209 8 0.5586026088477425 2209 9 0.6526488204603424 2209 10 0.47372291392522214 2209 11 0.37157523465040754 2209 12 0.4429429643867593 2209 13 0.5203522016349638 2209 14 0.5954788402931683 2209 15 0.6662103295930848 2209 16 0.41267003587368123 2209 17 0.5643647462710888 2209 18 0.6885492917048808 2209 19 0.43963085086338083 2209 20 0.5728735675816665 2209 21 0.5696732227579488 2209 22 0.6739458710165422 2209 23 0.925720003969623 2209 24 1.0815743727495857 2209 25 1.0291346736653963 2210 1 -0.7099571508552742 2210 2 -0.9066160147273585 2210 3 -0.30350988691263225 2210 4 -0.053325318680371936 2210 5 0.39960790496807874 2210 6 0.2509791031191426 2210 7 0.32135309394143263 2210 8 0.34317403821635395 2210 9 0.4815246918521865 2210 10 0.5289346637121213 2210 11 0.15718528915555652 2210 12 0.05097113909899065 2210 13 0.047597801254390504 2210 14 0.458823952616747 2210 15 0.2745970357493661 2210 16 0.5707677545255478 2210 17 0.45027641360970705 2210 18 0.5179162256193024 2210 19 0.38625012255627666 2210 20 0.5432884456600462 2210 21 0.6121089765738006 2210 22 0.4128031462054693 2210 23 0.4150784558950207 2210 24 0.3304977321194613 2210 25 0.37549209184837906 2211 1 -0.9828528948626385 2211 2 -0.20460456181952935 2211 3 0.37403464418 2211 4 0.6478991065160287 2211 5 0.7497748066399694 2211 6 0.578441622183341 2211 7 0.9854620404465706 2211 8 0.9488314293705642 2211 9 1.0383590275547547 2211 10 0.9672357197968395 2211 11 0.8346478082197564 2211 12 1.0162483973073861 2211 13 1.1727539118699184 2211 14 1.1788938786620378 2211 15 0.94815607002176 2211 16 0.964231251978859 2211 17 1.2152414505870581 2211 18 1.2192269792666792 2211 19 1.1046970737231447 2211 20 1.0301204792705931 2211 21 1.2219245214502052 2211 22 0.9434555015333714 2211 23 1.2429337694111737 2211 24 1.0726993953809512 2211 25 1.2857694939375293 2212 1 0.892280528396995 2212 2 0.03360140850478999 2212 3 0.472849962929633 2212 4 0.6483243015192528 2212 5 0.8207442512473264 2212 6 0.6346611987477644 2212 7 0.6707180371623025 2212 8 0.7407234005159538 2212 9 0.9229523065413261 2212 10 0.45095221736296764 2212 11 0.6983068311509584 2212 12 0.8379098834372343 2212 13 0.6371079087029443 2212 14 0.8779349906202825 2212 15 0.6553961145939269 2212 16 0.20558073294771617 2212 17 0.5123471921857927 2212 18 0.6217107045613872 2212 19 0.719778300630658 2212 20 0.6617315284759187 2212 21 0.7899586543456323 2212 22 0.8713952118142956 2212 23 1.047810316241275 2212 24 1.061658987193745 2212 25 0.5801627846536164 2213 1 0.4102338468116713 2213 2 0.24025516100629574 2213 3 -0.3285898186139562 2213 4 -1.0578850131098996 2213 5 -1.915975189336013 2213 6 -0.6515477544162067 2213 7 -0.28314339680293443 2213 8 -0.06540392723767496 2213 9 -0.7150871391682472 2213 10 -0.5966141361434083 2213 11 -0.5155220052268987 2213 12 -0.5627019785356424 2213 13 -0.403269395226836 2213 14 -7.215114362521344 2213 15 -3.1156962003276867 2213 16 -1.8641163263687162 2213 17 -1.1044923205591388 2213 18 -4.8440667577558685 2213 19 -2.7419195341928955 2213 20 -5.063418561726078 2213 21 -2.063481179774705 2213 22 -1.6328865075769983 2213 23 -4.4537053729906795 2213 24 -2.0326292057103643 2213 25 -1.6311160791627337 2214 1 0.8251743864341456 2214 2 0.6847213535640897 2214 3 -1.2267185488037562 2214 4 -1.653160908640249 2214 5 0.1796502294446256 2214 6 0.1256210761877412 2214 7 0.01749630841692351 2214 8 -0.08232395942488324 2214 9 -5.048921714733105 2214 10 -1.0026679824008227 2214 11 -0.3334944389754142 2214 12 -0.5673985353950499 2214 13 -0.6288356487829105 2214 14 -1.870584893309939 2214 15 -1.1914397010075746 2214 16 -0.6609050620887515 2214 17 -1.2083561318020841 2214 18 -0.3276049893857049 2214 19 -3.637900379283156 2214 20 -1.540969848437669 2214 21 -3.755389121046516 2214 22 -5.772209064202354 2214 23 -5.889108816027708 2214 24 -5.2001828847144225 2214 25 -2.8585505992181197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25161.0 3 19.0 4 513.0 5 3048.0 6 3796.0 7 3009.0 8 2175.0 9 1853.0 10 1573.0 11 1475.0 12 1390.0 13 1363.0 14 1329.0 15 1470.0 16 1400.0 17 1492.0 18 1652.0 19 1999.0 20 2689.0 21 3493.0 22 4321.0 23 5283.0 24 6288.0 25 7356.0 26 8532.0 27 9286.0 28 11085.0 29 13347.0 30 16166.0 31 20518.0 32 26117.0 33 33357.0 34 43404.0 35 57275.0 36 77458.0 37 114384.0 38 182609.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.25618366954631 19.965771405782423 9.489755809249466 30.2882891154218 2 12.663888825162312 21.622284291908507 41.32536305031086 24.38846383261832 3 17.732343073437903 25.911736873799214 29.495885068677772 26.860034984085107 4 11.53963613527429 18.43425472824848 38.49298236307041 31.533126773406817 5 12.182051215882602 39.5627066254921 35.953910866816344 12.301331291808957 6 30.646507318027727 39.145405153410955 18.143126003602898 12.064961524958417 7 27.26648686995758 31.983421939219518 22.579996610860757 18.170094579962146 8 23.813709522209102 39.98629794641549 20.17993640178902 16.020056129586383 9 26.433712477575238 15.391465591905973 20.044325260658674 38.130496669860115 10 14.759505937806425 30.15966993709538 34.54693762866203 20.533886496436168 11 33.74021554819448 22.412826823265675 25.719294272449595 18.127663356090263 12 23.85627894509766 25.04267856554152 32.31846021755677 18.782582271804053 13 29.432766936368132 23.033890652658435 25.626034671807478 21.907307739165958 14 20.08371201689158 23.910280684843478 27.534167584687413 28.47183971357753 15 21.84539704244551 33.70786516853933 22.850168632492966 21.5965691565222 16 20.685523115227113 28.594710581274178 29.96042467860281 20.759341624895903 17 20.510317583179962 30.109339433865983 28.65702277952652 20.723320203427537 18 20.439328894654235 28.13797650701278 31.55470827271359 19.8679863256194 19 23.506233641885945 27.051626422958837 27.214741724550144 22.227398210605074 20 23.40526780492839 28.543498950796366 28.260987971424967 19.790245272850278 21 22.013834343341685 27.268505512524293 27.048778530108187 23.668881614025832 22 22.84727348301884 29.966102180783594 26.73083124905939 20.455793087138176 23 21.136933052786073 29.541740960090813 28.208615935007824 21.112710052115286 24 23.25651727716273 27.516898767923585 28.86077364537527 20.365810309538414 25 21.842410030902244 28.603493845351192 28.62298691097976 20.93110921276681 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 147.0 1 147.0 2 159.5 3 172.0 4 172.0 5 172.0 6 284.5 7 397.0 8 397.0 9 397.0 10 688.0 11 979.0 12 979.0 13 979.0 14 1733.5 15 2488.0 16 2488.0 17 2488.0 18 4884.5 19 7281.0 20 7281.0 21 7281.0 22 12407.5 23 17534.0 24 17534.0 25 17534.0 26 26714.0 27 35894.0 28 35894.0 29 35894.0 30 49189.5 31 62485.0 32 62485.0 33 62485.0 34 70056.5 35 77628.0 36 77628.0 37 77628.0 38 81895.5 39 86163.0 40 86163.0 41 86163.0 42 89116.5 43 92070.0 44 92070.0 45 92070.0 46 93222.5 47 94375.0 48 94375.0 49 94375.0 50 87373.5 51 80372.0 52 80372.0 53 80372.0 54 68511.0 55 56650.0 56 56650.0 57 56650.0 58 47618.0 59 38586.0 60 38586.0 61 38586.0 62 31090.0 63 23594.0 64 23594.0 65 23594.0 66 17951.0 67 12308.0 68 12308.0 69 12308.0 70 8801.5 71 5295.0 72 5295.0 73 5295.0 74 3705.5 75 2116.0 76 2116.0 77 2116.0 78 1474.5 79 833.0 80 833.0 81 833.0 82 531.5 83 230.0 84 230.0 85 230.0 86 149.5 87 69.0 88 69.0 89 69.0 90 42.0 91 15.0 92 15.0 93 15.0 94 9.0 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08700201380279066 2 0.03740943262360521 3 0.032249510882418286 4 0.03267950436085053 5 0.023936303632728237 6 0.38541748783476787 7 0.1924937471781678 8 0.20625353848799957 9 0.21041014211284462 10 0.017343070296767165 11 0.003153285508503121 12 0.004013272465367608 13 0.0 14 0.07639550800146198 15 0.002006636232683804 16 0.004013272465367608 17 0.005876577538573998 18 0.004299934784322438 19 0.002723292030070877 20 0.0018633050732063897 21 1.433311594774146E-4 22 0.0 23 1.433311594774146E-4 24 1.433311594774146E-4 25 1.433311594774146E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 697685.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.01570411801599 #Duplication Level Percentage of deduplicated Percentage of total 1 92.08196090922321 73.6800293871912 2 5.796963918996281 9.276962994504414 3 0.9925591603389032 2.3826096027991217 4 0.3678721586950634 1.1774219921360005 5 0.1949672614269276 0.7800221351518455 6 0.11027762718916348 0.5294365192802988 7 0.07742145112534776 0.4336452347943285 8 0.05703776453018687 0.36511335121604005 9 0.04209206863428342 0.3031223858600439 >10 0.23129705994559 3.392219110723683 >50 0.024856306433444997 1.3729067760914955 >100 0.01891192788786139 3.150877289700299 >500 0.0023414767838145323 1.1758682611653775 >1k 0.0014409087900397121 1.9797649593858058 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2305 0.33037832259544064 No Hit GTCCTACAGTGGACATTTCTAAATT 2287 0.32779836172484716 No Hit CTTTAGGACGTGAAATATGGCGAGG 2136 0.30615535664375754 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2084 0.298702136350932 No Hit GTCCTACAGTGTGCATTTCTCATTT 1432 0.2052502203716577 No Hit CTGAAGGACCTGGAATATGGCGAGA 1192 0.17085074209707818 No Hit CTGTAGGACCTGGAATATGGCGAGA 1154 0.16540415803693642 No Hit GTATCAACGCAGAGTACTTTTTTTT 1147 0.16440083992059454 No Hit GTACATGGGAAGCAGTGGTATCAAC 905 0.12971469932706023 No Hit CCCATGTACTCTGCGTTGATACCAC 864 0.1238381217884862 No Hit GTCCTTCAGTGTGCATTTCTCATTT 717 0.10276844134530627 No Hit GAATATGGCAAGAAAACTGAAAATC 705 0.10104846743157728 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.433311594774146E-4 2 0.0 0.0 0.0 0.0 1.433311594774146E-4 3 0.0 0.0 0.0 0.0 1.433311594774146E-4 4 0.0 0.0 0.0 0.0 1.433311594774146E-4 5 0.0 0.0 0.0 0.0 1.433311594774146E-4 6 0.0 0.0 0.0 0.0 1.433311594774146E-4 7 0.0 0.0 0.0 1.433311594774146E-4 1.433311594774146E-4 8 0.0 0.0 0.0 1.433311594774146E-4 1.433311594774146E-4 9 0.0 1.433311594774146E-4 0.0 1.433311594774146E-4 1.433311594774146E-4 10 0.0 1.433311594774146E-4 0.0 1.433311594774146E-4 1.433311594774146E-4 11 0.0 1.433311594774146E-4 0.0 1.433311594774146E-4 1.433311594774146E-4 12 0.0 1.433311594774146E-4 0.0 1.433311594774146E-4 1.433311594774146E-4 13 0.0 1.433311594774146E-4 0.0 1.433311594774146E-4 1.433311594774146E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAGG 55 1.9053671E-4 13.862382 1 TCCAACG 110 1.9281288E-10 13.780914 18 TAGGACC 570 0.0 13.715326 4 CCAACGT 50 0.0015289002 13.264129 19 GAGGTGT 65 5.2654468E-5 13.200679 6 TAGGACT 130 1.2732926E-11 13.200679 4 CGAAATC 65 5.5351542E-5 13.130604 13 TGTAGGA 1250 0.0 12.969742 2 CTGTAGG 1230 0.0 12.784666 1 GCCTTAT 60 3.9783117E-4 12.707183 1 GGACCTG 840 0.0 12.598267 6 AGGACCT 880 0.0 12.350636 5 ACGAAAT 70 1.1073662E-4 12.192704 12 ACTGTTC 315 0.0 12.066413 8 AGGTGTG 120 9.977157E-9 11.874461 7 CACGTTT 120 1.03791535E-8 11.842974 18 GTAGGAC 1305 0.0 11.837652 3 TGTCCAC 250 0.0 11.760028 10 ATAGTAC 65 7.76406E-4 11.736476 3 TTATACT 130 2.4610927E-9 11.733936 4 >>END_MODULE