FastQCFastQC Report
Thu 26 May 2016
SRR1926693_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926693_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621967
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8110.13039277003442304No Hit
GTCCTAAAGTGTGTATTTCTCATTT7520.12090673620947735No Hit
CTGTAGGACGTGGAATATGGCAAGA6660.1070796360578616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGTGT304.1182284E-420.8538066
CCCTTAT450.001184186817.8228031
GACCTAC500.002396590816.0405221
CCTTATA603.7169267E-415.9830112
GTCCTAT851.5661462E-615.7260021
GTTCAGG801.5381293E-515.0379891
ATTTAGA1251.884473E-914.9711551
TAGGACC2600.014.8788864
ACGCACA400.004422275314.6323438
CCCACCC450.004470532814.6079373
TCCAGGA1252.201159E-814.2477132
GGACGGG450.00620872713.9025366
GATATAC1253.0413503E-813.9017871
CTGTAGG5800.013.8280371
AACGCAC450.00721954513.5894777
CTTGGAC652.4054035E-413.4842493
GCAGGAC652.4054035E-413.4842493
TGTAGGA6250.013.370932
GTATAAA1101.5335245E-613.3671021
TAGAGCT652.833965E-413.2256774
Produced by FastQC (version 0.11.5)