FastQCFastQC Report
Thu 26 May 2016
SRR1926693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926693_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621967
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA19020.3058040056787579No Hit
GTCCTACAGTGGACATTTCTAAATT18830.3027491812266567No Hit
CTTTAGGACGTGAAATATGGCGAGG18720.30098059864912446No Hit
GTCCTAAAGTGTGTATTTCTCATTT17040.27396951928317737No Hit
GTCCTACAGTGTGCATTTCTCATTT12600.20258309524460302No Hit
GTATCAACGCAGAGTACTTTTTTTT12150.1953479847001529No Hit
CTGTAGGACCTGGAATATGGCGAGA11270.18119932407989492No Hit
CTGAAGGACCTGGAATATGGCGAGA9700.15595682729148008No Hit
CCCATGTACTCTGCGTTGATACCAC8590.13811022128183648No Hit
GTACATGGGAAGCAGTGGTATCAAC7990.1284634072225697No Hit
GGTATCAACGCAGAGTACTTTTTTT7010.11270694425910056No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA508.9041176E-515.15883619
CTACCGT400.005145049714.3034064
TCCAACG602.6297625E-514.2114118
TAGGACC4600.012.4377454
ATTAACC550.00297655612.1381873
GGCGAGG5450.011.8211119
GATATAC2350.011.7644851
GTCCTGA657.7797286E-411.733281
CTGTAGG11850.011.5847581
TGTAGGA12050.011.4771652
CTACACT1001.838569E-611.4427264
ACGAAAT1001.9678955E-611.37827612
CAGGACT1201.2055352E-711.1248724
GGACCTG7250.011.0481496
CCCTAAT951.3048255E-511.0385471
GTAGGAC11900.010.8995983
GTTCTAG700.001446367210.8951891
GTCCAAG700.001446367210.8951891
ATAATAC803.6304214E-410.7292913
CTATAGG1252.1249434E-710.6772851
Produced by FastQC (version 0.11.5)