FastQCFastQC Report
Thu 26 May 2016
SRR1926692_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926692_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682914
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11010.16122088579235452No Hit
GTCCTAAAGTGTGTATTTCTCATTT9590.14042763803348593No Hit
CTGTAGGACGTGGAATATGGCAAGA8410.12314874200851059No Hit
CTTTAGGACGTGAAATATGGCGAGG7630.1117270988733574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG601.0186268E-619.995751
TAGGACC2400.016.5432264
TGTAGGA7400.016.2565982
CTGTAGG7100.015.5835421
CTTATAC505.1430915E-415.3168293
GTATTAT708.519053E-515.2348571
GTGTAGG708.519053E-515.2348571
GTCCTAT801.5793701E-514.9968131
GTCCTAC8300.014.9365841
TTACAGT805.04373E-714.7547694
TCCTACA9150.014.6414372
TTACACC450.005132754314.3076554
GTAGGAC7450.014.2447983
AGTGTCC551.5627072E-414.1887418
TCTTAGT700.001216047713.6703212
TCTTACA903.7825164E-513.6703212
TATTATA700.001216047713.6703212
ATAGTGC500.001226392913.6566638
GTGTAGC700.00147041413.33051
ACTGTCC603.266386E-413.0063468
Produced by FastQC (version 0.11.5)