##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926692_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 682914 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.957801421555278 26.0 2.0 30.0 2.0 31.0 2 15.66768290004305 16.0 2.0 30.0 2.0 31.0 3 19.117126021724552 19.0 10.0 30.0 2.0 31.0 4 21.15010821274714 27.0 2.0 35.0 2.0 37.0 5 21.50452033491772 28.0 2.0 35.0 2.0 35.0 6 22.346112394825703 30.0 2.0 35.0 2.0 37.0 7 22.224026744216694 30.0 2.0 35.0 2.0 37.0 8 22.300878295070827 30.0 2.0 35.0 2.0 37.0 9 24.84496144463285 31.0 12.0 37.0 2.0 39.0 10 25.44234559549226 31.0 11.0 37.0 2.0 39.0 11 22.68519608618362 29.0 2.0 37.0 2.0 39.0 12 23.713357758077883 30.0 2.0 37.0 2.0 39.0 13 20.538283004887877 26.0 2.0 35.0 2.0 39.0 14 23.691866032911904 30.0 2.0 37.0 2.0 40.0 15 24.71558058554957 31.0 2.0 38.0 2.0 40.0 16 25.084543588211694 31.0 2.0 38.0 2.0 40.0 17 24.4080220935579 30.0 2.0 38.0 2.0 40.0 18 24.47103002720694 30.0 2.0 37.0 2.0 40.0 19 24.821057995589488 30.0 2.0 38.0 2.0 40.0 20 25.043432994491255 31.0 2.0 38.0 2.0 40.0 21 22.993157264311467 30.0 2.0 37.0 2.0 40.0 22 23.879191230520973 30.0 2.0 37.0 2.0 40.0 23 23.829331365296362 30.0 2.0 38.0 2.0 40.0 24 22.97090556058303 30.0 2.0 38.0 2.0 40.0 25 22.76378577683281 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.9245677149251925 1101 2 9.581152487326909 1101 3 6.45761966039807 1101 4 7.7594749731063 1101 5 7.636207937560673 1101 6 7.088001106426525 1101 7 7.012081609356397 1101 8 5.3979783200384475 1101 9 5.272013298959408 1101 10 4.789241938957208 1101 11 7.621303044560303 1101 12 4.99344019761881 1101 13 9.40146313437269 1101 14 6.912607389400769 1101 15 6.094929997566524 1101 16 5.835830113758043 1101 17 2.2002901550529117 1101 18 4.482942857704167 1101 19 5.025447972544313 1101 20 2.949940854075578 1101 21 0.48957391908819403 1101 22 4.0830204303719775 1101 23 5.174776541505878 1101 24 6.328260162536125 1101 25 6.800240861379297 1102 1 5.292059497280302 1102 2 10.022110483468348 1102 3 6.55982691152829 1102 4 7.775115344029999 1102 5 7.795789160179034 1102 6 6.692450778261247 1102 7 6.650492261701551 1102 8 6.770980482823127 1102 9 5.93776613324513 1102 10 5.211252326954003 1102 11 7.81955206100136 1102 12 5.459811814619261 1102 13 9.630884068773508 1102 14 6.875949594403355 1102 15 5.214176475023766 1102 16 5.500094006640744 1102 17 6.174676182930565 1102 18 6.135620970337612 1102 19 4.64012276568884 1102 20 5.266301985065663 1102 21 4.610030431693389 1102 22 5.358139470011981 1102 23 5.733290281062207 1102 24 7.107294339723033 1102 25 6.860333988682346 1103 1 4.267146918002343 1103 2 8.266592946093523 1103 3 5.698220037880919 1103 4 7.3018329183310975 1103 5 6.478431652047437 1103 6 6.1922948094192805 1103 7 6.359801330898858 1103 8 6.10578788922135 1103 9 4.943029616360665 1103 10 4.408248046930744 1103 11 5.1645283381032705 1103 12 4.00590823835422 1103 13 7.638091392683194 1103 14 6.0992582630696965 1103 15 5.348073678528486 1103 16 4.9990655056613775 1103 17 2.559429353862736 1103 18 1.5725749398004751 1103 19 3.5337951953856077 1103 20 4.273191415440653 1103 21 5.996712553792797 1103 22 1.6840957376887573 1103 23 3.9515655113488997 1103 24 5.45277835310285 1103 25 1.2667478443735725 1104 1 5.480669643125207 1104 2 9.749037750480356 1104 3 6.983149608991614 1104 4 7.772142037049509 1104 5 7.522679826018944 1104 6 7.251357135238937 1104 7 7.244755350926596 1104 8 7.219796973158267 1104 9 6.258663680105396 1104 10 5.176388634024921 1104 11 8.127402366513497 1104 12 5.361976881599407 1104 13 9.154597322273226 1104 14 7.017712392972033 1104 15 6.503114739611487 1104 16 6.129724593163761 1104 17 6.45932995737261 1104 18 4.019246467144697 1104 19 4.764878937292359 1104 20 4.2281893998602875 1104 21 3.2438809180583767 1104 22 5.225615237883041 1104 23 5.490600826936394 1104 24 6.798845497396577 1104 25 5.8611650505899995 1105 1 3.0309363570078176 1105 2 8.820978831725506 1105 3 5.974137827489024 1105 4 5.170443668248005 1105 5 1.9486814785770363 1105 6 4.513863739850361 1105 7 5.388832841286675 1105 8 2.499950537400057 1105 9 3.120365927359561 1105 10 2.822590841932467 1105 11 6.060707033022776 1105 12 3.329362748293054 1105 13 4.537323312402844 1105 14 3.334333248929674 1105 15 3.5706208945472397 1105 16 3.968167366313626 1105 17 4.675605687252435 1105 18 2.1904998256598667 1105 19 2.259280475183157 1105 20 1.2421700975320924 1105 21 1.829998024845107 1105 22 3.271237645221749 1105 23 1.2244883855347553 1105 24 3.705380409091518 1105 25 4.567729191203583 1106 1 6.22488542661344 1106 2 10.60459479994171 1106 3 7.5815961336998825 1106 4 8.601761147092475 1106 5 8.180878847884124 1106 6 7.193741857064339 1106 7 7.039286815383775 1106 8 6.83606532719153 1106 9 6.00185006912967 1106 10 5.085023222799336 1106 11 7.621132163771804 1106 12 5.032441803757578 1106 13 4.4871981749199605 1106 14 5.447133747234986 1106 15 5.718443053421339 1106 16 5.52234684601105 1106 17 5.900968193144561 1106 18 5.9773284041429555 1106 19 5.804739611902871 1106 20 5.514422684454946 1106 21 5.537650490558558 1106 22 6.189342738261185 1106 23 6.666358377229667 1106 24 7.337650167346101 1106 25 6.387733231516933 1107 1 3.9638569294189345 1107 2 -13.127846671630197 1107 3 -5.116902995629287 1107 4 1.9182541402653115 1107 5 4.480240355941284 1107 6 4.817255497574028 1107 7 4.773744650039241 1107 8 5.254884255298997 1107 9 3.6130499364560187 1107 10 3.3473107376560236 1107 11 5.843936829461846 1107 12 3.129752398301374 1107 13 4.445774973615336 1107 14 3.876971498416612 1107 15 3.633082761539306 1107 16 3.5112202255958955 1107 17 4.03337511428666 1107 18 -0.4890775219467649 1107 19 2.009000370906712 1107 20 2.2709001990352284 1107 21 -0.8827411731137396 1107 22 2.0131728607079076 1107 23 -1.1245659158685406 1107 24 2.2362695321710007 1107 25 3.09273889778742 1108 1 5.3077130511062585 1108 2 10.380696859696085 1108 3 0.8602739069119174 1108 4 5.949870249579476 1108 5 7.376305153633421 1108 6 7.45364535965366 1108 7 7.245197621950826 1108 8 6.921991155352121 1108 9 5.724807799127412 1108 10 4.963387774015622 1108 11 7.054387441058992 1108 12 2.2735560685567044 1108 13 3.4551146907810733 1108 14 4.927223332535597 1108 15 5.081081873899688 1108 16 4.728653602190452 1108 17 -0.1110620078781075 1108 18 3.4258005284738537 1108 19 4.53972346096851 1108 20 4.804969421476372 1108 21 4.457141416373112 1108 22 1.7185498049412757 1108 23 4.234787586609453 1108 24 5.002003953087016 1108 25 5.447065804699577 1109 1 3.548942211641549 1109 2 -13.127846671630197 1109 3 -5.319871168655487 1109 4 2.112029417212735 1109 5 1.6508012843745519 1109 6 2.408910148532353 1109 7 3.5320179048308695 1109 8 3.55380459994468 1109 9 3.2432732527915213 1109 10 2.382851444531351 1109 11 0.9016509142165496 1109 12 1.3242556513346067 1109 13 2.8039497582117825 1109 14 3.3333843698778374 1109 15 3.262212355349753 1109 16 3.2127254128651046 1109 17 3.694739629848467 1109 18 3.631393728273032 1109 19 2.3045410777820408 1109 20 1.3003744385288094 1109 21 0.42829803912470155 1109 22 2.881894506566887 1109 23 3.305105264015406 1109 24 4.1968040846591315 1109 25 4.564256393831027 1110 1 3.038628642861621 1110 2 8.620884292836807 1110 3 5.999687458986088 1110 4 7.139868996720963 1110 5 7.766134833713934 1110 6 3.622293883276928 1110 7 6.279816443189944 1110 8 6.883636475237189 1110 9 5.047068479057856 1110 10 4.534796578995117 1110 11 2.800832971314147 1110 12 5.231472413403946 1110 13 9.317250690637891 1110 14 7.084101869967842 1110 15 6.348972624637291 1110 16 6.224938394828598 1110 17 6.620754678269822 1110 18 6.953936839480146 1110 19 5.633711254546991 1110 20 6.080972775282238 1110 21 4.162586119190941 1110 22 5.855303442017078 1110 23 6.294123516526476 1110 24 7.246000902170021 1110 25 7.474825569767955 1111 1 5.38704482744247 1111 2 9.467332048236804 1111 3 7.506291085284133 1111 4 8.159930940001509 1111 5 8.750798808295247 1111 6 7.988193854161896 1111 7 7.858361620011003 1111 8 7.96148964265705 1111 9 6.431422737236435 1111 10 5.899390593113797 1111 11 7.38987432731501 1111 12 7.038570573249679 1111 13 8.611835717073493 1111 14 6.613843505726162 1111 15 6.4153316846859845 1111 16 6.234955652867935 1111 17 7.496235181748858 1111 18 7.154239804571649 1111 19 6.165984632017022 1111 20 5.679151609077614 1111 21 8.569373718720037 1111 22 8.01595722211843 1111 23 7.961030698655755 1111 24 7.644520255225622 1111 25 8.452179140156705 1112 1 -0.8626539708293386 1112 2 8.02853192919285 1112 3 6.154994429803491 1112 4 0.4403369209339516 1112 5 4.31581835818595 1112 6 3.3163508279824896 1112 7 6.1391801498181735 1112 8 6.442511368178636 1112 9 5.173013205510195 1112 10 3.9073624330612517 1112 11 0.7914013738960861 1112 12 4.402469467988141 1112 13 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1 2.964208169088355 1204 2 -13.127846671630197 1204 3 -5.738328615463997 1204 4 0.7468698427446512 1204 5 3.4190282347291614 1204 6 3.654087453496892 1204 7 3.8987377629868973 1204 8 4.394106018384395 1204 9 2.7916243166213093 1204 10 2.53570605446043 1204 11 4.931987793649174 1204 12 2.828333665519004 1204 13 1.781573871686959 1204 14 3.07834890888493 1204 15 2.9511130645696113 1204 16 2.862831795843828 1204 17 3.356494948997401 1204 18 3.5105781254361546 1204 19 2.3207290210444533 1204 20 2.5590269207983134 1204 21 3.828085273167254 1204 22 0.6904937973470275 1204 23 2.26694923564661 1204 24 3.4098714605456557 1204 25 1.365603911561525 1205 1 2.9323482355887727 1205 2 2.981879014155341 1205 3 2.5553351418094863 1205 4 -18.61563015725535 1205 5 -5.971561956459533 1205 6 0.22510158483521536 1205 7 3.1950199741257173 1205 8 3.7802760100718444 1205 9 2.759768539397701 1205 10 2.6192575922825405 1205 11 5.373249223408109 1205 12 4.596342809326153 1205 13 7.216989499284633 1205 14 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2204 6 3.825786578532174 2204 7 4.831810723754607 2204 8 4.98832348945411 2204 9 3.5308643674257105 2204 10 3.14825544763006 2204 11 4.13777032257946 2204 12 4.5866218393829605 2204 13 2.7789503887628833 2204 14 4.064010777357975 2204 15 4.29781218960489 2204 16 3.915714945716818 2204 17 -0.7995994625182625 2204 18 2.697376036810695 2204 19 3.5118079089925196 2204 20 3.7631787695140915 2204 21 5.083622953099514 2204 22 4.6726790922948105 2204 23 3.2964101444809337 2204 24 5.058652860489207 2204 25 4.9123580747001085 2205 1 5.786510800667305 2205 2 9.781692802054012 2205 3 6.8500595424307384 2205 4 7.471540895376229 2205 5 7.5755304277116196 2205 6 7.08278262893549 2205 7 7.072761885793916 2205 8 6.708799000840536 2205 9 5.825571685042359 2205 10 5.16322798428499 2205 11 7.165650074350928 2205 12 6.5064148058698805 2205 13 9.394479573441345 2205 14 6.920234873797213 2205 15 6.109906924218734 2205 16 5.516516006370143 2205 17 6.378073896365823 2205 18 6.161948739471242 2205 19 4.961759722798838 2205 20 5.334838324307086 2205 21 -0.7883292005169551 2205 22 3.7624565166452726 2205 23 5.224372481260648 2205 24 6.5015710219491645 2205 25 6.781831981736964 2206 1 4.072745513486698 2206 2 8.50783797567271 2206 3 6.174918272502808 2206 4 2.3171499257322026 2206 5 5.71822602172778 2206 6 6.57459921006674 2206 7 6.518139561050521 2206 8 6.524621232768904 2206 9 5.284124316621309 2206 10 4.478046994986016 2206 11 3.9334227867335443 2206 12 5.128631037579861 2206 13 8.1959652963066 2206 14 0.9320141924257328 2206 15 2.8682015846249342 2206 16 3.9124376049724567 2206 17 4.9054679780430455 2206 18 -0.3340622618390867 2206 19 1.7766660888176204 2206 20 3.249635496178673 2206 21 3.7176703354079166 2206 22 -1.003928747604565 2206 23 -2.018002354948134 2206 24 1.5198057620670937 2206 25 3.116572099666641 2207 1 0.6431795121165855 2207 2 -13.127846671630197 2207 3 -6.143690035481107 2207 4 -5.0159723300526124 2207 5 0.4131200510964277 2207 6 1.2332633873434844 2207 7 2.2308585214699335 2207 8 2.4963479630664622 2207 9 0.3068788076392721 2207 10 0.06468024892863511 2207 11 -1.8903568028720699 2207 12 -2.951924389798929 2207 13 -0.04761204219955317 2207 14 -5.4765983394754905 2207 15 -1.801126741532471 2207 16 -0.8730150818293971 2207 17 0.2933833151222949 2207 18 0.24223623778289038 2207 19 -0.5926207080687469 2207 20 -0.7078136837069628 2207 21 -3.998510107499982 2207 22 -4.753620117680061 2207 23 -1.942739957395876 2207 24 -0.26756153061488064 2207 25 0.2921365605492632 2208 1 4.70996239274081 2208 2 5.975129997718154 2208 3 6.1385639830878915 2208 4 8.064981266718906 2208 5 3.354174386512483 2208 6 6.183985549501184 2208 7 6.680283083448149 2208 8 6.667419909508023 2208 9 5.084767920804733 2208 10 4.854032013162495 2208 11 6.749904795526351 2208 12 6.302011595258882 2208 13 8.715233029638153 2208 14 3.3997755648248607 2208 15 4.920693381008334 2208 16 5.490237266479387 2208 17 6.290692455031191 2208 18 1.4655191283860063 2208 19 3.689253424369742 2208 20 4.600037915703112 2208 21 7.051230888613755 2208 22 6.0093185492912475 2208 23 2.056206409151571 2208 24 3.4235876042839237 2208 25 4.811688652779008 2209 1 2.828555168243046 2209 2 4.513742508420522 2209 3 4.988152787748174 2209 4 0.6159843820514972 2209 5 4.8678757974228795 2209 6 4.9119600936772265 2209 7 5.471781578146096 2209 8 4.457909202379888 2209 9 4.149889320847851 2209 10 3.2630341750577827 2209 11 -0.390141679441836 2209 12 2.5886731977816133 2209 13 2.143736452695695 2209 14 3.5496365955572315 2209 15 3.706048257581724 2209 16 3.373030443350167 2209 17 0.933889285430201 2209 18 2.9285761530495655 2209 19 3.321765228426816 2209 20 3.5909598596543297 2209 21 5.674213760064973 2209 22 4.4349140565749785 2209 23 0.561494177883862 2209 24 -3.1417775955264773 2209 25 -0.5063459334652443 2210 1 -2.716679210403317 2210 2 -13.127846671630197 2210 3 -7.598501709241386 2210 4 -2.469267317640977 2210 5 -1.2757073773070644 2210 6 -1.7306247872276188 2210 7 -1.6359459939514593 2210 8 -19.751727314948937 2210 9 -12.519206095298061 2210 10 -7.133659268355814 2210 11 -9.538480362938614 2210 12 -4.70482000085552 2210 13 -4.832651953612485 2210 14 -4.0489663116818235 2210 15 -5.813375687986628 2210 16 -5.368320701965111 2210 17 -4.981320563710749 2210 18 -4.8181320702985815 2210 19 -9.852425375683563 2210 20 -6.902290636899615 2210 21 -5.025385366622405 2210 22 -9.403989842734772 2210 23 -7.515060697160868 2210 24 -9.586233564286363 2210 25 -7.496461528111277 2211 1 -0.4244999437864223 2211 2 3.821006943890085 2211 3 -0.13260110223648525 2211 4 0.6348107598522326 2211 5 0.06484834054926836 2211 6 -1.0429672202244475 2211 7 -19.62542890367977 2211 8 -13.18558974881137 2211 9 -9.56455293205594 2211 10 -7.780233098978723 2211 11 -9.765036015874635 2211 12 -5.712345346826673 2211 13 -7.088540766761012 2211 14 -5.557806293936938 2211 15 -6.03125906946919 2211 16 -11.59715938581402 2211 17 -8.14559058892147 2211 18 -6.45428078108942 2211 19 -9.152405899590468 2211 20 -7.3062464478083875 2211 21 -5.126006437591123 2211 22 -6.553522957503063 2211 23 -8.52543171673434 2211 24 -20.495961872787678 2211 25 -20.251062755105142 2212 1 -1.2378304289595583 2212 2 -13.127846671630197 2212 3 -7.575731011204903 2212 4 -18.61563015725535 2212 5 -18.87430509860417 2212 6 -19.702579213169773 2212 7 -9.652048247069299 2212 8 -9.110201139261271 2212 9 -5.079850838658196 2212 10 -5.501387261126673 2212 11 -5.330697062902143 2212 12 -3.156521406944762 2212 13 -5.719391818937115 2212 14 -3.7171553797870587 2212 15 -3.6653362107927094 2212 16 -3.742771575939667 2212 17 -4.043933178775447 2212 18 -3.0300089789708267 2212 19 -3.469422561327626 2212 20 -3.782656013244871 2212 21 -1.6821809202666422 2212 22 -2.798968639801746 2212 23 -2.9423202112625866 2212 24 -2.0682777556625673 2212 25 -2.728436313224041 2213 1 -17.478291830911644 2213 2 -13.127846671630197 2213 3 -8.298658804143242 2213 4 -5.492988647821388 2213 5 -18.87430509860417 2213 6 -10.256352798075433 2213 7 -19.62542890367977 2213 8 -10.205500899854597 2213 9 -8.047007758850391 2213 10 -7.003523505287998 2213 11 -6.2221788730174925 2213 12 -4.914150611210555 2213 13 -17.950092794979707 2213 14 -11.638317757781735 2213 15 -6.84753473417253 2213 16 -5.993488958873154 2213 17 -4.608929579304483 2213 18 -4.669453321104726 2213 19 -5.436173494678815 2213 20 -5.405249808761436 2213 21 -3.73097133060633 2213 22 -4.541754630325929 2213 23 -4.414874663724458 2213 24 -3.5195467784480563 2213 25 -4.254836340010801 2214 1 -17.478291830911644 2214 2 -13.127846671630197 2214 3 -12.09989408536896 2214 4 -18.61563015725535 2214 5 -18.87430509860417 2214 6 -19.702579213169773 2214 7 -15.825006517196137 2214 8 -14.60811591051388 2214 9 -13.772053085701819 2214 10 -13.798564448883463 2214 11 -14.128514670271981 2214 12 -12.3541707343402 2214 13 -17.950092794979707 2214 14 -16.048930218183003 2214 15 -22.230553602097057 2214 16 -17.445948563184682 2214 17 -21.9410050510026 2214 18 -17.03825151201545 2214 19 -14.776473548473321 2214 20 -13.478646433654344 2214 21 -11.085610608564531 2214 22 -12.10631102489866 2214 23 -12.01039676928123 2214 24 -10.96586683582886 2214 25 -10.687176799455722 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 147549.0 3 10.0 4 766.0 5 2728.0 6 3591.0 7 3560.0 8 3047.0 9 2840.0 10 2680.0 11 2469.0 12 2307.0 13 2460.0 14 3437.0 15 6852.0 16 9936.0 17 10579.0 18 11133.0 19 12911.0 20 15340.0 21 17652.0 22 18476.0 23 20027.0 24 21001.0 25 21185.0 26 17761.0 27 14782.0 28 15425.0 29 17914.0 30 17180.0 31 19728.0 32 24479.0 33 30477.0 34 36368.0 35 40670.0 36 39274.0 37 39565.0 38 26755.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.39792569784872 19.39425245445583 13.377459520047536 25.83036232764791 2 12.761459109773718 20.372875429055203 43.43994147364934 23.425723987521742 3 18.019415004253048 25.618399240039487 30.182353269879115 26.179832485828353 4 11.082728986565668 17.460215017238745 39.321489835085515 32.13556616111008 5 12.293915723902476 38.20373180663903 36.851125311082576 12.651227158375924 6 30.40292473916323 38.142728783013695 19.008399347843643 12.445947129979436 7 26.26644348868546 31.307336114441185 24.215511085116763 18.210709311756588 8 23.199604454952873 39.215058602787835 21.025374468327872 16.559962473931424 9 26.19693658116125 15.319586208311895 20.997274908784938 37.48620230174192 10 14.450390434648252 29.644805495798522 35.416641905463706 20.48816216408952 11 32.4955640660632 21.76363108553474 26.499951099940855 19.240853748461202 12 24.307645902781232 24.62435942086671 32.59988059107418 18.468114085277875 13 28.52713475862863 21.971974504511262 25.53352607913512 23.967364657724982 14 19.236537414338468 23.795679950961286 28.37371786669682 28.59406476800343 15 21.275831900322807 34.103237095363085 23.456421395601414 21.164509608712702 16 20.00917866082131 28.220456940305493 30.721642014112195 21.048722384761007 17 19.874029663496046 29.354526427533127 30.440820782542083 20.33062312642874 18 19.935331370974936 27.261023399637264 33.38402759194838 19.41961763743942 19 22.601547015175964 26.683783085056117 27.900653454931224 22.814016444836696 20 22.979354497665266 28.359666382778776 28.944377551320077 19.71660156823588 21 21.150048224865657 26.264792786172475 27.271544267364778 25.313614721597084 22 22.033141540632172 29.847627495512764 28.311400701883894 19.80783026197117 23 20.311512779353667 29.311080326318802 29.55471996833564 20.822686925991892 24 22.473450693685617 27.278637765915505 29.872656668667553 20.375254871731325 25 21.32040343624476 28.167971384173335 29.718386255020963 20.793238924560942 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 702.0 1 702.0 2 1491.0 3 2280.0 4 2280.0 5 2280.0 6 3280.0 7 4280.0 8 4280.0 9 4280.0 10 5081.5 11 5883.0 12 5883.0 13 5883.0 14 7378.0 15 8873.0 16 8873.0 17 8873.0 18 12966.5 19 17060.0 20 17060.0 21 17060.0 22 24114.5 23 31169.0 24 31169.0 25 31169.0 26 40708.5 27 50248.0 28 50248.0 29 50248.0 30 60731.5 31 71215.0 32 71215.0 33 71215.0 34 76971.0 35 82727.0 36 82727.0 37 82727.0 38 84372.0 39 86017.0 40 86017.0 41 86017.0 42 84794.0 43 83571.0 44 83571.0 45 83571.0 46 80572.0 47 77573.0 48 77573.0 49 77573.0 50 69899.0 51 62225.0 52 62225.0 53 62225.0 54 52295.5 55 42366.0 56 42366.0 57 42366.0 58 34806.5 59 27247.0 60 27247.0 61 27247.0 62 21435.5 63 15624.0 64 15624.0 65 15624.0 66 11849.0 67 8074.0 68 8074.0 69 8074.0 70 5817.0 71 3560.0 72 3560.0 73 3560.0 74 2499.5 75 1439.0 76 1439.0 77 1439.0 78 983.0 79 527.0 80 527.0 81 527.0 82 355.0 83 183.0 84 183.0 85 183.0 86 119.5 87 56.0 88 56.0 89 56.0 90 34.0 91 12.0 92 12.0 93 12.0 94 7.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.255256152312004 2 36.34981857159173 3 5.662499231235558 4 13.782701775040488 5 13.447227615775926 6 14.766866691852854 7 9.368090271981538 8 7.597735586032794 9 0.10762702185048191 10 1.4376627218068454 11 5.6731887177594835 12 5.820059333971774 13 16.077134163306066 14 1.5802868296740147 15 2.924819230532688 16 3.0030135566118132 17 2.8908471637717192 18 1.5015067783059066 19 1.4461557384970876 20 0.27734092433307855 21 4.352524622426836 22 1.6119159952790543 23 0.11114137358437519 24 9.008894238513195 25 0.1133378434180585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 682914.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.22149797355172 #Duplication Level Percentage of deduplicated Percentage of total 1 97.07156467278381 88.55013540087843 2 1.9762787900651408 3.6055822328620097 3 0.409081327372099 1.1195103442767533 4 0.1680463621211801 0.6131776352679987 5 0.08981624172312501 0.409658605616904 6 0.06181859154911453 0.33835107142352233 7 0.03658524915513548 0.23361528621669536 8 0.027649794199611253 0.2017804516439168 9 0.019539529423464177 0.16041826293360226 >10 0.12000235226420641 2.052608232027271 >50 0.010972396259017735 0.7071601100258951 >100 0.007813472595702229 1.365459807645595 >500 6.649763906969254E-4 0.47557551701512746 >1k 1.6624409767423135E-4 0.16696704216634417 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1101 0.16122088579235452 No Hit GTCCTAAAGTGTGTATTTCTCATTT 959 0.14042763803348593 No Hit CTGTAGGACGTGGAATATGGCAAGA 841 0.12314874200851059 No Hit CTTTAGGACGTGAAATATGGCGAGG 763 0.1117270988733574 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.464313222455536E-4 0.0 5 0.0 0.0 0.0 1.464313222455536E-4 0.0 6 0.0 0.0 0.0 1.464313222455536E-4 0.0 7 0.0 0.0 0.0 1.464313222455536E-4 0.0 8 0.0 0.0 0.0 1.464313222455536E-4 0.0 9 0.0 0.0 0.0 1.464313222455536E-4 0.0 10 0.0 0.0 0.0 1.464313222455536E-4 0.0 11 0.0 0.0 0.0 1.464313222455536E-4 0.0 12 0.0 0.0 0.0 1.464313222455536E-4 0.0 13 0.0 0.0 0.0 1.464313222455536E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTTAG 60 1.0186268E-6 19.99575 1 TAGGACC 240 0.0 16.543226 4 TGTAGGA 740 0.0 16.256598 2 CTGTAGG 710 0.0 15.583542 1 CTTATAC 50 5.1430915E-4 15.316829 3 GTATTAT 70 8.519053E-5 15.234857 1 GTGTAGG 70 8.519053E-5 15.234857 1 GTCCTAT 80 1.5793701E-5 14.996813 1 GTCCTAC 830 0.0 14.936584 1 TTACAGT 80 5.04373E-7 14.754769 4 TCCTACA 915 0.0 14.641437 2 TTACACC 45 0.0051327543 14.307655 4 GTAGGAC 745 0.0 14.244798 3 AGTGTCC 55 1.5627072E-4 14.188741 8 TCTTAGT 70 0.0012160477 13.670321 2 TCTTACA 90 3.7825164E-5 13.670321 2 TATTATA 70 0.0012160477 13.670321 2 ATAGTGC 50 0.0012263929 13.656663 8 GTGTAGC 70 0.001470414 13.3305 1 ACTGTCC 60 3.266386E-4 13.006346 8 >>END_MODULE