FastQCFastQC Report
Thu 26 May 2016
SRR1926692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926692_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682914
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA25740.376914223460055No Hit
CTTTAGGACGTGAAATATGGCGAGG23830.34894584091115427No Hit
GTCCTACAGTGGACATTTCTAAATT23660.34645650843297987No Hit
GTCCTAAAGTGTGTATTTCTCATTT23320.34147784347663107No Hit
GTCCTACAGTGTGCATTTCTCATTT14890.21803623882362932No Hit
GTATCAACGCAGAGTACTTTTTTTT13010.19050715024146525No Hit
CTGAAGGACCTGGAATATGGCGAGA12830.18787138644104528No Hit
CTGTAGGACCTGGAATATGGCGAGA11960.17513186140568213No Hit
CCCATGTACTCTGCGTTGATACCAC8740.12798097564261385No Hit
GAATATGGCAAGAAAACTGAAAATC7890.1155343132517418No Hit
GTCCTTCAGTGTGCATTTCTCATTT7800.11421643135153181No Hit
GTACATGGGAAGCAGTGGTATCAAC7750.11348427474030405No Hit
GGTATCAACGCAGAGTACTTTTTTT7660.11216639284009407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGA350.002113636416.3483145
TAGGACC4450.014.786964
TCCTAGA1009.46784E-913.3550622
GTAGCAC500.00145533713.3511253
CTCCTAG500.001457764413.3481731
AGGACCT8950.013.2126045
TGTCGAG957.627568E-812.96493318
GCCGGTT604.1552325E-412.64175411
GGACCTG9150.012.5069076
TATGTCG1001.4892794E-712.31668716
AGGCGTG550.00306513812.0910487
CTAGACA802.750635E-511.9206464
GGCGAGG5100.011.88941119
CTGTAGG14500.011.7700631
TGTAGGA14600.011.368792
GATATAC2300.011.1925681
GTAGGAC14350.011.1647033
TTACCCT600.0057046111.12593654
TCTAGGA1552.8921932E-1011.077932
TGCCGGT600.00595657711.06153510