FastQCFastQC Report
Thu 26 May 2016
SRR1926691_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926691_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855963
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14140.16519405628514316No Hit
GTCCTAAAGTGTGTATTTCTCATTT12680.14813724425004351No Hit
CTTTAGGACGTGAAATATGGCGAGG10870.12699147042570766No Hit
CTGTAGGACGTGGAATATGGCAAGA10720.12523905823032072No Hit
GTCCTACAGTGTGCATTTCTCATTT9190.10736445383737381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACA300.002445384622.2013681
GTCCTAG1003.1330273E-815.9849861
ATATAGG758.6371965E-615.9849861
TAGGACC3450.015.2504994
TGTAGGA8150.014.9226342
GGACCTA507.4621226E-414.5847266
GTCCTAC11550.014.4164721
TTAGCCT450.00511305214.3167944
TCCTACA12150.013.9462842
ATAGGAC1353.45608E-1113.7796513
CGTGCAA603.5358964E-513.7721839
AGTCGAC500.001230838613.6509718
GTCCTAA4400.013.6235681
TAGGACA2450.013.5863464
GACGTGC602.2633506E-413.58340557
CTGTAGG8400.013.4794021
GTGTAGG802.66817E-413.3208231
GTATTAT700.001479436613.3208211
ACAGTCG550.001533184813.2588416
TCCTAGA1451.1192242E-813.1938982
Produced by FastQC (version 0.11.5)