FastQCFastQC Report
Thu 26 May 2016
SRR1926691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926691_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855963
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA31410.36695511371402734No Hit
GTCCTACAGTGGACATTTCTAAATT31180.3642680816811007No Hit
CTTTAGGACGTGAAATATGGCGAGG29860.34884685436169555No Hit
GTCCTAAAGTGTGTATTTCTCATTT27220.3180043997228852No Hit
GTCCTACAGTGTGCATTTCTCATTT21010.24545453483386545No Hit
CTGTAGGACCTGGAATATGGCGAGA18340.21426159775597775No Hit
GTATCAACGCAGAGTACTTTTTTTT18210.21274284051997577No Hit
CTGAAGGACCTGGAATATGGCGAGA15720.18365279807655238No Hit
GGTATCAACGCAGAGTACTTTTTTT11770.13750594359802937No Hit
GAATATGGCAAGAAAACTGAAAATC9850.11507506749707638No Hit
TATCAACGCAGAGTACTTTTTTTTT9820.114724585057999No Hit
CCCATGTACTCTGCGTTGATACCAC9450.11040196830937785No Hit
GTCCTTCAGTGTGCATTTCTCATTT9160.10701397139829642No Hit
GTACATGGGAAGCAGTGGTATCAAC8980.10491107676383207No Hit
GGAATATGGCGAGAAAACTGAAAAT8570.1001211500964411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT1001.8189894E-1216.12471211
CGGTTTC951.8189894E-1115.97494613
CCGGTTT901.382432E-1015.80853912
TCCAACG1900.014.46799118
CCAACGA1250.014.40812519
TAACGTG400.00517647114.2911725
GGTCTAG602.5036363E-514.2869781
TATTGCG400.0053544614.21854416
TGCCGGT1152.5465852E-1114.02312710
TAGGACC6950.013.7085584
GTGCTAG801.9391246E-613.0963971
TAGTCAG751.4335636E-512.7032655
CCAACGT1001.4814941E-712.3227419
GGCGAGG7250.012.29005119
CTAGGAC1404.9112714E-1112.2510153
TGTAGGA17950.012.1583642
CTGTAGG18000.012.0116441
GTCTTAG1353.5470293E-1011.9940071
GGTTTCC1353.8016879E-1011.9442314
ATTATAC802.7761644E-511.9107093
Produced by FastQC (version 0.11.5)